DNA methyltransferase activity detection based on graphene quantum dots using fluorescence and fluorescence anisotropy

Kermani, Hanie Ahmadzade; Hosseini, Morteza; Dadmehr, Mehdi; Hosseinkhani, Saman; Ganjali, Mohammad Reza

doi:10.1016/j.snb.2016.10.078

Cited by 50 publications

(12 citation statements)

References 53 publications

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“…Optical biosensors are one of the best established sensors for the detection of biomolecules. Kermani et al introduced a biosensing method for the detection of DNA methyltransferase which is crucial for the biological activities [95] as abnormal expression of this enzyme may lead to cancer. As shown in the Fig.…”

Section: Characteristics and Classification Of Graphene And Its Derivmentioning

confidence: 99%

Graphene based sensors

Shahdeo

Roberts

Abbineni

et al. 2020

Comprehensive Analytical Chemistry

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Section: Characteristics and Classification Of Graphene And Its Derivmentioning

confidence: 99%

Graphene based sensors

Shahdeo

Roberts

Abbineni

et al. 2020

Comprehensive Analytical Chemistry

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“…Since DNA methyltransferase (M.SssI) enzyme plays a significant role in several biological processes and its abnormal expression can cause cancer, Kermani and the team introduced a biosensing method for monitoring the activity of this enzyme in human serum [95]. This method was based on DNA-functionalized GQDs for a fluorescence-based assay as shown in Figure 2.…”

Section: Fluorescence-based Gqd Sensorsmentioning

confidence: 99%

Applications of Graphene Quantum Dots in Biomedical Sensors

Mansuriya

Altıntaş

2020

Sensors

177

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Due to the proliferative cancer rates, cardiovascular diseases, neurodegenerative disorders, autoimmune diseases and a plethora of infections across the globe, it is essential to introduce strategies that can rapidly and specifically detect the ultralow concentrations of relevant biomarkers, pathogens, toxins and pharmaceuticals in biological matrices. Considering these pathophysiologies, various research works have become necessary to fabricate biosensors for their early diagnosis and treatment, using nanomaterials like quantum dots (QDs). These nanomaterials effectively ameliorate the sensor performance with respect to their reproducibility, selectivity as well as sensitivity. In particular, graphene quantum dots (GQDs), which are ideally graphene fragments of nanometer size, constitute discrete features such as acting as attractive fluorophores and excellent electro-catalysts owing to their photo-stability, water-solubility, biocompatibility, non-toxicity and lucrativeness that make them favorable candidates for a wide range of novel biomedical applications. Herein, we reviewed about 300 biomedical studies reported over the last five years which entail the state of art as well as some pioneering ideas with respect to the prominent role of GQDs, especially in the development of optical, electrochemical and photoelectrochemical biosensors. Additionally, we outline the ideal properties of GQDs, their eclectic methods of synthesis, and the general principle behind several biosensing techniques.

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“…Fluorescence polarization (FP) or fluorescence anisotropy is a rapid, accurate, quantitative, and sensitive technique in the biochemical research in a broad range of experimental conditions [85,86,87,88,89]. The polarization value has reverberate relevance with the rotation rate of the fluorescently labeled molecule, which depends upon the molecular volume and molecular mass at a fixed solution viscosity and temperature [90].…”

Section: Fluorescence Polarization Assaymentioning

confidence: 99%

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

Mozhgani

Kermani

Norouzi

et al. 2020

Heliyon

Self Cite

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Early detection of retroviruses including human T-cell lymphotropic virus and human immunodeficiency virus in the human body is indispensable to prevent retroviral infection propagation and improve clinical treatment. Until now, diverse techniques have been employed for the early detection of viruses. Traditional methods are timeconsuming, resource-intensive, and laborious performing. Therefore, designing and constructing a selective and sensitive diagnosis system to detect serious diseases is highly demanded. Genetic detection with high sensitivity has striking significance for the early detection and remedy of disparate pathogenic diseases. The nucleic acid biosensors are based on the identification of specific DNA sequences in biological samples. Nanotechnology has an important impact on the development of sensitive biosensors. Different kinds of nanomaterials include nanoparticles, nanoclusters, quantum dots, carbon nanotubes, nanocomposites, etc., with different properties have been used to improve the performance of biosensors. Recently, DNA nanobiosensors are developed to provide simple, fast, selective, low-cost, and sensitive detection of infectious diseases. In this paper, the research progresses of nano genosensors for the detection of HIV-1 and HTLV-1 viruses, based on electrochemical, optical, and photoelectrochemical platforms are overviewed.

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DNA methyltransferase activity detection based on graphene quantum dots using fluorescence and fluorescence anisotropy

Cited by 50 publications

References 53 publications

Graphene based sensors

Graphene based sensors

Applications of Graphene Quantum Dots in Biomedical Sensors

Nanotechnology based strategies for HIV-1 and HTLV-1 retroviruses gene detection

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