DNA methylation and polyamines in regulation of development of the fungus Mucor rouxii

Cano, Carmen; Herrera-Estrella, Luis; Ruíz-Herrera, José

doi:10.1128/jb.170.12.5946-5948.1988

Cited by 26 publications

(17 citation statements)

References 29 publications

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“…Taking into consideration the respective sizes of the CUP transcript and the restriction fragments obtained by digestion with different enzymes, it may be suggested that several CUP loci exist in the genome of M. rouxii, and from their differential sensitivity to digestion by MspI and HpaII, we can conclude that all of these genes are present in the hypermethylated region(s) which was previously described to be present in the spore genome (10). During stage I of germination, at which no DNA duplication has occurred (11), the DNA regions containing these genes remain hypermethylated, as revealed by the restriction analysis of DNA obtained from isodiametrically grown cells (see also reference 10).…”

Section: Discussionmentioning

confidence: 99%

“…More recently we showed that DNA from Mucor spores, both native or in the isodiametrical stage of growth, was highly methylated and became demethylated during germ tube emergence (10). Demethylation required DNA replication and was blocked by 1,4-diaminobutanone (DAB), a competitive inhibitor of ornithine decarboxylase.…”

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confidence: 99%

“…Azacytidine counteracted the inhibitory effect of DAB. We thus advanced the hypothesis that levels of DNA methylation controlled the process of development through regulation of the expression of genes involved in polarized cell growth (10).…”

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confidence: 99%

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Developmental regulation of CUP gene expression through DNA methylation in Mucor spp

et al. 1992

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Inserts which carried the CUP gene from Saccharomyces cerevisiae or Mucor racemosus were used as hybridization probes to measure the methylation state and expression of the CUP gene from Mucor rouxii at different stages of growth. It was observed that the fungus contains a CUP multigene family. All the CUP genes were present in a hypermethylated DNA region in nongrowing and isodiametrically growing spores and were not transcribed at these stages. After germ tube emergence, CUP genes became demethylated and transcriptionally active. Development, demethylation, and transcription of CUP genes were blocked by the ornithine decarboxylase inhibitor 1,4-diaminobutanone. These results suggest that genes that are activated during development become demethylated in this fungus.The idea that DNA methylation regulates gene expression during the development of different eucaryotic organisms has been gaining ground recently (see references 14 and 26 for reviews). Different but significant levels of 5-methylcytosine have been detected in DNA from several species of fungi (2,23,29,30

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Section: Discussionmentioning

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Developmental regulation of CUP gene expression through DNA methylation in Mucor spp

et al. 1992

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“…Fax: (52) (462) 45849/45846. and selectively expressed genes [13], and its reversal by the DNA-methylation inhibitor 5-methyl cytosine [9,11,12], we have suggested that polyamines affect the methylation state of DNA [12][13][14]. In many biological systems it has been demonstrated that the state of DNA methylation may regulate gene expression at different developmental stages [15,16].…”

Section: Introductionmentioning

confidence: 99%

“…Addition of ODC inhibitors blocks differentiation but affects growth to a much lesser extent [9][10][11]. Studying the effect of ODC inhibitors on the levels of methylation of total DNA [12] *Corresponding author. Departamento de Ingenieria Gen6tica, Centro de Investigaci6n y de Estudios Avanzados, Unidad Irapuato, Km.…”

Section: Introductionmentioning

confidence: 99%

Selective inhibition of cytosine‐DNA methylases by polyamines

1995

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We have advanced the hypothesis that polyamines affect DNA methylation and thus promote the expression of developmentally controlled genes. We demonstrate that the activity of cytosine-DNA methyltransferases HpaII, HhaI, HaeIII and SssI is inhibited by physiological concentrations of polyamines.On the other hand, activity of the adenine-DNA methyltransferase EcoRI, and restriction enzymes HpaII, HhaI, HaeIII and EcoRI, is insensitive to polyamine concentrations up to 40 mM.Our results indicate that the effect of polyamines on cytosine-DNA methyltranferases is rather selective and suggest a possible mode of action in vivo.

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Metabolism of polyamines and basic amino acids inErwinia amylovora: Application of liquid chromatography/electrospray mass spectrometry to proferrioxamine precursor feeding and inhibition studies

Feistner

1994

Biol. Mass Spectrom.

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Erwinia amylovora, the etiological agent of fire blight, produces a family of proferrioxamine siderophores, which may be essential for the pathogen to establish itself in its hosts. If so, then control of fire blight may perhaps be possible via interference with proferrioxamine biosynthesis. Proof of this hypothesis requires prior knowledge of the corresponding biosynthetic pathways in E. amylovora. As a first step towards understanding proferrioxamine biosynthesis, it was of interest to investigate the ability of the fire blight bacterium to utilize various potential biosynthetic pathways for diamines. Feeding of lysine, ornithine and diaminobutyric acid gave rise to highly elevated levels of cadaverine, putrescine and diaminopropane, respectively, indicating that the corresponding decarboxylase activities are all present in E. amylovora. The conclusion for lysine decarboxylase was confirmed with (15N2)lysine, which was converted to (15N2)cadaverine. Arginine did not increase putrescine levels substantially, but (13C6)arginine nevertheless gave rise to (13C4)putrescine while suppressing excretion of non-labeled putrescine. A serendipitous result of this study was the finding that the growth of E. amylovora can be inhibited with 5-hydroxylysine and 1,4-diamino-2-butanone. The mechanism of inhibition appears complex and is not yet understood. For 5-hydroxylysine, preliminary investigations point to a competitive inhibition of lysine decarboxylase. However, the growth inhibition cannot be reversed by providing cadaverine, the decarboxylation product of lysine.

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DNA methylation and polyamines in regulation of development of the fungus Mucor rouxii

Cited by 26 publications

References 29 publications

Developmental regulation of CUP gene expression through DNA methylation in Mucor spp

Developmental regulation of CUP gene expression through DNA methylation in Mucor spp

Selective inhibition of cytosine‐DNA methylases by polyamines

Metabolism of polyamines and basic amino acids inErwinia amylovora: Application of liquid chromatography/electrospray mass spectrometry to proferrioxamine precursor feeding and inhibition studies

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