DNA methylation profile at a satellite region is associated with aberrant placentation in cloned calves

Silveira, Márcia Marques; Bayão, Henrique Xavier Salgado; Borges, Naiara Araújo; Vargas, Luna Nascimento; Caetano, Alexandre Rodrigues; Rumpf, R.; Franco, M. M.

doi:10.1016/j.placenta.2018.08.007

Cited by 18 publications

(15 citation statements)

References 48 publications

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“…Previous studies indicated that epigenetic modifications were altered in cloned abnormal animals compared to naturally bred ones. Global hypo-acetylation, reduced DNA methylation in satellite I region, loss of imprinting (LOS), reduced histone methylation were reported in the somatic cell nuclear transfer derived animals [29,30,31]. Piglets derived by SCNT also showed a higher incidence of malformations and a higher death rate during the perinatal period [32].…”

Section: Discussionmentioning

confidence: 99%

Study on Hematological and Biochemical Characters of Cloned Duroc Pigs and Their Progeny

Shi

Luo

et al. 2019

Animals

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Simple SummaryCloning is the most promising technique for passing the excellent phenotypes of the best individuals in the population. Here we studied the effects of cloning on Duroc pig, which is the most popular sire used in pig production due to its good growth and meat quality. Understanding the changes of cloned Duroc pigs and their progenies is of great importance for animal breeding and public acceptance. The results of this study suggested that there were no difference in blood parameters between the cloned Duroc and the conventionally bred Duroc and their progenies.AbstractTo increase public understanding in cloned animals produced by somatic cell nuclear transfer technology, our previous study investigated the carcass trait and meat quality of the clones (paper accepted), and this study we further evaluate differences by investigating the blood parameters in cloned pigs and their progeny. We collected blood samples from the clones and conventionally bred non-clones and their progeny, and investigated their hematological and blood biochemical characters. Our results supported the hypothesis that there was no significant difference between clones and non-clones, or their progeny. Taken together, the data demonstrated that the clones or their progeny were similar with their controls in terms of blood parameters, although there were still other kinds of disorders, such as abnormal DNA methylation or histone modifications that needs further investigation. The data in this study agreed that cloning technique could be used to preserve and enlarge the genetics of the superior boars in pig breeding industry, especially in facing of the deadly threat of African Swine fever happened in China.

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Section: Discussionmentioning

confidence: 99%

Study on Hematological and Biochemical Characters of Cloned Duroc Pigs and Their Progeny

Shi

Luo

et al. 2019

Animals

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“…Plasmid DNA was isolated using a miniprep protocol described in Sambrook and Russell (). Individual clones were sequenced and analysed as described by Silveira et al () . DNA sequences were compared with GenBank KC753464.1 for XIST and AY044827 for ICR/H19 .…”

Section: Methodsmentioning

confidence: 99%

The dynamics of gene expression, lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows

Braga

Silva

Marques

et al. 2019

Reprod Domestic Animals

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This study aimed to characterize the gene expression, lipid composition and DNA methylation reprogramming during in vitro maturation (IVM) of pig oocytes with different developmental competencies. We used prepubertal gilts and cycling sows as a model to obtain oocytes with different levels of competency. We found that genes involved in lipid metabolism, SLC27A4, CPT2 and PLIN2, and DNA methylation, DNMT3A, TET1 and TET3, possessed altered transcript expression levels during IVM. Specifically, SLC27A4 mRNA (p = 0.05) increased in oocytes from cycling females, whereas CPT2 (p = 0.05), PLIN2 (p = 0.02) and DNMT3A (p = 0.02) increased in oocytes from prepubertal females during IVM. Additionally, TET3 mRNA increased during IVM in oocytes from prepubertal (p = 0.0005) and cycling females (p = 0.02).The TET1 transcript decreased (p = 0.05) during IVM in oocytes from cycling sows.Regarding lipid composition, mass spectrometry revealed a cluster of ions, with molecular masses higher than m/z 700, which comprises a group of complex phospholipids, was identified in all groups of oocytes, except in those from prepubertal gilts.With respect to DNA methylation reprogramming, it was noted that the less competent oocytes were not able to reprogramme the XIST gene during IVM. We conclude that the maternal mRNA store, lipid composition and epigenetic reprogramming are still being established during maturation and are related to oocyte competence. In addition, we propose that the methylation pattern of the XIST may be used as molecular marker for oocyte competence in pigs. K E Y W O R D S Epigenetics, gene expression, molecular markers, oocyte competence, pig How to cite this article: Braga TF, Silva TCF, Marques MG, et al. The dynamics of gene expression, lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows.

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“…However, the process involved in this offset mechanism remains elusive. The DNA hypermethylation of cloned embryos is one of the major failures of proper chromatin remodeling during nuclear reprogramming 42 , 43 , however, in contrast to changes at a global level, severe demethylation in important regions as satellite I and imprinted genes 42 , 43 could also be responsible for such failure.…”

Section: Discussionmentioning

confidence: 99%

Catalytic inhibition of H3K9me2 writers disturbs epigenetic marks during bovine nuclear reprogramming

Sampaio

Sangalli

et al. 2020

Sci Rep

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Orchestrated events, including extensive changes in epigenetic marks, allow a somatic nucleus to become totipotent after transfer into an oocyte, a process termed nuclear reprogramming. Recently, several strategies have been applied in order to improve reprogramming efficiency, mainly focused on removing repressive epigenetic marks such as histone methylation from the somatic nucleus. Herein we used the specific and non-toxic chemical probe UNC0638 to inhibit the catalytic activity of the histone methyltransferases EHMT1 and EHMT2. Either the donor cell (before reconstruction) or the early embryo was exposed to the probe to assess its effect on developmental rates and epigenetic marks. First, we showed that the treatment of bovine fibroblasts with UNC0638 did mitigate the levels of H3K9me2. Moreover, H3K9me2 levels were decreased in cloned embryos regardless of treating either donor cells or early embryos with UNC0638. Additional epigenetic marks such as H3K9me3, 5mC, and 5hmC were also affected by the UNC0638 treatment. Therefore, the use of UNC0638 did diminish the levels of H3K9me2 and H3K9me3 in SCNT-derived blastocysts, but this was unable to improve their preimplantation development. These results indicate that the specific reduction of H3K9me2 by inhibiting EHMT1/2 during nuclear reprogramming impacts the levels of H3K9me3, 5mC, and 5hmC in preimplantation bovine embryos.

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DNA methylation profile at a satellite region is associated with aberrant placentation in cloned calves

Cited by 18 publications

References 48 publications

Study on Hematological and Biochemical Characters of Cloned Duroc Pigs and Their Progeny

Study on Hematological and Biochemical Characters of Cloned Duroc Pigs and Their Progeny

The dynamics of gene expression, lipid composition and DNA methylation reprogramming are different during in vitro maturation of pig oocytes obtained from prepubertal gilts and cycling sows

Catalytic inhibition of H3K9me2 writers disturbs epigenetic marks during bovine nuclear reprogramming

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