DNA polymerase from mesophilic and thermophilic bacteria

Stenesh, J.; Roe, Bruce A.

doi:10.1016/0005-2787(72)90240-7

Cited by 36 publications

(10 citation statements)

References 27 publications

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“…Several strains analyzed in this study were previously mis-identified as G. stearothermophilus , for example, strain BGSC 9A21. This strain was isolated prior to the 1980s when it was believed that G. stearothermophilus was the only obligate thermophile of the Bacillus genus [60]. Although, the 16S rRNA gene sequence of this strain is approximately 98% to the type strain of G. stearothermophilus ATCC 12980, it is also 98–99% similar to other type strains of the Geobacillus genus and based on other genomic evidence is actually more closely related to G. thermoleovorans as demonstrated in this study.…”

Section: Discussionsupporting

confidence: 51%

Insights into the Geobacillus stearothermophilus species based on phylogenomic principles

et al. 2017

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BackgroundThe genus Geobacillus comprises bacteria that are Gram positive, thermophilic spore-formers, which are found in a variety of environments from hot-springs, cool soils, to food manufacturing plants, including dairy manufacturing plants. Despite considerable interest in the use of Geobacillus spp. for biotechnological applications, the taxonomy of this genus is unclear, in part because of differences in DNA-DNA hybridization (DDH) similarity values between studies. In addition, it is also difficult to use phenotypic characteristics to define a bacterial species. For example, G. stearothermophilus was traditionally defined as a species that does not utilise lactose, but the ability of dairy strains of G. stearothermophilus to use lactose has now been well established.ResultsThis study compared the genome sequences of 63 Geobacillus isolates and showed that based on two different genomic approaches (core genome comparisons and average nucleotide identity) the Geobacillus genus could be divided into sixteen taxa for those Geobacillus strains that have genome sequences available thus far. In addition, using Geobacillus stearothermophilus as an example, we show that inclusion of the accessory genome, as well as phenotypic characteristics, is not suitable for defining this species. For example, this is the first study to provide evidence of dairy adaptation in G. stearothermophilus - a phenotypic feature not typically considered standard in this species - by identifying the presence of a putative lac operon in four dairy strains.ConclusionsThe traditional polyphasic approach of combining both genotypic and phenotypic characteristics to define a bacterial species could not be used for G. stearothermophilus where many phenotypic characteristics vary within this taxon. Further evidence of this discordant use of phenotypic traits was provided by analysis of the accessory genome, where the dairy strains contained a putative lac operon. Based on the findings from this study, we recommend that novel bacterial species should be defined using a core genome approach.Electronic supplementary materialThe online version of this article (doi:10.1186/s12866-017-1047-x) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 51%

Insights into the Geobacillus stearothermophilus species based on phylogenomic principles

et al. 2017

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“…MKK DNA polymerase has 94% homology with DNA polymerase of B. stearothermophilus. Bst DNA polymerase has been isolated by several groups as an exonuclease deficient polymerase (Stenesh and Roe, 1972;Sellmann et al, 1992;Uemori et al, 1993). In addition, bioinformatics studies on Geobacillus sp.…”

Section: Discussionmentioning

confidence: 99%

Restoring 3′–5′ exonuclease activity of thermophilic Geobacillus DNA polymerase I using site-directed mutagenesis in active site

Rastgoo

Sadeghizadeh

Bambaei

et al. 2009

Journal of Biotechnology

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“…A gene encoding a homologue of PTE was discovered in the genome Geobacillus stearothermophilus strain 10 [12; 13; 14; 15]. This strain was obtained from the Bacillus Genetic Stock Center (BGSC Accession No.…”

Section: Methodsmentioning

confidence: 99%

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus

Hawwa¹,

Aikens²,

Turner³

et al. 2009

Archives of Biochemistry and Biophysics

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A new enzyme homologous to phosphotriesterase was identified from the bacterium Geobacillus stearothermophilus (GsP). This enzyme belongs to the amidohydrolase family and possesses the ability to hydrolyze both lactone and organophosphate (OP) compounds, making it a phosphotriesterase-like lactonase (PLL). GsP possesses higher OP-degrading activity than recently characterized PLLs, and it is extremely thermostable. GsP is active up to 100°C with an energy of activation of 8.0 kcal/mol towards ethyl paraoxon, and it can withstand an incubation temperature of 60°C for two days. In an attempt to understand the thermostability of PLLs, the X-ray structure of GsP was determined and compared to those of existing PLLs. Based upon a comparative analysis, a new thermal advantage score and plot was developed and reveals that a number of different factors contribute to the thermostability of PLLs.

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DNA polymerase from mesophilic and thermophilic bacteria

Cited by 36 publications

References 27 publications

Insights into the Geobacillus stearothermophilus species based on phylogenomic principles

Insights into the Geobacillus stearothermophilus species based on phylogenomic principles

Restoring 3′–5′ exonuclease activity of thermophilic Geobacillus DNA polymerase I using site-directed mutagenesis in active site

Structural basis for thermostability revealed through the identification and characterization of a highly thermostable phosphotriesterase-like lactonase from Geobacillus stearothermophilus

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