DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA

Wen, Yanli; Pei, Hao; Ye, Shen; Xi, Junjie; Lin, Meihua; Lü, Na; Shen, Xizhong; Li, Jiong; Fan, Chunhai

doi:10.1038/srep00867

Cited by 196 publications

(138 citation statements)

References 45 publications

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“…Of significance, this non-optimized, proof-ofconcept sensor exhibits a 250-fold improvement in sensitivity (B1 pM) compared with that of 1D probes with the similar HRPbased signal-transduction approach. To further improve the sensitivity, Wen et al 61 increased the interprobe distance using interfacial engineering and multienzyme amplification, which results in an extremely low detection limit of 10 aM (B600 molecules in 100 ml).…”

Section: D-structured Dna Probesmentioning

confidence: 99%

Scaffolded biosensors with designed DNA nanostructures

et al. 2013

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In addition to its fundamental function as a genetic code carrier, the utilization of DNA in various material applications has been actively explored over the past several decades. DNA is intrinsically an excellent type of self-assembly nanomaterial owing to its predictable base-pairing, high chemical stability and the convenience it possesses for synthesis and modification. Because of these unparalleled properties, DNA is widely used as excellent recognition elements in biosensors and as unique building blocks in nanodevices. A critical challenge in surface-based DNA biosensors lies in the reduced accessibility of target molecules to the DNA probes arranged on heterogeneous surfaces, especially when compared to probe-target recognition in homogeneous solutions. To improve the recognition abilities of these heterogeneous surface-confined DNA probes, much effort has been devoted to controlling the surface chemistry, conformation and packing density of the probe molecules, as well as the size and geometry of the surface. In this review, we aim to summarize the recent progress on the improvement of the probetarget recognition properties by introducing DNA nanostructure scaffolds. A range of new strategies have proven to provide a significantly enhanced range in the spatial positioning and the accessibility of the probes to the surface over previously reported linear structures. We will also describe the applications of DNA nanostructure scaffold-based biosensors. INTRODUCTIONDetection of nucleic acids (DNA or RNA) is an integral step in many applications, including in clinical diagnosis, environmental monitoring and antibioterrorism. 1 With these applications in mind, significant efforts have been made to develop sensitive, selective, rapid and cost-effective DNA (or RNA) biosensors. In parallel, DNAbased devices have also attracted a significant, recent interest owing to their promising applications in nanoelectronics, 2,3 biomolecular computations, 4-8 cellular imaging 9 and drug delivery. [10][11][12] In a typical design of DNA biosensors and nanodevices, oligonucleotides are often used as the molecular recognition layer. Because DNA is comprised of only four bases with A-T and G-C pairing, DNA hybridization processes are highly predictable and can be finely tuned with a rational design of the sequence. In addition, DNA oligonucleotides are usually easy to design, chemically stable and cost-effective.Understanding the physical structure of a DNA probe immobilized on a surface is critical in applications using DNA as a molecular recognition element. The properties of the DNA molecular recognition layer (such as selectivity, sensitivity and reproducibility) highly depend on the structure of the self-assembled DNA film. Modeling studies with thiolated DNA have demonstrated that efficient hybridization occurs in the well-controlled condition of surface-attached probes with large interprobe distances and upright conformations. [13][14][15][16][17][18][19][20][21][22][23][24]

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Section: D-structured Dna Probesmentioning

confidence: 99%

Scaffolded biosensors with designed DNA nanostructures

et al. 2013

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“…The enzyme-catalyzed reduction of H2O2 was electrically coupled to the electrode surface by using 3,3′,5,5′ tetramethylbenzidine (TMB) as electronshuttle. The amperometric current of TMB reduction was proportional with the miRNA concentration in a large concentration range [55]. This detection strategy was reported to enable attomolar detection limits for target miRNAs and discrimination single-base mismatched strands.…”

Section: Electroanalysismentioning

confidence: 99%

“…Wen et al [55] overcame this problem by adapting a base stackingbased strategy to stabilize the sandwich complex. A tetrahedral DNA nanostructure was designed and applied for utmost spatial control of the DNA probe immobilization, which ensured their optimal accessibility.…”

Section: Electroanalysismentioning

confidence: 99%

“…miRNA detection with the tetrahedron-based electrochemical miRNA sensor using enzyme-based signal transduction (either avidin-HRP or high-activity poly-HRP). [55] Reprinted by permission from Macmillan Publishers Ltd: Scientific Reports Ref. [55], copyright (2012).…”

Section: Electroanalysismentioning

confidence: 99%

“…[55] Reprinted by permission from Macmillan Publishers Ltd: Scientific Reports Ref. [55], copyright (2012).…”

Section: Electroanalysismentioning

confidence: 99%

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Electrochemical Detection of miRNAs

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2014

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The recent progress made in the development of electrochemical methods for microRNA (miRNA) detection is presented. This progress is conceived to be largely due to the invention of novel assay methodologies and the use of various bioreagents and nanostructures. These enable a rigorous control over the sensing interface, provide enormous signal amplifications and single-base mismatch specificity. Femtomolar or even subfemtomolar detection limits were shown to be feasible by electrochemical assays. Thus electrochemical detection methodologies are of perspective for diagnostic miRNA detection.

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Construction of Functional DNA Nanostructures for Theranostic Applications

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DNA Nanostructure-based Interfacial engineering for PCR-free ultrasensitive electrochemical analysis of microRNA

Cited by 196 publications

References 45 publications

Scaffolded biosensors with designed DNA nanostructures

Scaffolded biosensors with designed DNA nanostructures

Electrochemical Detection of miRNAs

Construction of Functional DNA Nanostructures for Theranostic Applications

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