DNA ploidy analysis by image cytometry helps to identify oral epithelial dysplasias with a high risk of malignant progression

Torres-Rendon, A.; Stewart, R.; Craig, Gavin A.; Wells, Michael; Speight, Paul M.

doi:10.1016/j.oraloncology.2008.07.006

Cited by 107 publications

(106 citation statements)

References 22 publications

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“…A clear correlation between histologic grade of the leukoplakias and DNA ploidy status was found in the present study. This is in contrast to the results from Torres-Rendon et al [10] and Bremmer et al [12] who found no correlation between histologic grade and DNA aneuploidy. The aneuploid dysplastic lesions in the study of Torres-Rendon et al [10] had a significantly increased risk to progress to OSCC compared to diploid dysplastic lesions.…”

Section: Discussioncontrasting

confidence: 92%

“…This is in contrast to the results from Torres-Rendon et al [10] and Bremmer et al [12] who found no correlation between histologic grade and DNA aneuploidy. The aneuploid dysplastic lesions in the study of Torres-Rendon et al [10] had a significantly increased risk to progress to OSCC compared to diploid dysplastic lesions. Donadini and co-workers, on the other hand, found aneuploid clones in 23 % of PMDs with no histologic evidence of dysplasia compared to 46 % of PMDs with dysplasia using highresolution flow cytometry [8].…”

Section: Discussioncontrasting

confidence: 92%

“…Several recent studies have reported the potential use of DNA ploidy analysis to predict the behaviour of oral PMD [6][7][8][9][10][11][12]. Aneuploidy in oral dysplastic lesions, as detected by DNA image cytometry, was found to be indicative of a high risk of OSCC development [10].…”

Section: Introductionmentioning

confidence: 99%

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Correlation Between Dysplasia and Ploidy Status in Oral Leukoplakia

Zyl

Heerden

Langenegger

et al. 2012

Head and Neck Pathol

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Oral leukoplakia and other potentially malignant disorders (PMD) may progress to oral squamous cell carcinoma (OSCC). The gold standard for assessing the potential for malignant transformation remains histologic examination with the aim of grading the dysplastic changes. However, not all lesions with dysplasia will progress to OSCC. DNA ploidy has been suggested as a method to predict the clinical behaviour of PMD. This study reports on the use of high-resolution flow cytometry to determine the ploidy status of formalin-fixed, paraffin-embedded material from PMD compared to their dysplasia grade on histology. Aneuploidy was found in 13 % of mild, 31 % of moderate, and 54 % of severe dysplasia cases. This difference was statistically significant (p = 0.011). The differences in ploidy status were more significant when grouping the dysplasia into low-risk and high-risk categories (p = 0.008). These findings indicate that the ploidy status of PMD as determined by high-resolution flow cytometry may be of value in predicting biological behaviour in PMD such as leukoplakia.

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Section: Discussioncontrasting

confidence: 92%

Section: Discussioncontrasting

confidence: 92%

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Correlation Between Dysplasia and Ploidy Status in Oral Leukoplakia

Zyl

Heerden

Langenegger

et al. 2012

Head and Neck Pathol

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“…Image-based DNA ploidy analysis is predictive in crosssectional (9,15) and case-control studies (16,17) of oral dysplastic lesions. Data from a flawed retrospective study were retracted (18) and the predictive value of ploidy analysis remains unknown in a routine diagnostic setting.…”

Section: Introductionmentioning

confidence: 99%

Predictive Value of Dysplasia Grading and DNA Ploidy in Malignant Transformation of Oral Potentially Malignant Disorders

Sperandio

Brown

Lock

et al. 2013

Cancer Prevention Research

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Dysplasia grading is widely used to assess risk of transformation in oral potentially malignant disorders despite limited data on predictive value. DNA ploidy analysis has been proposed as an alternative. This study examines the prognostic value for both tests used in a routine diagnostic setting to inform clinical management. A retrospective study of conventional dysplasia grading was conducted on 1,401 patients. DNA ploidy analysis was conducted on a subset of 273 patients and results correlated with clinical information, pathologic diagnosis, and outcome over 5 to 15 years.Malignant transformation occurred in 32 of 273 patients (12%) and, of these, 20 (63%) of preexisting index lesions were aneuploid. Of 241 patients not developing carcinoma, only 39 (16%) of index lesions were aneuploid. Epithelial dysplasia correlated with DNA ploidy status (P < 0.001). The overall positive predictive value for malignant transformation by DNA aneuploidy was 38.5% (sensitivity 65.2% and specificity 75%) and by severe dysplasia grade 39.5% (sensitivity 30% and specificity 98%). DNA diploid and tetraploid status had negative predictive value of 90% to 96%. Combining DNA ploidy analysis with dysplasia grading gives a higher predictive value than either technique alone.Each of three traditional dysplasia grades predicts a significantly different risk of carcinoma development and time to transformation. DNA ploidy analysis had equivalent predictive value and also detected additional risk lesions in the absence of dysplasia. Cancer Prev Res; 6(8); 822-31. Ó2013 AACR.

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“…Aneuploidy may result from a gene dose imbalance, loss of TSGs, gain of tumour promoting genes or oncogenes, or formation of fusion genes that leads to an increased survival and proliferation advantage. Approximately50-60% of oral cancers are aneuploid, with one study reporting a figure of 90% [6,7,8]. Aneuploidy in OSCC has also been shown to be associated with higher incidences of local recurrence and lymph node metastases.…”

Section: Aneuploidymentioning

confidence: 99%

Early Detection of Oral Squamous Cell Carcinoma ( O SCC ) – Role of Genetics: A Literature Review

Seethalakshmi¹

2013

JCDR

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According to GLOBOCAN 2008, there were 12.7 million new cancer cases and 7.6 million deaths. Among these, 56% of the cases and 64% of the deaths occurred in the economically developing world. Oral cancer accounted for 3-4% of all cancers. There were 263,900 oral cancer cases, with almost two-thirds of them occurring in men. Oral cancer is among the leading cancer types in south central Asian men. In India, oral cancer is the leading cancer type among men and third most common cancer among women [1].

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DNA ploidy analysis by image cytometry helps to identify oral epithelial dysplasias with a high risk of malignant progression

Cited by 107 publications

References 22 publications

Correlation Between Dysplasia and Ploidy Status in Oral Leukoplakia

Correlation Between Dysplasia and Ploidy Status in Oral Leukoplakia

Predictive Value of Dysplasia Grading and DNA Ploidy in Malignant Transformation of Oral Potentially Malignant Disorders

Early Detection of Oral Squamous Cell Carcinoma ( O SCC ) – Role of Genetics: A Literature Review

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