DNA methylation microarray uncovers a permissive methylome for cardiomyocyte differentiation in human mesenchymal stem cells

Govarthanan, Kavitha; Gupta, Piyush Kumar; Ramasamy, Deepa P.; Kumar, Pavitra; Shobana, Mahadevan; Verma, Rama Shanker

doi:10.1016/j.ygeno.2019.08.007

Cited by 17 publications

(8 citation statements)

References 44 publications

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“…By covalently binding to DNA methyltransferase, 5-AzaC reduces DNA methyltransferase activity, thereby reducing the methylation level and inducing cell proliferation and differentiation. Several studies have shown that 5-AzaC could induce differentiation of different types of stem cells into cardiomyocytes [ 4 , 8 , 9 ]. Furthermore, these differentiated cells exhibit spontaneous pulsation and measurable action potentials, consistent with cardiomyocytes [ 4 , 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells

Sun

Zhu

et al. 2020

Stem Cells International

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The use of stem cells in generating cell-based pacemaker therapies for bradyarrhythmia is currently being considered. Due to the propensity of stem cells to form tumors, as well as ethical issues surrounding their use, the seed cells used in cardiac biological pacemakers have limitations. Very small embryonic-like stem cells (VSELs) are a unique and rare adult stem cell population, which have the same structural, genetic, biochemical, and functional characteristics as embryonic stem cells without the ethical controversy. In this study, we investigated the ability of rat bone marrow- (BM-) derived VSELs to differentiate in vitro into cardiomyocytes by 5-Azacytidine (5-AzaC) treatment. The morphology of VSELs treated with 10 μM 5-AzaC increased in volume and gradually changed to cardiomyocyte-like morphology without massive cell death. Additionally, mRNA expression of the cardiomyocyte markers cardiac troponin-T (cTnT) and α-sarcomeric actin (α-actin) was significantly upregulated after 5-AzaC treatment. Conversely, stem cell markers such as Nanog, Oct-4, and Sox2 were continuously downregulated posttreatment. On day 14 post-5-AzaC treatment, the positive expression rates of cTnT and α-actin were 18.41±1.51% and 19.43±0.51%, respectively. Taken together, our results showed that rat BM-VSELs have the ability to differentiate into cardiomyocytes in vitro. These findings suggest that VSELs would be useful as seed cells in exploring the mechanism of biological pacemaker activity.

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Section: Introductionmentioning

confidence: 99%

5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells

Sun

Zhu

et al. 2020

Stem Cells International

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“…The use of fluorescent dyes (Cy3, Cy5, FITC, Alexa fluor 647, Alexa fluor 555, and Alexa fluor 488), radioisotopes ( 32 P, 33 P, and 14 C), near-infrared dyes (phthalocyanine dyes, polymethine carbocyanine dyes, IRDye 800CW, IRDye 750, and IRDye 800), and enzymes (horseradish peroxidase) falls under the category of label-dependent. Importantly, fluorescent dyes (Cy3 label, Cy5,FITC, Alexa flour 647, Alexa fluor 555, Alexa 488, CF555 or CF647, DyLight ® 650, DyLight ® 550 [ 69 ], and Green 540 [ 95 ]) were preferred in most of the microarray articles [ 46 , 49 , 54 , 56 , 57 , 58 , 61 , 65 , 71 , 72 , 73 , 77 , 82 , 83 , 84 , 93 , 97 , 134 , 135 , 136 ]. The reason for this could be their ease of availability, the established protocol to conjugate the dyes, their use for designing a multiplex assay, and the commercially available screening instrumentation (confocal microscope, LuxScan 10K, and GenePix pro 6).…”

Section: Microarray Fabrication Biomolecule Immobilization and Detect...mentioning

confidence: 99%

“…A circular RNA microarray-based [ 101 ] functional experiment revealed cicRNA 7079 as a new antiapoptotic molecule in traumatic spinal cord injuries in mice. The differential DNA methylation profiles [ 136 ] in Wharton’s jelly mesenchymal stem cells (WJ-MSCs), 5′Azacytidine treated WJ-MSC, and human cardiac tissue were studied using a customized 180 K human DNA methylation microarray. Catalytic hairpin assembly involves the use of a hairpin-shaped DNA, which, upon interaction with the target single-stranded analyte, leads to fluorescence signal amplification.…”

Section: Application Of Microarraysmentioning

confidence: 99%

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Tetala

2023

Biomolecules

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Microarrays are one of the trailblazing technologies of the last two decades and have displayed their importance in all the associated fields of biology. They are widely explored to screen, identify, and gain insights on the characteristics traits of biomolecules (individually or in complex solutions). A wide variety of biomolecule-based microarrays (DNA microarrays, protein microarrays, glycan microarrays, antibody microarrays, peptide microarrays, and aptamer microarrays) are either commercially available or fabricated in-house by researchers to explore diverse substrates, surface coating, immobilization techniques, and detection strategies. The aim of this review is to explore the development of biomolecule-based microarray applications since 2018 onwards. Here, we have covered a different array of printing strategies, substrate surface modification, biomolecule immobilization strategies, detection techniques, and biomolecule-based microarray applications. The period of 2018–2022 focused on using biomolecule-based microarrays for the identification of biomarkers, detection of viruses, differentiation of multiple pathogens, etc. A few potential future applications of microarrays could be for personalized medicine, vaccine candidate screening, toxin screening, pathogen identification, and posttranslational modifications.

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“…Even though these cells are less immunogenic, they show better reversing ventricular function ability in animal models [56]. Umbilical cord Wharton's jelly-derived MSCs could also be considered a candidate cell type in cardiac tissue engineering, as they were shown to express cardiacspecific genes inherently without any manipulation [57]. Studies demonstrating the use of small molecule inhibitors to reinforce the enhanced differentiation potential of WJ-MSCs also highlighted the abundant supply of cells necessary for transplantation without any invasive procedures [58].…”

Section: Cardiac Stem Cells (Cscs)mentioning

confidence: 99%

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

Sharma

Dash

Govarthanan

et al. 2021

Cells

Self Cite

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Myocardium Infarction (MI) is one of the foremost cardiovascular diseases (CVDs) causing death worldwide, and its case numbers are expected to continuously increase in the coming years. Pharmacological interventions have not been at the forefront in ameliorating MI-related morbidity and mortality. Stem cell-based tissue engineering approaches have been extensively explored for their regenerative potential in the infarcted myocardium. Recent studies on microfluidic devices employing stem cells under laboratory set-up have revealed meticulous events pertaining to the pathophysiology of MI occurring at the infarcted site. This discovery also underpins the appropriate conditions in the niche for differentiating stem cells into mature cardiomyocyte-like cells and leads to engineering of the scaffold via mimicking of native cardiac physiological conditions. However, the mode of stem cell-loaded engineered scaffolds delivered to the site of infarction is still a challenging mission, and yet to be translated to the clinical setting. In this review, we have elucidated the various strategies developed using a hydrogel-based system both as encapsulated stem cells and as biocompatible patches loaded with cells and applied at the site of infarction.

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DNA methylation microarray uncovers a permissive methylome for cardiomyocyte differentiation in human mesenchymal stem cells

Cited by 17 publications

References 44 publications

5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells

5-Azacytidine-Induced Cardiomyocyte Differentiation of Very Small Embryonic-Like Stem Cells

Recent Progress in Development and Application of DNA, Protein, Peptide, Glycan, Antibody, and Aptamer Microarrays

Recent Advances in Cardiac Tissue Engineering for the Management of Myocardium Infarction

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