DNA interaction, free radical scavenging andin‐vitroantibacterial activity of drug‐based copper(II) complexes

Abstract

The Cu(II) complexes of type [Cu(cpf)(A n )Cl] (A n = terpyridines, cpf = ciprofloxacin) were synthesized and characterized using IR, mass and reflectance spectra. The free ligands and their complexes were evaluated for their in-vitro antimicrobial activity against a panel of Gram-positive and Gram-negative bacteria. The complexes exhibit better or equal inhibition in comparison to free fluoroquinolones. Binding interactions of the complexes with calf thymus (CT DNA) were investigated by absorption titration, viscosity studies and DNA melting temperature experiment. The cleavage reaction on pUC19 DNA was monitored by agarose gel electrophoresis. The lower concentration of the complexes was catalysed the dismutation of superoxide radical at biological pH, which indicates that the complexes can act as a possible model for superoxide dismutase.

Spectroscopic and electrochemical study for evaluating DNA interaction activity of 4‐(3‐halophenyl)‐6‐(pyridin‐2‐yl)pyrimidin‐2‐amine based piano stool Cp* Rh (III) and Ir (III) complexes

“…Data are presented as ( η / η 0 ) 1/3 versus [complex]/[DNA], where η is the viscosity of DNA in the presence of complex and η 0 is the viscosity of DNA alone. Viscosity values were calculated from the observed flow time of DNA–containing solutions (t) corrected for that of the buffer alone (t 0 ), η = (t‐t 0 ) …”

Synthesis, CMC determination, and intercalative binding interaction with nucleic acid of a surfactant–copper(II) complex with modified phenanthroline ligand (dpq)

“…Quinoxaline and its derivatives are important nitrogen containing heterocyclic compounds of various biologically interesting properties with several pharmaceutical applications. Substituted quinoxalines are an important class of benzoheterocycles, which constitute the building blocks of wide range of pharmacologically active compounds having antibacterial (El-Gendy, El-Meligie, El-Ansary, & Ahmedy, 1995), antifungal (Carta, Sanna, Gherardini, Usai, & Zanetti, 2001;Patel, Dosi, & Bhatt, 2011), and anticancer activities (Sanna, Carta, Loriga, Zanetti, & Sechi, 1999). Some of quinoxaline analogs, such as 2,3-bis(2-pyridyl)-quinoxaline (dpq) complexed with transition metals are of current interest in view of its binding to nucleic acids.…”

Ligational behavior of new mononucleating NOO ethyl pyruvate Schiff base towards 3d metal(II) ions: an emphasis on antiproliferative and photocleavage property

“…In constructing the UV–visible spectrophotometric DNA titration curves, the DNA absorption was subtracted. In order to evaluate quantitatively the DNA‐binding strength, the intrinsic DNA‐binding constant K b was derived by nonlinear regression analysis using equation (): where [DNA] is the concentration of DNA per base pairs, ε a is the apparent absorption coefficient, which was obtained by calculating A abs /[complex], and ε f and ε b are the extinction coefficients for the free complex and complex in the fully bound form, respectively.…”

“…The temperature was scanned from 25 °C to 85 °C at a rate of 5 °C min −1 . The melting temperature ( T m ) was taken as the midpoint of the hyperchromic transition …”