DNA Methylation Analysis in Human Cancer

O’Sullivan, Eileen; Goggins, Michael

doi:10.1007/978-1-62703-287-2_7

Cited by 12 publications

(9 citation statements)

References 105 publications

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“…More than 450,000 methylation sites including promoter region of CpG islands and shores per sample at single nucleotide resolution are provided by this platform (Bibikova et al, 2011;Cheng et al, 2014). The recent advances in high-throughput microarray technologies have enabled investigators to study site-specific DNA methylation events on a much broader scale (O'Sullivan and Goggins, 2013). Many hypermethylation events in CpG islands near known genes transcriptional start sites, which correlated with reductions in gene expression have been identified.…”

Section: Discussionmentioning

confidence: 99%

“…Thus, several methods have been developed recently to analyse genes' methylation status in human cells, depending on their detection strengths and weaknesses (O'Sullivan and Goggins, 2013). One of the methods is the MSPCR which applies specifically designed methylated and unmethylated primers for detecting the methylation level (Herman et al, 1996).…”

Section: Methylation-specific Analysismentioning

confidence: 99%

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Screening of Differential Promoter Hypermethylated Genes in Primary Oral Squamous Cell Carcinoma

Heah

Froemming

Zain

et al. 2014

Asian Pacific Journal of Cancer Prevention

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Background: Promoter hypermethylation leads to altered gene functions and may result in malignant cellular transformation. Thus, identification of biomarkers for hypermethylated genes could be useful for diagnosis, prognosis, and therapeutic treatment of oral squamous cell carcinoma (OSCC). Objectives: To screen hypermethylated genes with a microarray approach and to validate selected hypermethylated genes with the methylation-specific polymerase chain reaction (MSPCR). Materials and Methods: Genome-wide analysis of normal oral mucosa and OSCC tissues was conducted using the Illumina methylation microarray. The specified differential genes were selected and hypermethylation status was further verified with an independent cohort sample of OSCC samples. Candidate genes were screened using microarray assay and run by MSPCR analysis. Results: TP73, PIK3R5, and CELSR3 demonstrated high percentages of differential hypermethylation status. Conclusions: Our microarray screening and MSPCR approaches revealed that the signature candidates of differentially hypermethylated genes may possibly become potential biomarkers which would be useful for diagnostic, prognostic and therapeutic targets of OSCC in the near future.

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Section: Discussionmentioning

confidence: 99%

Section: Methylation-specific Analysismentioning

confidence: 99%

Screening of Differential Promoter Hypermethylated Genes in Primary Oral Squamous Cell Carcinoma

Heah

Froemming

Zain

et al. 2014

Asian Pacific Journal of Cancer Prevention

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“…The development of a cost-effective and easy genotyping platform for identification and selection of desirable epiallele is needed in crop plants. For the identification of epialleles, three major approaches are available and widely used in the case of human (i) bi-sulfite sequencing PCR (BSP), (ii) methylation-specific PCR (MSP), and (iii) Chop-PCR [72,73]. The first two approaches require bi-sulfite conversion of non-methylated cytosine to uracil.…”

Section: Development Of User-friendly Epigenetic Markersmentioning

confidence: 99%

Quantitative Epigenetics: A New Avenue for Crop Improvement

Gahlaut¹,

Zinta²,

Jaiswal³

2020

Preprint

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Plant breeding conventionally depends on genetic variability available in a species to improve a particular trait in the crop. However, epigenetic diversity may provide an additional tier of variation. The recent advent of epigenome technologies has elucidated the role of epigenetic variation in shaping phenotype. Further, the development of epigenetic recombinant inbred lines (epi-RILs) in the model species such as Arabidopsis has enabled accurate genetic analysis of epigenetic variation. Subsequently, mapping of epigenetic quantitative trait loci (epiQTL) allowed association between epialleles and phenotypic traits. Thus, quantitative epigenetics provides ample opportunities to dissect the role of epigenetic variation in trait regulation, which can be eventually utilized in crop improvement programs. Moreover, locus-specific manipulation of DNA methylation by epigenome-editing tools such as clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) can facilitate epigenetic based molecular breeding of important crop plants.

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“…[5] DNA methylation refers to the methylation of DNA cytosine residues at the carbon 5 position. [15,16] DNA methyltransferases (DNMTs) catalyze the transfer of a methyl group to position 5 of a cytosine, generating 5-methylcytosine (5mC). [17] This chemical modification affects gene expression when CpG-rich areas are present inside promoter regions (figure 3).…”

Section: Epigenetic Mechanisms 21 Dna Methylationmentioning

confidence: 99%

Epigenetic Mechanisms in Head and Neck Cancer

Osorio¹,

Castillo²

2016

New Aspects in Molecular and Cellular Mechanisms of Human Carcinogenesis

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Head and neck cancer HNC is one of the most prevalent human malignancies, affecting different anatomic sites of the upper aerodigestive tract U"DT such as the oral cavity, larynx, and naso-, oro-, and hypopharynx. HNC develops through the accumulation of multiple genetic and epigenetic alterations in a multistep process. In this issue, the aim is to describe epigenetic mechanisms behind HNC. The main mechanisms evaluated are DN" methylation, posttranslational histone modification, and noncoding RN"s.

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DNA Methylation Analysis in Human Cancer

Cited by 12 publications

References 105 publications

Screening of Differential Promoter Hypermethylated Genes in Primary Oral Squamous Cell Carcinoma

Screening of Differential Promoter Hypermethylated Genes in Primary Oral Squamous Cell Carcinoma

Quantitative Epigenetics: A New Avenue for Crop Improvement

Epigenetic Mechanisms in Head and Neck Cancer

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