Selective inhibition of cytosine‐DNA methylases by polyamines

Ruíz-Herrera, José; Ruíz-Medrano, Roberto; Domínguez, Ángel

doi:10.1016/0014-5793(94)01360-d

Cited by 26 publications

(17 citation statements)

References 28 publications

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“…However, according to Heby et al (1988) and Ruiz-Herrera (1994), methylation alterations could also be associated with changes in the specific relations between the different individual free polyamines (Table 1). High levels of Spd+Spm and low levels of Put related to reproductive ability in radiata pine, as reported in hazelnut (Rey et al 1994), could imply low dSAM contents (Heby 1995;Ruiz-Herrera et al 1995). This is a substrate of Spd synthase, and also a competitive inhibitor of the DNA methyltransferase, which could increase its action, thereby emphasising the degree of DNA methylation, and possibly cause gene silencing (Razin and Riggs 1980).…”

Section: Resultsmentioning

confidence: 91%

“…Owing to the greater ability of long-chain polyamines, compared with Put, to bind to the genomic DNA (Panagiotidis et al 1995;Pelta et al 1996), the increase in these polyamines causes conformational changes in DNA, exposing certain areas to methylation enzymes (Tabor and Tabor 1984;Smith 1985). Furthermore, a decrease in the Put/(Spd+Spm) ratio coincides with low levels of decarboxylated S-adenosylmethionine (dSAM) (Ruiz-Herrera 1994;Heby 1995;Ruiz-Herrera et al 1995;Heby et al 1988) which is a competitive inhibitor of the DNA methyltransferase. This consequently provokes an increase in the degree of methylation of the genomic DNA.…”

Section: Introductionmentioning

confidence: 96%

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Phase-change related epigenetic and physiological changes in Pinus radiata D. Don

Fraga

Cañal

Rodrı́guez

2002

Planta

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DNA methylation and polyamine levels were analysed before and after Pinus radiata D. Don. phase change in order to identify possible molecular and physiological phase markers. Juvenile individuals (without reproductive ability) were characterised by a degree of DNA methylation of 30-35% and a ratio of free polyamines to perchloric acid-soluble polyamine conjugates greater than 1, while mature trees (with reproductive ability) had 60% 5-methylcytosine and a ratio of free polyamines to perchloric acid-soluble polyamine conjugates of less than 1. Results obtained with trees that attained reproductive capacity during the experimental period confirmed that changes in the degree of DNA methylation and polyamine concentrations found among juvenile and mature states come about immediately after the phase change. We suggest that both indicators may be associated with the loss of morphogenic ability during ageing, particularly after phase change, through a number of molecular interactions, which are subsequently discussed.

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Section: Resultsmentioning

confidence: 91%

Section: Introductionmentioning

confidence: 96%

Phase-change related epigenetic and physiological changes in Pinus radiata D. Don

Fraga

Cañal

Rodrı́guez

2002

Planta

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“…Impairment of movement or of the dissociation of chromatin-associating proteins from chromosomal DNA due to lacking polyamines could impede transcription, recombination and repair, and lead to chromosomal instability and an increased rate of mutation. Specific interactions of polyamines with DNA that imply chromatin configuration are also suggested by the observation that DNA methyltransferases are inhibited by polyamines [112]. However, the fact that Chinese hamster ovary (CHO) cells exposed to DFMO had no significant effect on the condensation state of chromatin during the cell cycle [113] is a caveat against generalisations concerning polyamine pool size changes and functional changes of chromatin.…”

Section: Binding To Anionic Structuresmentioning

confidence: 99%

Polyamines and apoptosis

2005

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Apoptosis designates genetically-programmed mechanisms of cell death. Damage to DNA or to other vital molecules propagates a cascade of reactions, which activate death programs inside the cell [1]. Physiologically apoptosis is an integral part in embryonic development, and the regulation of organ homeostasis. Apoptotic mechanisms are also exploited for tumour therapy [2]. Polyamines and apoptosis AbstractThe natural polyamines putrescine, spermidine and spermine are in multiple ways involved in cell growth and the maintenance of cell viability. In the course of the last 15 years more and more evidence hinted also at roles in gene regulation. It is therefore not surprising that the polyamines are involved in events inherent to genetically programmed cell death. Following inhibition of ornithine decarboxylase, a key step in polyamine biosynthesis, numerous links have been identified between the polyamines and apoptotic pathways. Examples of activation and prevention of apoptosis due to polyamine depletion are known for several cell lines. Elevation of polyamine concentrations may lead to apoptosis or to malignant transformation. These observations are discussed in the present review, together with possible mechanisms of action of the polyamines. Contradictory results and incomplete information blur the picture and complicate interpretation. Since, however, much interest is focussed at present on all aspects of programmed cell death, a considerable progress in the elucidation of polyamine functions in apoptotic signalling pathways is expected, even though enormous difficulties oppose pinpointing specific interactions of the polyamines with pro-and anti-apoptotic factors. Such situation is quite common in polyamine research.

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“…Additionally, some researchers also suggested that polyamines have ability to protect replicating DNA from oxidative damages (Khan et al, 1992;Miyamoto et al, 1993;D'Agustino et al, 2005). It was first reported that polyamines had the role of selective inhibition cytosine-DNA methylases by prevent both the binding and activity of these enzymes (Ruiz-Herrera et al, 1995). Although we have a lot of knowledge about the effects of picloram on non-target organisms, there is no report shown the effect of picloram on DNA methylation and DNA damage (by using the RAPD technique).…”

Section: Introductionmentioning

confidence: 98%

“…Especially, polyamines such as putrescine, spermidine, spermine may establish a correlation with proteins, nucleic acids, membrane phospholipids and cell wall constitutents. Numerous studies claim that polyamines bound to guard DNA from enzymatic degradation, mechanical shearing and X-ray irradiation and also stabilize RNA to counteract of ribosomal dispersion (Miyamoto et al, 1993;Ruiz-Herrera et al, 1995). Additionally, some researchers also suggested that polyamines have ability to protect replicating DNA from oxidative damages (Khan et al, 1992;Miyamoto et al, 1993;D'Agustino et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

Protective Role of Putrescine against Picloram Induced Genomic Instability and DNA Methylation in Phaseolus vulgaris

Taspinar¹,

Arslan²,

Aydın³

et al. 2017

jist

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Picloram (4-amino-3-5-6-trichloropicolinic acid) is an important synthetic auxin and it is one of the most widely used herbicides in agriculture. The use of picloram is representing a potential hazardous to ecosystems and the human health. Putrescine is a kind of polyamine which has a role in plant metabolism such as protecting membrane stability, removing free radicals and nucleic acid and protein synthesis, etc. In this study, DNA damage levels in Phaseolus vulgaris treated with picloram, DNA methylation changes and whether putrescine was any alleviative impact on these alterations were investigated. DNA methylation pattern changes and DNA damage levels were determined by using Randomly Amplified Polymorphic DNA (RAPDs) and Coupled Restriction Enzyme Digestion-Random Amplification (CRED-RAs), respectively. The obtained results indicated that all doses of picloram (5, 10, 20 and 40 mg L -1 ) had a negative effect on RAPDs profile changes (increased DNA damage levels) and decreased of Genomic Template Stability (GTS) and also DNA hypomethylation was seen. However, all concentrations of putrescine (0.01, 0.1 and 1 ppm) reduced these harmful effects of picloram. Consequently, putrescine can be an alternative for reducing genotoxic damage against chemical mutagens in plants.Keywords: DNA methylation, genomic instability, picloram, putrescine ÖZET: Pikloram (4-amino-3-5-6-trikloropikolinik asit) önemli bir sentetik oksin olup, tarımda en çok kullanılan herbisitlerden biridir. Pikloram kullanımı, ekosistem ve insan sağlığı için potansiyel bir tehlike oluşturmaktadır. Bir poliamin çeşidi olan putresinin bitki metabolizmasında; membran stabilitesinin korunması, serbest radikallerin uzaklaştırılması ve nükleik asit ve protein sentezi gibi rolü vardır. Bu çalışma, pikloram uygulanan Phaseolus vulgaris' teki DNA hasar düzeyleri ve DNA metilasyon değişiklikleri ile putresinin bu değişimler üzerinde koruyucu etkisinin olup olmadığının belirlenmesini amaçlamıştır. DNA hasar düzeylerini ve DNA metilasyon değişimlerini belirlemek için Rastgele Çoğaltılmış Polimorfik DNA (RAPD) ve Çift Restriksiyon Enzim KesimiRastgele Çoğaltım (CRED-RA) kullanılmıştır. Sonuçlar, tüm pikloram dozlarının (5, 10, 20 ve 40 mg L -1 ) RAPD profil değişikliklerini (DNA hasarının) artırdığını ve Genomik Kararlılık Stabilitesini (GTS) azalttığını ve ayrıca DNA hipometilasyonunun oluştuğunu göstermiştir. Bununla birlikte, kullanılan tüm putresin konsantrasyonları (0.01, 0.1 ve 1 ppm) putresinin bu zararlı etkilerini azaltmıştır. Sonuç olarak, putresin, bitkilerdeki kimyasal mutajenlere karşı genotoksik hasarı azaltmak için bir alternatif olabilir.

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Selective inhibition of cytosine‐DNA methylases by polyamines

Cited by 26 publications

References 28 publications

Phase-change related epigenetic and physiological changes in Pinus radiata D. Don

Phase-change related epigenetic and physiological changes in Pinus radiata D. Don

Polyamines and apoptosis

Protective Role of Putrescine against Picloram Induced Genomic Instability and DNA Methylation in Phaseolus vulgaris

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