DNA is present in the pyrenoid core of the siphonous green algae of the genusCaulerpa and yellow-green algae of the genusPseudodichotomosiphon

Miyamura, Shinichi; Hori, Takafumi

doi:10.1007/bf01322731

Cited by 15 publications

(7 citation statements)

References 13 publications

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“…Specific interaction of UVI31+ protein via its N-terminal flexible domain with exogenously added pyrenoid fractions (Figure 6 & 7) was observed and is consistent with its localization to pyrenoids in vivo . Recent studies have clearly demonstrated that pyrenoid in C. reinhardtii chloroplasts are associated with processing of RNA stress granules [12] and may also be de novo sites of RNA translation and DNA association [11]. However the functional and physiological implications of these findings are unclear.…”

Section: Discussionmentioning

confidence: 99%

“…Pyrenoids are the sub-organellar structures in the chloroplast of algae, which specialize in carbondioxide concentration and fixation during photosynthesis in the cell. It has been shown that Pyrenoids contain DNA [11] and are also associated with RNA processing in the cell [11], [12]. Further, UVI31+ gets redistributed into punctate foci within the whole chloroplast, away from the pyrenoid, upon exposure to UV.…”

Section: Introductionmentioning

confidence: 99%

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UVI31+ Is a DNA Endonuclease That Dynamically Localizes to Chloroplast Pyrenoids in C. reinhardtii

et al. 2012

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UVI31+ is an evolutionarily conserved BolA family protein. In this study we examine the presence, localization and possible functions of this protein in the context of a unicellular alga, Chlamydomonas reinhardtii. UVI31+ in C. reinhardtii exhibits DNA endonuclease activity and is induced upon UV stress. Further, UVI31+ that normally localizes to the cell wall and pyrenoid regions gets redistributed into punctate foci within the whole chloroplast, away from the pyrenoid, upon UV stress. The observed induction upon UV-stress as well as the endonuclease activity suggests plausible role of this protein in DNA repair. We have also observed that UV31+ is induced in C. reinhardtii grown in dark conditions, whereby the protein localization is enhanced in the pyrenoid. Biomolecular interaction between the purified pyrenoids and UVI31+ studied by NMR demonstrates the involvement of the disordered loop domain of the protein in its interaction.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

UVI31+ Is a DNA Endonuclease That Dynamically Localizes to Chloroplast Pyrenoids in C. reinhardtii

et al. 2012

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“…In contrast to this, scattered chloroplast nucleoids are distributed in some other, relatively minor groups of heterokontophytes, that is, Dictyochophyceae (Moestrup & Thomsen 1990), Eustigmatophyceae (Coleman 1985), Pedinellophyceae (Throndsen 1971;Patterson & Fenchel 1985;Patterson 1986;Koutoulis et al 1988), Pelagophyceae (Andersen et al 1993), Sarcinochrysidales (O'Kelly 1989), and even a species of Xanthophyceae (Miyamura & Hori 1991). In addition to the ring nucleoid, however, Mayama and Shihira-Ishikawa (1994) found DNA-containing dots scattered throughout the chloroplast in Pinnularia nobilis (Ehrenb.)…”

Section: Discussionmentioning

confidence: 98%

Characterization of linear-oblong pyrenoids with cp-DNA along their sides in Nitzschia sigmoidea (Bacillariophyceae)

Mayama

Shihira‐lshikawa

2004

Phycological Res

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SUMMARYIn the past 10 years Nitzschia sigmoidea (Nitzsch) W. Sm. has begun to occur in Japanese rivers in various areas. It is a common diatom in Europe but was previously absent in Japan. Each chloroplast of N. sigmoidea contains many unusual linear-oblong structures. The internal structure of the chloroplast in this species was observed using epifluorescence and electron microscopy with immunolocalization techniques. The linear-oblong structures in the chloroplasts could hardly be observed by conventional light microscopy of living cells, but were obvious in cells stained with propionocarmine. Transmission electron microscopy showed that the cross sections of this structure were lanceolate to fusiform with penetration by a single thylakoid. In cells stained with DAPI, chloroplast DNA was detected along both sides of the linear-oblong structures, and DNA fibrils were detected by electron microscopy. Immunofluorescence microscopy of sectioned cells and also immunoelectron microscopy revealed specific localization of Rubisco between these DNA-containing areas, which divided at the same time as the chloroplast. Our observations confirmed that the linear-oblong structures are pyrenoids. The diversity of localization patterns of chloroplast DNA in diatoms is discussed.

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“…For fluorescence microscopy, cells were fixed with glutaraldehyde (0.3-0.5% final conc.) and stained with 5 μg/ml of 4', 6-diamidino-2-phenylindole (DAPI; Wako Pure Chemical Industries, Osaka, Japan) dissolved in filtered seawater containing a modified S buffer (Miyamura and Hori 1991 …”

Section: /29mentioning

confidence: 99%

Norrisiella sphaerica gen. et sp. nov., a new coccoid chlorarachniophyte from Baja California, Mexico

2007

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DNA is present in the pyrenoid core of the siphonous green algae of the genusCaulerpa and yellow-green algae of the genusPseudodichotomosiphon

Cited by 15 publications

References 13 publications

UVI31+ Is a DNA Endonuclease That Dynamically Localizes to Chloroplast Pyrenoids in C. reinhardtii

UVI31+ Is a DNA Endonuclease That Dynamically Localizes to Chloroplast Pyrenoids in C. reinhardtii

Characterization of linear-oblong pyrenoids with cp-DNA along their sides in Nitzschia sigmoidea (Bacillariophyceae)

Norrisiella sphaerica gen. et sp. nov., a new coccoid chlorarachniophyte from Baja California, Mexico

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