DNA markers define two major phylogenetic lineages of Trypanosoma cruzi

Souto, Ricardo Peres do; Fernandes, Octávio; Macedo, Andréa Mara; Campbell, David A.; Zingales, Bianca

doi:10.1016/s0166-6851(96)02755-7

Cited by 502 publications

(441 citation statements)

References 27 publications

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“…Sensitivity and specificity of Stat-Pak were high in Central America, Venezuela and Bolivia, considered to be endemic areas with relatively high prevalence of T. cruzi infection. Although a relatively small number of samples has been analyzed, and most parasites isolated previously from human chronic phase infected individuals were T. cruzi II (Luquetti et al, 1986;Souto et al, 1996), the Chagas Stat-Pak gave comparable results in the different geographical regions studied. Serologic methods are widely accepted for the immunodiagnosis of T. cruzi infection.…”

Section: Discussionmentioning

confidence: 83%

“…T. cruzi is not a homogeneous population of parasites; but consists of a pool of sub-populations that present a high heterogeneity in biologic parameters and genetic characteristics (Souto et al, 1996;Briones et al, 1999;Machado and Ayala, 2001). Furthermore, in a recent consensus meeting, the T. cruzi species were divided into two major phylogenetic lineages: T. cruzi I and T. cruzi II (Satellite Meeting, 1999).…”

Section: Discussionmentioning

confidence: 99%

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Chagas’ disease diagnosis: a multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi

Luquetti

Ponce

et al. 2003

Diagnostic Microbiology and Infectious Disease

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Section: Discussionmentioning

confidence: 83%

Section: Discussionmentioning

confidence: 99%

Chagas’ disease diagnosis: a multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi

Luquetti

Ponce

et al. 2003

Diagnostic Microbiology and Infectious Disease

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“…En T. cruzi se han utilizado diferentes marcadores bioquímicos y moleculares para tratar de correlacionar la diversidad genética del parásito con sus características biológicas. El uso del espaciador intergénico de los genes mini-exón genera dos claras divisiones filogenéticas conocidas como T. cruzi I y T. cruzi II (12), las cuales permiten establecer algunas asociaciones importantes entre la taxonomía y la biología del parásito, el desarrollo de la enfermedad y diferentes parámetros epidemiológicos, entre otros (13,14).…”

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Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

Botero¹,

Mejía²,

Triana³

2007

biomedica

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Introducción. T. cruzi I y T. cruzi II son grupos genéticamente diferentes y se cree que dicha variabilidad es determinante del tropismo tisular en el hospedero vertebrado y responsable de las diversas manifestaciones clínicas de la enfermedad de Chagas. Objetivo. Caracterizar biológica y genéticamente dos clones colombianos de los grupos T. cruzi I y II en el modelo murino. Materiales y métodos. Las cepas CAS15 y AF1 pertenecientes a los grupos T. cruzi I y II fueron clonadas en medio semisólido. Un clon de cada una de ellas y una mezcla de ambos se utilizaron para infectar ratones, los cuales se sacrificaron a diferentes tiempos post-infección. Para analizar la presencia del parásito en sangre y diferentes órganos, se utilizaron el microhematocrito y la reacción en cadena de la polimerasa con los marcadores de la secuencia satélite del ADN nuclear y con el espaciador intergénico del gen mini-exón. Resultados. El clon T. cruzi I fue más infectivo, observándose un tropismo preferencial por corazón, recto y músculo esquelético, mientras que el clon T. cruzi II presentó un tropismo preferencial por bazo e hígado. Durante la infección con la mezcla de los clones, se observó que el clon T. cruzi I predominó sobre el T. cruzi II tanto en sangre como en órganos. Conclusiones. Los resultados confirman que las diferencias genéticas entre los grupos de T. cruzi podrían estar determinando el tropismo tisular y de esta manera jugar un papel fundamental en el entendimiento de las manifestaciones clínicas de la enfermedad de Chagas en Colombia.Palabras claves: Trypanosoma cruzi, enfermedad de Chagas, variación (Genética), tropismo. Biological and genetic characterization of two Colombian clones of Trypanosoma cruzi groups I and IIIntroduction. Genetic differences between T. cruzi I and T. cruzi II may determine differences in their tissue tropism in the vertebrate host and may also be responsible for the differences in clinical manifestations of Chagas disease. Objective. Two Colombian clones of the T. cruzi groups I and II were characterized biologically and genetically in a murine model. Materials and methods. Strains Cas15 and AF1 belonging to the T. cruzi groups I and II were cloned in semisolid medium. A clone of each strain and a mix of both were used to infect mice; the mice were subsequently sacrificed at selected post-infection intervals. In order to identify the parasite presence in blood and organs, two methods were used (a) microhematocrit and (b) polymerase chain reaction with primers for satellite DNA and the intergenic region of miniexon. Results. The T. cruzi I clone was more infectious, with a preferential tropism observed in heart, rectum and skeletal muscle, whereas clone T. cruzi II exhibited a preferential tropism for spleen and liver. During the infection with the clone mixture a predominance of the T. cruzi I clone over clone II in blood as well as in organs was observed. Conclusion. The results corroborate that the genetic differences between the T. cruzi groups correlate with their tissue tropism, an...

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“…This variation has been characterized by the use of isoenzymes (Miles et al 1978, Tibayrenc et al 1986), DNA fingerprinting (Macedo et al 1992), Random Amplified Polymorphic DNA (RAPD; Tibayrenc et al 1993, Gomes et al 1998), Simple Sequence Repeat-anchored PCR (SSR-PCR; Oliveira et al 1997, Gomes et al 1998), Low-stringency Single Specific Primer-PCR (LSSP-PCR; Vago et al 1996) and molecular karyotyping (Henriksson et al 1993). Also, single locus analysis of the ribosomal RNA genes (LSU-rDNA) and mini-exon genes (Souto & Zingales 1993, Souto et al 1996 has suggested the split of T. cruzi strains into three major clusters (1, 2 and 1/2) that possibly represent sub-species (Souto et al 1997).…”

mentioning

confidence: 99%

The population structure of Trypanosoma cruzi: expanded analysis of 54 strains using eight polymorphic CA-repeat microsatellites

Oliveira

Melo

Macedo

et al. 1999

Mem. Inst. Oswaldo Cruz

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DNA markers define two major phylogenetic lineages of Trypanosoma cruzi

Cited by 502 publications

References 27 publications

Chagas’ disease diagnosis: a multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi

Chagas’ disease diagnosis: a multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi

Caracterización biológica y genética de dos clones pertenecientes a los grupos I y II de Trypanosoma cruzi de Colombia

The population structure of Trypanosoma cruzi: expanded analysis of 54 strains using eight polymorphic CA-repeat microsatellites

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