DNA polymorphisms of casein genes in Spanish dairy sheep

Ordás, J.G.; Rando, A.; Senese, C.; Masina, P.

doi:10.1016/s0921-4488(97)00009-6

Cited by 5 publications

(3 citation statements)

References 12 publications

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“…Because of their great adaptation to the hot and semi‐arid environment of Sardinia and their remarkable milk yields, Sarda sheep have been exported to many European and North African countries to be crossed with local populations (Ordás et al . ; Carta et al . ; Vacca et al .…”

Section: Introductionmentioning

confidence: 99%

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Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

et al. 2016

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Regulatory variation at the ovine casein genes could have important effects on the composition and coagulation properties of milk. Herewith, we have partially resequenced the promoters and the 3'-UTR of the four casein genes in 25 Sarda sheep. Alignment of these sequences allowed us to identify a total of 29 SNPs. This level of polymorphism (one SNP every 250 bp) is remarkably high if compared with SNP densities estimated in human genic regions (approximately one SNP per bp). The 29 SNPs identified in our resequencing experiment, plus three previously reported SNPs mapping to the lactalbumin, alpha (LALBA) and β-lactoglobulin (BLG, also known as progestagen-associated endometrial protein, PAEP) genes, were genotyped with a multiplex TaqMan Open Array Real-Time PCR assay in 760 Sarda sheep with records for milk composition and coagulation properties. Association analysis revealed the existence of significant associations of CSN1S2 and CSN3 genotypes with milk protein and casein contents. Moreover, genotypes at CSN1S1 were significantly associated with rennet coagulation time, curd firming time and curd firmness, whereas CSN2 was associated with curd firming time. These results suggest that SNPs mapping to the promoters and 3'-UTRs of ovine casein genes may exert regulatory effects on gene expression and that they could be used for improving sheep milk quality and technological traits at the population level through marker assisted selection.

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Section: Introductionmentioning

confidence: 99%

“…Polymorphisms of the sheep milk protein genes have been investigated both at the protein and genomic levels (Ordás et al . ), and their current nomenclature is based mainly on criteria previously established for other ruminant species (Calvo et al . ).…”

Section: Introductionmentioning

confidence: 99%

Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

et al. 2016

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“…Casein gene variability has been established as the key determinant to qualitative and quantitative differences in the production of milk for many species. Already well-studied in farm animals, such as cattle (Caroli et al, 2009), sheep (Ordás et al, 1997;Giambra et al, 2010), goats (Caroli et al, 2006;Cosenza et al, 2008), and buffalo (Cosenza et al, 2008;Pauciullo et al, 2021), a rising interest in camel milk has prompted study of the different variants of these genes in camels.…”

Section: Introductionmentioning

confidence: 99%

Tunisian camel casein gene characterization reveals similarities and differences with Sudanese and Nigerian populations

Letaief

Bedhiaf-Romdhani

Salem³

et al. 2022

Journal of Dairy Science

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Milk is a primary protein source that has always played a role in mammalian health. Despite the intensification of research projects on dromedary and the knowledge of the genetic diversity at the casein loci, the genetic structure of the Tunisian camel population still needs exploration. This study sought to determine the genetic diversity of 3 casein gene variants in 5 Tunisian camel ecotypes: c.150G>T at CSN1S1 (α S1 -casein), g.2126A>G at CSN2 (β-casein), and g.1029T>C at CSN3 (κ-casein). The obtained results were compared with data published on Sudanese and Nigerian camels to establish the level of differentiation within and between populations. A total of 159 blood samples were collected from 5 Tunisian camel ecotypes and the extracted DNA was genotyped by PCR-RFLP. A streamlined genotyping protocol was also developed for CSN3. Results indicated that allele T was quite rare (0.06) at CSN1S1 for all ecotypes. Minor allele frequency was found for G (0.462) in CSN2 except for Ardhaoui Medenine ecotype who deviated from the average CSN2 allele frequency of the total population. Allele C showed minor allele frequency of 0.384 in CSN3. Among the Tunisian population, GAT (0.343) was the most represented haplotype in all ecotypes except for Ardhaoui Medenine, where GGC (0.322) was the most frequent one. Significant differences in heterozygosity and local inbreeding were observed across the Tunisian, Sudanese, and Nigerian populations, although the global fixation index indicated that only 2.2% of the genetic variance is related to ecotype differences. Instead, phylogenetic analysis revealed a closer link between the Tunisian and Sudanese populations through a clade subdivision with 3 main branches among the ecotypes.This study represents the first attempt to understand casein gene variability in Tunisian camels; with further study, milk traits and genetic differentiation among populations can be associated with the history of camel domestication.

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A short note on DNA lactotypes in the Churra and Manchega sheep breeds

Ordás

2001

Small Ruminant Research

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DNA polymorphisms of casein genes in Spanish dairy sheep

Cited by 5 publications

References 12 publications

Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

Variations at regulatory regions of the milk protein genes are associated with milk traits and coagulation properties in the Sarda sheep

Tunisian camel casein gene characterization reveals similarities and differences with Sudanese and Nigerian populations

A short note on DNA lactotypes in the Churra and Manchega sheep breeds

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