DNA Ploidy in Testicular Germ Cell Neoplasms Histogenetic and Clinical Implications

El‐Naggar, Adel K.; Ro, Jae Y.; McLemore, Donia; Ayala, Alberto G.; Batsakis, John G.

doi:10.1097/00000478-199206000-00009

Cited by 59 publications

(25 citation statements)

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“…In conclusion, our study of CGH on a limited number of TGCTs largely confirms our karyotypic findings on a series of more than 100 tumors [5]: overrepresentation of (parts of) chromosomes 7,8,12, and X, and underrepresentation of chromosome 13. In addition, through CGH the most likely regions involved could be identified, i.e., 7 q ll and 13q31-qter.…”

Section: Resultssupporting

confidence: 86%

“…In spite of its technical limitations in the study of solid tumors in general, karyotyping has been highly infor mative in the detection of chromosomal aberrations in TGCT [3][4][5]. In accordance with results of total DNA con tent analysis [6,7], TGCT are found to be aneuploid, around the triploid range. Besides consistent over-and underrepresentations of some (parts of) chromosomes, the only recurrent structural abnormality is the isochromo some 12p [i(12p]] [5], first reported in TGCT by Atkin and Baker in 1983 [8].…”

Section: Introductionmentioning

confidence: 98%

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Comparative genomic hybridization of germ cell tumors of the adult testis: Confirmation of karyotypic findings and identification of a 12p-amplicon

Mostert

Pol

Weghuis

et al. 1996

Cancer Genetics and Cytogenetics

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Comparative genomic hybridization (CGH) was carried out on 15 primary testicular germ cell tumors (TGCT) o f adolescents and adults and two metastatic residual tumors after chemotherapeu tic treatment. The results were compared with karyotypic data obtained form the same tum or specimens after direct harvesting o f metaphases or short-term in vitro culture. .Both techniques revealed that the m ost consistent abnormality in prim ary TGCT is gain o f 12p-sequences. Although In m o st cases over representation o f the complete short arm was observed, CGH revealed a specific amplification of 1 2 p ll.l-p l2 ,l region in two independent primary tumors. In additiont loss of (parts of) chromosome 13 (always involving q31-qter), and gain o f (parts of) chromosome 7 (mostly involving q ll), (parts of) chro mosome 8, and the X chromosome were detected in more than 25% o f the tumors by this latter tech nique. Loss of 6ql5-q21 in both residual tumors analyzed m a y suggest a role for this anomaly in acquired resistance to chemotherapeutic treatm ent Overall, the CGH analyses confirmed gains and losses o f certain chromosomal regions in TGCT as

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Section: Resultssupporting

confidence: 86%

Section: Introductionmentioning

confidence: 98%

Comparative genomic hybridization of germ cell tumors of the adult testis: Confirmation of karyotypic findings and identification of a 12p-amplicon

Mostert

Pol

Weghuis

et al. 1996

Cancer Genetics and Cytogenetics

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“…Despite a hypertriploid DNA content for CIS and SE and a hypotriploid DNA content for NS [4][5][6], they show a striking similarity in chromosome constitution [7], supporting a common patho genetic origin. The only recurrent chromosome abnormal ity detected both in SE and NS by conventional karyotyping is an isochroniosome of the short arm of chromosome 12 [i(12p)L present in ^70% of all TGCT [8].…”

Section: Introductionmentioning

confidence: 87%

Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults

Mostert

Pol

Echten

et al. 1996

Cancer Genetics and Cytogenetics

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Overrepresentation o f the short arm of chromosome '12 is frequentty detected in human testicular germ cell tumors of adolescents and adults (TGCT). This overrepresentation m ostly results from the formation of an isochromosome: i{ 12p). Whether the overrepresentation consistently involves I N T R O D U C T I O NHuman testicular germ cell tumors of adolescents and adults (TGCT) can clinically and histologically be grouped Rotterdam; Department of Medical Genetics University Hospital Groningen (J. v E.}, Groningen; and Department of Human Genetics, University Hos pital Nijmegen, (D. O. W., A. G. v K.j into two entities; the seminomas (SE) and nonseminomatous TGCT (NS) [l, 2], All TGCT originate from a common precursor, carcinoma in situ (CIS) [3]. Despite a hypertriploid DNA content for CIS and SE and a hypotriploid DNA content for NS [4-6], they show a striking similarity in From the Laboratory for Experimental Patho-Oncology Dr. Daniel den Hoed Cancer Center; University Hospital Rotterdam

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“…The fact that CIS and seminoma cells share several morphological and immunohistochemical features, whereas the EC cell phenotype is closer to that of ES cells, has led to the suggestion that CIS gives rise to seminomas as an early stage in the development of non-seminomas. 19 The origin of non-seminomas of stem cell tumors. 8 The functional equivalence between ES and EC cells is further supported by the induction of experimental teratocarcinomas through the transplantation of ES cells into immunodeficient or syngenic mice and the later isolation from these experimental tumors of a stem cell population that retains the properties of the starting ES cells.…”

Section: Factors Involved In the Progression From Cis To Type II Tgctsmentioning

confidence: 99%

The role of microenvironment in testicular germ cell tumors

Díez-Torre¹,

Silván²,

Díaz-Núñez³

et al. 2010

Cancer Biology & Therapy

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DNA Ploidy in Testicular Germ Cell Neoplasms Histogenetic and Clinical Implications

Cited by 59 publications

References 0 publications

Comparative genomic hybridization of germ cell tumors of the adult testis: Confirmation of karyotypic findings and identification of a 12p-amplicon

Comparative genomic hybridization of germ cell tumors of the adult testis: Confirmation of karyotypic findings and identification of a 12p-amplicon

Fluorescence in situ hybridization-based approaches for detection of 12p overrepresentation, in particular i(12p), in cell lines of human testicular germ cell tumors of adults

The role of microenvironment in testicular germ cell tumors

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