DNA Interaction with PtCl2(LL) (LL = Chelating Diamine Ligand: N,N-Dimethyltrimethylendiamine) Complex

Abstract

The [PtCl(2)(LL)] complex, as a cisplatin derivative, which LL is diamine chelate ligand (N,N-dimethyltrimethylendiamine), was synthesized and characterized by elemental analysis (CHN) mass, (1)H, and (13)C nuclear magnetic resonance techniques. Then the binding of this complex to calf thymus DNA was investigated by various physicochemical methods such as spectrophotometric, circular dichroism, spectrofluorometric, melting temperature, and viscosimetric techniques. Upon addition of the complex, important changes were observed in the characteristic UV-Vis bands (hypochromism) of calf thymus DNA, increase in melting temperature and some changes in specific viscosity. Also, the fluorescence spectral characteristics showed an increase in the fluorescence intensity of methylene blue-DNA solutions in the presence of increasing amounts of metal complex, indicating PtCl(2)(LL) is able to displace the methylene blue bound to DNA but not as complete as intercalative molecules. The experimental results showed that the platinum complex is bound to DNA non-intercalatively, and an outside binding is the preferred mode of interaction.

Some new Ag(I), VO(II) and Pd(II) chelates incorporating tridentate imine ligand: Design, synthesis, structure elucidation, density functional theory calculations for DNA interaction, antimicrobial and anticancer activities and molecular docking studies

“…As expressed previously, the HNAPd metal chelate has a replaceable water ligand in solution, which might be substituted with DNA molecules in solution. [19,35,[67][68][69][70] Likewise because of the expulsion of water ligand from the metal chelate in solution, the explored metal chelates would have a flat part in the middle. Accordingly, conceivable interaction of the HNAPd metal chelate with DNA could be as follows (Scheme 3): first, interaction of Pd(II) metal chelate with base backbone of DNA; second, insertion of the flat part of the metal chelate among the base pairs and thus coordination of Pd 2+ with base pairs of DNA.…”

Synthesis of novel macrocyclic Schiff's‐base and its complexes having N2O2 group of donor atoms. Characterization and anticancer screening are studied

“…The Schiff base 3 and its metal complexes 5 were tested in terms of antibacterial activity using the diffusion agar method. [45][46][47][48][49][50][51][52] The reference compound for antibacterial activities was streptomycin and more than one test organism was used. The antibacterial activity data for compounds 3 and 5 have a wide degree of variation ( Table 5).…”

DNA Interaction and DNA Cleavage Studies of a New Platinum(II) Complex Containing Aliphatic and Aromatic Dinitrogen Ligands

“…We recently investigated the interaction of PtCl 2 (NN) complex (NN = 4,7-dimethyl-1,10 phenanthroline) [49], [PtCl 2 (DIP)] [50] and [PtCl 2 (LL)] in which LL = N,N- Dimethyl-trimethylenediamine [51] with CT-DNA. The addition of solvents other than water, though, ensure solubility of those complexes, increases the hydrophobicity of the bulk solvent, which in turn decreases the DNA binding ability of the complexes.…”