DNA Methylation of MMP9 Is Associated with High Levels of MMP-9 Messenger RNA in Periapical Inflammatory Lesions

Campos, Kelma; Gomes, Carolina Cavaliéri; Farias, Lucyana Conceição; Silva, Renato; Letra, Ariadne; Gomez, Ricardo Santiago

doi:10.1016/j.joen.2015.10.002

Cited by 26 publications

(30 citation statements)

References 36 publications

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“…Similar conditions were also found in other diseases. Campos et al found that [13] in periapicalitis, the decreasing extent of methylation of the MMP-9 promoter was consistent with the increasing extent of transcription of MMP-9 mRNA. Roach et al found that [4] the high expression of MMP-9 was associated with the low methylation level of the MMP-9 gene.…”

Section: Discussionmentioning

confidence: 88%

Badanie zależności między metylacją regionu promotora genu MMP-9 a nefropatią cukrzycową

Yang¹,

Feng²,

Yu³

et al. 2018

Endokrynologia Polska

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Introduction: This study aims to explore the relationship between the methylation of matrix metalloproteinase (MMP)-9 gene promoter region and diabetic nephropathy (DN) through the detection of the methylation level of MMP-9 gene promoter region in the peripheral blood of patients with DN in different periods and serum MMP-9 concentration. Material and methods: The methylation level of the MMP-9 gene promoter region was detected by methylation-specific polymerase chain reaction (MSP), and the content of MMP-9 in serum was determined by enzyme-linked immunosorbent assay (ELISA). Results: Results of the statistical analysis revealed that serum MMP-9 protein expression levels gradually increased in patients in the simple diabetic group, early diabetic nephropathy group, and clinical diabetic nephropathy group, compared with the control group, and the difference was statistically significant (P < 0.05). Compared with the control group, the methylation levels of MMP-9 gene promoter regions gradually decreased in patients in the simple diabetic group, early diabetic nephropathy group, and clinical diabetic nephropathy group, and the difference was statistically significant (P < 0.05). Furthermore, correlation analysis results indicated that the demethylation levels of the MMP-9 gene promoter region were positively correlated with serum protein levels, urinary albumin-to-creatinine ratio (UACR), urea, and creatinine, and was negatively correlated with GFR. Conclusions:The demethylation of the MMP-9 gene promoter region may be involved in the occurrence and development of diabetic nephropathy by regulating the expression of MMP-9 protein in serum. (Endokrynol Pol 2018; 69 (3): 269-275)

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Section: Discussionmentioning

confidence: 88%

Badanie zależności między metylacją regionu promotora genu MMP-9 a nefropatią cukrzycową

Yang¹,

Feng²,

Yu³

et al. 2018

Endokrynologia Polska

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“…The intricate transcriptional regulation of MMPs involves mechanism as promoter polymorphisms, epigenetic regulation [22], and post-transcriptional processes [23]. MMP2 gene promoter hypomethylation (and mRNA overexpression) had been previously described in periapical granulomas [24], but this is the first description in bladder cancer. DNA methylation is a covalent modification of the genome, which plays a key role in regulating chromatin structure but also recruit remodeling enzymes to reposition nucleosomes, thereby modulating processes, such as transcription, DNA repair, replication and recombination [25, 26].…”

Section: Discussionmentioning

confidence: 99%

Angiogenin contributes to bladder cancer tumorigenesis by DNMT3b-mediated MMP2 activation

et al. 2016

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Epigenetic-mediated gene activation/silencing plays a crucial role in human tumorigenesis. Eliciting the underlying mechanism behind certain epigenetic changes is essential for understanding tumor biology. Previous studies in human cancers revealed an unrecognized interplay between Angiogenin (ANG) and matrix metalloproteinase-2 (MMP2) leading to pronounced tumorigenesis. Here we provide multiple lines of evidence further indicating ANG oncogenic potential. ANG expression resulted in the hypomethylated state of the MMP2 gene, which led to increased gene expression of MMP2. More than that, our global DNA methylation microarray analysis showed that gene manipulation of ANG affected a variety of pathways, such as cell migration, angiogenesis and specifically, tumor suppressor genes. Mechanistically, ANG negatively regulated DNA methyltransferase 3b (DNMT3b) enzymatic activity by down-regulating its expression and inhibiting its recruitment to the MMP2 promoter. Consistent with this, ANG-MMP2 overexpression and DNMT3b underexpression correlated with reduction in disease free survival of human bladder cancer patients. Together, the results continue to establish ANG as an oncoprotein and further reveal that ANG contributes to oncogenesis by the activation of MMP2 through modulation of DNMT3b functions.

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“…DNA methylation levels of the MMP-2 gene in the peripheral blood of patients with stroke are lower than in controls. High MMP-9 mRNA expression and low DNA methylated profiles of the MMP-9 gene have been found in periapical inflammatory lesions [155]. This indicates a critical role for DNA methylation in periapical inflammation.…”

Section: Epigenetic Mechanismsmentioning

confidence: 99%

Matrix Metalloproteinase Gene Activation Resulting from Disordred Epigenetic Mechanisms in Rheumatoid Arthritis

Araki

Mimura

2017

IJMS

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Matrix metalloproteinases (MMPs) are implicated in the degradation of extracellular matrix (ECM). Rheumatoid arthritis (RA) synovial fibroblasts (SFs) produce matrix-degrading enzymes, including MMPs, which facilitate cartilage destruction in the affected joints in RA. Epigenetic mechanisms contribute to change in the chromatin state, resulting in an alteration of gene transcription. Recently, MMP gene activation has been shown to be caused in RASFs by the dysregulation of epigenetic changes, such as histone modifications, DNA methylation, and microRNA (miRNA) signaling. In this paper, we review the role of MMPs in the pathogenesis of RA as well as the disordered epigenetic mechanisms regulating MMP gene activation in RASFs.

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DNA Methylation of MMP9 Is Associated with High Levels of MMP-9 Messenger RNA in Periapical Inflammatory Lesions

Cited by 26 publications

References 36 publications

Badanie zależności między metylacją regionu promotora genu MMP-9 a nefropatią cukrzycową

Badanie zależności między metylacją regionu promotora genu MMP-9 a nefropatią cukrzycową

Angiogenin contributes to bladder cancer tumorigenesis by DNMT3b-mediated MMP2 activation

Matrix Metalloproteinase Gene Activation Resulting from Disordred Epigenetic Mechanisms in Rheumatoid Arthritis

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