Badanie zależności między metylacją regionu promotora genu MMP-9 a nefropatią cukrzycową

Abstract

Introduction: This study aims to explore the relationship between the methylation of matrix metalloproteinase (MMP)-9 gene promoter region and diabetic nephropathy (DN) through the detection of the methylation level of MMP-9 gene promoter region in the peripheral blood of patients with DN in different periods and serum MMP-9 concentration. Material and methods: The methylation level of the MMP-9 gene promoter region was detected by methylation-specific polymerase chain reaction (MSP), and the content of MMP-9 in serum was determined by enzyme-linked immunosorbent assay (ELISA). Results: Results of the statistical analysis revealed that serum MMP-9 protein expression levels gradually increased in patients in the simple diabetic group, early diabetic nephropathy group, and clinical diabetic nephropathy group, compared with the control group, and the difference was statistically significant (P < 0.05). Compared with the control group, the methylation levels of MMP-9 gene promoter regions gradually decreased in patients in the simple diabetic group, early diabetic nephropathy group, and clinical diabetic nephropathy group, and the difference was statistically significant (P < 0.05). Furthermore, correlation analysis results indicated that the demethylation levels of the MMP-9 gene promoter region were positively correlated with serum protein levels, urinary albumin-to-creatinine ratio (UACR), urea, and creatinine, and was negatively correlated with GFR. Conclusions:The demethylation of the MMP-9 gene promoter region may be involved in the occurrence and development of diabetic nephropathy by regulating the expression of MMP-9 protein in serum. (Endokrynol Pol 2018; 69 (3): 269-275)

The implication of miR-200a and miR-132 expression and their correlations with NF-κB/TNF-alpha signaling in adults with diabetic nephropathy

“…The kidney's matrix metalloproteinases (MMPs), a class of endopeptidases, that are zinc-dependent, possessing comparable biochemical characteristics, are connected to both regular physiological alterations and irregular pathological disorders ( Yang et al, 2018 ). MMP-9 might be crucial for leukocyte migration and regional extracellular matrix proteolysis.…”

Epigallocatechin Gallate Induces the Demethylation of Actinin Alpha 4 to Inhibit Diabetic Nephropathy Renal Fibrosis via the NF-KB Signaling Pathway In Vitro

“…DNA methylation is one of the most common epigenetic changes, and numerous studies have shown that gene hypermethylation promotes the occurrence of DN. [4][5][6][7] The number of differentially methylated regions (DMRs) in DN is significantly higher than that in diabetes without nephropathy. 8 Therefore, reducing the hypermethylation status represents a novel approach for DN treatment.…”