DNA probe analysis of diarrhoeagenic Escherichia coli: detection of EAF-positive isolates of traditional enteropathogenic E. coli serotypes among Bangladeshi paediatric diarrhoea patients

Strockbine, Nancy; Faruque, Shah M.; B, Kay; Haider, Khaleda; Alam, Khorshed; Alam, Ahmed Nawsher; Tzipori, Saul; Wachsmuth, I K

doi:10.1016/0890-8508(92)90052-y

Cited by 22 publications

(15 citation statements)

References 12 publications

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“…In the present study, we did not isolate any EHEC strains from any of the groups of children. Similarly, no child with diarrhea was infected with EHEC in other studies (2,30,41), which concurs with the low prevalence of EHEC infection in the countries of the region evaluated in the present study (7,39). Some studies have suggested that there is an interesting phenomenon in developing countries, in which EHEC is much less frequently isolated than other diarrheagenic E. coli strains, such as ETEC or EPEC strains (25).…”

Section: Discussionsupporting

confidence: 72%

Detection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam

Nguyen

Van²,

Huy³

et al. 2005

J Clin Microbiol

238

175

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Diarrhea continues to be one of the most common causes of morbidity and mortality among infants and children in developing countries. Escherichia coli is an emerging agent among pathogens that cause diarrhea. The development of a highly applicable technique for the detection of different categories of diarrheagenic E. coli is important. We have used multiplex PCR by combining eight primer pairs specific for enteroaggregative E. coli (EAEC), enteroinvasive E. coli (EIEC), enterohemorrhagic E. coli, enteropathogenic E. coli (EPEC), and enterotoxigenic E. coli (ETEC). This facilitates the identification of five different categories of diarrheagenic E. coli from stool samples in a single reaction simultaneously. The prevalences of diarrheagenic E. coli were 22.5 and 12% in the diarrhea group and the control group, respectively. Among 587 fecal samples from Vietnamese children under 5 years of age with diarrhea, this technique identified 132 diarrheagenic E. coli strains. This included 68 samples (11.6%) with EAEC, 12 samples (2.0%) with EIEC, 39 samples (6.6%) with EPEC, and 13 samples (2.2%) with ETEC. Among the 249 age-matched controls, 30 samples were positive for diarrheagenic E. coli. The distribution was 18 samples (7.2%) with EAEC, 11 samples (4.4%) with EPEC, and 1 sample (0.4%) with ETEC.

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Section: Discussionsupporting

confidence: 72%

Detection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam

Nguyen

Van²,

Huy³

et al. 2005

J Clin Microbiol

238

175

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“…Comparatively, Vilchez et al [35] identified a few EHEC strains from children with diarrhea. Therefore, our finding is in concurrence with the low prevalence of EHEC infection in developing countries [47,35]. Evidently, the carrier rates of these pathogens have been judged to be as low as < 1% [9].…”

Section: Discussionsupporting

confidence: 65%

Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria

Ifeanyi

Ikeneche

Bassey

et al. 2015

J Infect Dev Ctries

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Introduction: Escherichia coli are frequently isolated from diarrheic children in the Federal Capital Territory Abuja, Nigeria, but their virulent properties are not routinely evaluated. Therefore, the etiology of childhood diarrheal disease attributable to diarrheagenic Escherichia coli (DEC) in Abuja, Nigeria remains unknown. Methodology: Stool specimens from 400 acute diarrheic children between 0 and 60 months of age were studied.E. coli strains isolated were evaluated by polymerase chain reaction (PCR) for nine virulence genes and HEp-2 cell adherence to detect and identify five distinct diarrheagenic E. coli categories. Results: Diarrheagenic E.coli was detected in 51 (12.8%) of the diarrheic children. The observed DEC pathotypes were enteropathogenic E. coli (EPEC) in 18 (4.5%) children, enterotoxigenic E. coli (ETEC) in 16 (4.0%), enteroaggrative E. coli (EAEC) in 8 (2.0%), enterohaemorrhagic E. coli (EHEC) in 6 (1.5%), and enteroinvasive E. coli (EIEC) in 3 (0.8%). Four (1.0 %) EPEC strains with only the eae+ gene that adhered diffusely to HEp-2 cell were identified as atypical EPEC. All the DEC categories except atypical EPEC were identified in children between 6 and 12 months of age. Conclusions: This study underscores the need for routine evaluation of diarrheic children for virulence properties of infectious DEC. Atypical EPEC are emerging among the DEC pathotypes isolated from childhood acute gastroenteritis in Abuja, Nigeria.

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“…In the present study, serogrouping was used in combination with two other methods to characterise EPEC isolates. With similar techniques, several studies of the association of EPEC serogroups with infantile diarrhoea have shown that, in each geographical area, a few clonal groups are responsible for a majority of the infections [28][29][30].…”

Section: Discussionmentioning

confidence: 99%

Clonal groups of enteropathogenic Escherichia coli isolated in case-control studies of diarrhoea in Bangladesh

Ansaruzzaman¹,

Albert²,

Nahar³

et al. 2000

Journal of Medical Microbiology

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Recent case-control studies in Bangladesh showed a high prevalence of enteropathogenic Escherichia coli (EPEC) strains (identified by DNA probes for virulence genes) associated with childhood diarrhoea. However, the clonal status of these strains is not known. A total of 94 EPEC isolates from 80 children with diarrhoea and 14 healthy matched controls isolated during 1991-1992 and 1993-1994 was characterised by serogrouping, enterobacterial repetitive intergenic consensus sequence PCR, and by a biochemical fingerprinting method (the phene plate or PhP system). Twelve O serogroups were found with O114 (n ¼ 19) and O127 (n ¼ 23) being the dominant serogroups. Most strains of O114 belonged to the same PhP=PCR types. Strains of O127 contained 16 that produced cytolethal distending toxin (CDT) and seven that did not; both were found among patients as well as controls. Results of PCR and PhP typing showed that CDT-positive strains belonged to the same clonal group and were related to one of the two PhP=PCR types of CDT-negative O127 strains. Thirty-one EPEC strains were O non-typable and 21 strains belonged to other less prevalent serogroups. These strains belonged to diverse PhP=PCR types and did not show any similarity to the strains of two major serogroups, O114 and O127. The results suggest that two clonal groups of EPEC strains are predominantly associated with childhood diarrhoea in Bangladesh.

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DNA probe analysis of diarrhoeagenic Escherichia coli: detection of EAF-positive isolates of traditional enteropathogenic E. coli serotypes among Bangladeshi paediatric diarrhoea patients

Cited by 22 publications

References 12 publications

Detection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam

Detection and Characterization of Diarrheagenic Escherichia coli from Young Children in Hanoi, Vietnam

Diarrheagenic Escherichia coli pathotypes isolated from children with diarrhea in the Federal Capital Territory Abuja, Nigeria

Clonal groups of enteropathogenic Escherichia coli isolated in case-control studies of diarrhoea in Bangladesh

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