DNA Microarrays

Bier, Frank F.; Nickisch‐Rosenegk, Markus von; Ehrentreich‐Förster, Eva; Reiß, Edda; Henkel, Joerg; Strehlow, Rothin; Andresen, Dennie

doi:10.1007/10_2007_087

Cited by 36 publications

(27 citation statements)

References 31 publications

(43 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Genotyping arrays also called SNP chips are devices allowing the simultaneous determination of the genotypes of 10 4 to 2 Â 10 6 SNPs [Bier et al, 2008]. Genotyping array technology has been available for years and is technically mature.…”

Section: Genotyping Arraysmentioning

confidence: 99%

Novel genomic techniques open new avenues in the analysis of monogenic disorders

Kuhlenbäumer¹,

Hullmann²,

Appenzeller³

2011

Hum. Mutat.

103

View full text Add to dashboard Cite

The molecular genetic cause of over 3,000 monogenic disorders is currently unknown. This review discusses how novel genomic techniques like Next-Generation DNA Sequencing (NGS) and genotyping arrays open new avenues in the elucidation of genetic defects causing monogenic disorders. They will not only speed up disease gene identification but will enable us to systematically tackle previously intractable monogenic disorders. These are mainly disorders not amenable to classic linkage analysis, for example, due to insufficient family size. Most monogenic diseases are caused by exonic mutations or splice-site mutations changing the amino acid sequence of the affected gene. These mutations can be identified by sequencing of all exons in the human genome (exome sequencing) rendering whole genome sequencing unnecessary in most cases. Genotyping arrays containing 10⁵ -2×10⁶ single nucleotide polymorphisms (SNPs) and nonpolymorphic markers allow highly accurate mapping of genomic deletions and duplications not detectable by exome sequencing, which are the second most common cause of monogenic disorders. However, several hundred rare, previously unknown sequence variants affecting the amino acid sequence of the encoded protein are found in the exome of every human individual. Therefore, the main challenge will be the differentiation between the many rare benign variants detected by novel genomic techniques and disease causing mutations.

show abstract

Section: Genotyping Arraysmentioning

confidence: 99%

Novel genomic techniques open new avenues in the analysis of monogenic disorders

Kuhlenbäumer¹,

Hullmann²,

Appenzeller³

2011

Hum. Mutat.

103

View full text Add to dashboard Cite

show abstract

“…Once DNA polymorphism is identified, it may be determined ("genotyped") in the population sample by tens of genotyping techniques which exist in hundreds of modifications. Genotyping techniques have been repeatedly reviewed [9][10][11] . We, therefore, limit ourselves to examples of the "classical" genotyping techniques characterized by relatively low throughput.…”

Section: Possibilities For Assessing Dna Polymorphisms In the Immune mentioning

confidence: 99%

Polymorphisms of the Immune Response Genes:selected Biological, Methodical and Medical Aspects

Kubistova¹,

Mrázek²,

Petřek³

2009

BIOMED PAP

View full text Add to dashboard Cite

Background: Variants of the immune response genes (IRG) are considered a potential source of interindividual differences in both innate and adaptive immune responses. A large number of gene polymorphisms have been reported as alternative forms of the IRG nucleotide sequence with important functional consequences for the structure/expression of immune response molecules. Accordingly, IRG polymorphisms are considered responsible for various monogenic diseases. They may also affect individual predisposition to complex diseases or modify their clinical course. Methods and Results:In this review we define IRG polymorphism including its potential functionality. Common approaches used for the investigation of IRG polymorphisms are next briefly described. We then review current approaches (including genome -wide studies) for assessing the importance of particular IRG variants in the susceptibility to and clinical course of complex diseases. Finally, based on our own experience and on the literature, we illustrate current knowledge of the genetic component of two selected complex diseases (sarcoidosis and coronary artery disease).Conclusions: Despite major advances in genotyping technology and general knowledge of the implications of IRG in the susceptibility to complex diseases, the potential clinical application of these approaches still faces major challenges.

show abstract

“…The main advantage of the microarray method is the possibili ty of performing multiparametric analysis of a sample at minimum consumptions of the analyzed material and reagents. [3][4][5] Glass slides with modified surface for covalent immo bilization of probes are generally used as supports for microarrays. 3, 4 The operation of all bioarrays is based on the principle of molecular recognition, due to which spe cific biological complexes are formed in microarray cells.…”

mentioning

confidence: 99%

“…[3][4][5] Glass slides with modified surface for covalent immo bilization of probes are generally used as supports for microarrays. 3, 4 The operation of all bioarrays is based on the principle of molecular recognition, due to which spe cific biological complexes are formed in microarray cells. A tag is introduced into molecules under study to detect these complexes.…”

mentioning

confidence: 99%

Colorimetric detection of immobilized horseradish peroxidase based on the co-oxidation of benzidine derivatives and 4-chloro-1-naphthol

2011

View full text Add to dashboard Cite

Enzymatic co oxidation of benzidine derivatives and 4 chloro 1 naphthol with hydrogen peroxide was studied for the colorimetric detection of horseradish peroxidase using the DNA microarray on glass surface technology. The co oxidation of o dianisidine and 4 chloro 1 naphthol affords a new product with the intense violet color characterized by a high rate of accumulation, good adsorbability on the glass surface, and stability. This reaction can be used as a detection system for the identification of β lactamase genes on DNA microarrays. The DNA microarray technique using horseradish peroxidase and colorimetric detection is characterized by high sensitivity and reproducibility comparable to the characteristics of the microarrays with fluorescence detection.

show abstract

DNA Microarrays

Cited by 36 publications

References 31 publications

Novel genomic techniques open new avenues in the analysis of monogenic disorders

Novel genomic techniques open new avenues in the analysis of monogenic disorders

Polymorphisms of the Immune Response Genes:selected Biological, Methodical and Medical Aspects

Colorimetric detection of immobilized horseradish peroxidase based on the co-oxidation of benzidine derivatives and 4-chloro-1-naphthol

Contact Info

Product

Resources

About