DNA interaction studies of a platinum (II) complex containing an antiviral drug, ribavirin: The effect of metal on DNA binding

Shahabadi, Nahid; kalar, Zeinab Mirzaei; Moghadam, Neda Hosseinpour

doi:10.1016/j.saa.2012.07.020

Cited by 37 publications

(14 citation statements)

References 53 publications

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“…UV-visible absorption spectrum is a basic, most common and simple method, which helps in illustrating the structural changes and also sheds light on complex formation. 33,34 In this study, this method was used to observe the changes of DNA absorbance spectrum in the presence of different concentrations of CS. The absorption spectra of DNA scanned in UV region in the absence and presence of different concentrations of CS (9-150 mg ml À1 ) at pH ¼ 7.4 as shown in Fig.…”

Section: Uv-visible Spectra Observation Of Cs-dna Complexesmentioning

confidence: 99%

Chitosan promotes ROS-mediated apoptosis and S phase cell cycle arrest in triple-negative breast cancer cells: evidence for intercalative interaction with genomic DNA

et al. 2017

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Section: Uv-visible Spectra Observation Of Cs-dna Complexesmentioning

confidence: 99%

Chitosan promotes ROS-mediated apoptosis and S phase cell cycle arrest in triple-negative breast cancer cells: evidence for intercalative interaction with genomic DNA

et al. 2017

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“…As depicted in Fig. Aside from the groove and electrostatic interaction, the stacking interactions of the intercalator with the DNA base pair and helicity stabilization could change the intensities of both hsDNA bands (54,55). After letrozole addition, the intensity of the two absorption bands of hsDNA changed.…”

Section: Spectroscopic Studymentioning

confidence: 97%

“…These phenomena may reflect a shift from a B-like DNA structure toward a structure with some contributions from an A-like conformation (53). Aside from the groove and electrostatic interaction, the stacking interactions of the intercalator with the DNA base pair and helicity stabilization could change the intensities of both hsDNA bands (54,55). Therefore, the interaction between letrozole and hsDNA was predicted to be induced by intercalative mode, which conformed to other experimental results.…”

Section: Spectroscopic Studymentioning

confidence: 99%

Investigation on the interaction of letrozole with herring sperm DNA through spectroscopic and modeling methods

et al. 2015

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The interaction of letrozole, an efficient and safe aromatase inhibitor, with herring sperm DNA (hsDNA) was investigated in vitro through spectroscopy analysis and molecular modeling to elucidate the binding mechanism of anticancer drugs and DNA. The binding constant and the number of binding sites were 2.13 × 10(4) M(-1) and 1.09, respectively, at 298 K. Thermodynamic parameters (ΔG, ΔH and ΔS) exhibited negative values, which indicated that binding was spontaneous and Van der Waals forces and hydrogen bond were the main interaction forces. Fourier transform infrared spectroscopy and other spectroscopy analysis methods illustrated that letrozole could intercalate into the phosphate backbone of hsDNA and interact with the nitrogenous bases. Consistent with the experimental findings, molecular modeling results demonstrated that the interaction was dominated by intercalation and hydrogen bonding. Copyright © 2015 John Wiley & Sons, Ltd.

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“…One approach to accelerate the availability of new drugs is to reposition drugs approved for other indications as anti‐cancer agents . Recently, Sahabadi et al .…”

Section: Introductionmentioning

confidence: 99%

“…Recently, Sahabadi et al . reported the interaction of some anti‐viral drugs with DNA . In this context, we studied the interaction of the anti‐viral drug, EFZ (Fig.…”

Section: Introductionmentioning

confidence: 99%

Binding studies of the anti‐retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques

et al. 2015

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Interactions between efavirenz (EFZ) with calf thymus DNA (CT-DNA) were investigated in vitro under stimulated physiological conditions using multispectroscopic techniques, cyclic voltammetry viscosity measurement, and gel electrophoresis. Methylene blue and acridine orange dyes were used as spectral probes by fluorescence spectroscopy. Hypochromicity was observed in ultra-violet (UV) absorption band of EFZ. Considerable fluorescence enhancement of EFZ was observed in the presence of increasing amounts of DNA solution and the binding constants (Kf ) and corresponding numbers of binding sites (n) were calculated at different temperatures. Thermodynamic parameters including enthalpy change (ΔH) and entropy change (ΔS) were calculated to be -304.78 kJ mol(-1) and -924.52 J mol(-1) K(-1) according to the van 't Hoff equation, which indicated that reaction is predominantly enthalpically driven. In addition, UV/vis absorption titration of DNA bases confirmed that EFZ interacted with guanine and cytosine preferentially. Gel electrophoresis of DNA with EFZ demonstrated that EFZ also has the ability to cleave supercoiled plasmid DNA. Circular dichroism study showed stabilization of the right-handed B form of CT-DNA. All results suggest that EFZ interacts with CT-DNA via an intercalative mode of binding.

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DNA interaction studies of a platinum (II) complex containing an antiviral drug, ribavirin: The effect of metal on DNA binding

Cited by 37 publications

References 53 publications

Chitosan promotes ROS-mediated apoptosis and S phase cell cycle arrest in triple-negative breast cancer cells: evidence for intercalative interaction with genomic DNA

Chitosan promotes ROS-mediated apoptosis and S phase cell cycle arrest in triple-negative breast cancer cells: evidence for intercalative interaction with genomic DNA

Investigation on the interaction of letrozole with herring sperm DNA through spectroscopic and modeling methods

Binding studies of the anti‐retroviral drug, efavirenz to calf thymus DNA using spectroscopic and voltammetric techniques

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