DNA-mediated self-assembly of taste cells and neurons for taste signal transmission

Yun, Junsu; Cho, Ann Na; Cho, Seung Woo; Nam, Yoon Sung

doi:10.1039/c8bm00873f

Cited by 16 publications

(33 citation statements)

References 40 publications

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“…The proximate self-assembly of neuronal and gustatory cells was performed using a pair of complimentary DNA-PEG-lipid conjugates anchored to each type of cells as described in our recent report. 18 The DNA-PEG-lipid conjugates were synthesized by the sequential conjugation of Mal-PEG 24 -NHS to DPPE and single-stranded oligonucleotides (ssODN). The ssODN sequences were (GTCA) 5 and (TGAC) 5 that can be hybridized with each other.…”

Section: Resultsmentioning

confidence: 99%

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Hydrogel Skin-Covered Neurons Self-Assembled with Gustatory Cells for Selective Taste Stimulation

et al. 2019

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Many technical challenges exist in the co-culture of multiple types of cells, including medium optimization, cell-to-cell connection, and selective data acquisition of cellular responses. Particularly, mixed cellular responses limit the precise interpretation of intercellular signal transduction. Here, we report the formation of an agarose gel skin on neurons closely assembled with gustatory cells to selectively stimulate gustatory cells by retarding the diffusion of tastants to neurons. The signal transmission, triggered by denatonium benzoate, from gustatory cells to neurons was monitored using intracellular calcium ion concentrations. The agarose gel skin efficiently suppressed the direct transfer of tastants to neurons, decreasing the number of responsive neurons from 56 to 13% and the number of calcium ion signals per neuron from multiple to single. The assembly of neurons with gustatory cells induced the high level of neuronal responses through taste signal transduction from gustatory cells to neurons. However, the calcium ion signal peaks of free neurons coated with agarose gel were much shorter and weaker than those of neurons closely assembled with gustatory cells. This work demonstrated that agarose gel skin is a simple, fast, and effective means to increase the signal selectivity of cellular responses in the co-culture of multiple types of cells.

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Section: Resultsmentioning

confidence: 99%

“…15−19 A co-culture system mimicking a biological tongue is an essential step to investigate intrinsic cellular responses to tastants and develop a cell-based taste biosensor. 15,17,18…”

Section: Introductionmentioning

confidence: 99%

Hydrogel Skin-Covered Neurons Self-Assembled with Gustatory Cells for Selective Taste Stimulation

et al. 2019

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“…About 6− 8% of astrocytes are known to exist in the murine hippocampus, 34 but the growth of non-neuronal cells was effectively suppressed in the absence of growth factors as previously demonstrated. 26 Cultured neurons exhibited extensive axonal and dendritic arbors. The development of the NeuN-positive nucleus and Tuj1-positive neurites was also previously confirmed.…”

Section: Resultsmentioning

confidence: 99%

“…30−32 Our recent works successfully tracked taste signal transmission between gustatory cells and neurons by generating a DNA-assisted synaptic gap. 26,27 However, the signal frequency and transmission speed were very low due to the limitation of DNA hybridization for cellular assembly. Both gustatory and neuronal cells were modified with a DNA−lipid conjugate, consisting of a single-stranded oligonucleotide (ssODN, 20 bases), poly(ethylene glycol) (PEG), and a phospholipid, as a linker between the cells.…”

Section: Introductionmentioning

confidence: 99%

Artificial Taste Buds: Bioorthogonally Ligated Gustatory–Neuronal Multicellular Hybrids Enabling Intercellular Taste Signal Transmission

Le-Kim

Koo

et al. 2021

ACS Biomater. Sci. Eng.

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Heterogeneous tissue models require the assembly and co-culture of multiple types of cells. Our recent work demonstrated taste signal transmission from gustatory cells to neurons by grafting single-stranded DNA into the cell membrane to construct multicellular assemblies. However, the weak DNA linkage and low grafting density allowed the formation of large gustatory cell self-aggregates that cannot communicate with neurons efficiently. This article presents the construction of artificial taste buds exhibiting active intercellular taste signal transmission through the hybridization of gustatory–neuronal multicellular interfaces using bioorthogonal click chemistry. Hybrid cell clusters were formed by the self-assembly of neonatal gustatory cells displaying tetrazine with a precultured embryonic hippocampal neuronal network displaying trans-cyclooctene. A bitter taste signal transduction was provoked in gustatory cells using denatonium benzoate and transmitted to neurons as monitored by intracellular calcium ion sensing. In the multicellular hybrids, the average number of signal transmissions was five to six peaks per cell, and the signal transmission lasted for ∼5 min with a signal-to-signal gap time of 10–40 s. The frequent and extended intercellular signal transmission suggests that the cell surface modification by the bioorthogonal click chemistry is a promising approach to fabricating functional multicellular hybrid clusters potentially useful for cell-based biosensors, toxicity assays, and tissue regeneration.

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“…Although tricyclic antidepressants [6], clonazepam or diazepam [7] have been proved to be useful for improving the abnormal sensation of taste, it's still lack effective treatment interventions. Most research on taste has focused at the cellular level, revealing the expression of receptors and the transmission of signals [8][9][10]. Meanwhile, since most of the mechanisms that cause dysgeusia are unclear, there is no clear treatment plan for dysgeusia.…”

Section: Introductionmentioning

confidence: 99%

Preparation and application of taste bud organoids in biomedicine towards chemical sensation mechanisms

Liu

Zhu

Tian

et al. 2021

Preprint

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Taste is one of the most basic and important sensations that is able to monitor the food quality and avoid intake of potential danger materials. Whether as an inevitable symptom of aging or a complication of cancer treatment, taste loss so seriously affects the patient’s life quality. Taste bud organoids provide a great convenience for the research of taste functions and the underlying mechanisms due to their characteristics of availability, strong maneuverability, and high similarity to the in-vivo taste buds. This review gives a systemic and comprehensive introduction to the preparation and application of taste bud organoids towards chemical sensing mechanisms. For the first, the basic structure and function of taste buds in biomedicine will be brief introduced. Then, the currently available approaches for the preparation of taste bud organoids are summarized and discussed, which are mainly divided into two categories, i.e. stem/progenitor cell-derived approach and tissue-derived approach. For the next, different applications of taste bud organoids in biomedicine are outlined based on their central roles such as disease modeling, biological sensing, gene regulation, and signal transduction. Finally, the current challenges, future development trends and prospects of research in taste bud organoids are proposed and discussed.

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DNA-mediated self-assembly of taste cells and neurons for taste signal transmission

Abstract: Cells can communicate with one another through physical connections and chemical signaling, activating various signaling pathways that can affect cellular functions and behaviors.

Cited by 16 publications

References 40 publications

Hydrogel Skin-Covered Neurons Self-Assembled with Gustatory Cells for Selective Taste Stimulation

Hydrogel Skin-Covered Neurons Self-Assembled with Gustatory Cells for Selective Taste Stimulation

Artificial Taste Buds: Bioorthogonally Ligated Gustatory–Neuronal Multicellular Hybrids Enabling Intercellular Taste Signal Transmission

Preparation and application of taste bud organoids in biomedicine towards chemical sensation mechanisms

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