DNA Hybridization at the Air−Water Interface

Abstract

We observed, by using a quartz crystal microbalance, linear oligonucleotides binding selectively to the planar nucleobase lipid monolayer at the air-water interface and hybridization between two linear oligonucleotides in aqueous solution. Kinetics of DNA hybridization at the interface could be controlled by changing the distance and orientation between nucleobases in the monolayer and compared with those for conventional hybridization in the aqueous solution.

DNA Microarrays Based on Noncovalent Oligonucleotide Attachment and Hybridization in Two Dimensions

“…Alternatively, it may be formally possible that structures approximating a ribbon-like, nonhelical duplex may be forming at the surface. Such structures have been reported in different contexts, such as hybridization at air-water interfaces (14). Work is in progress to resolve these potential configurations.…”

Interfacially formed organized planar inorganic, polymeric and composite nanostructures

“…Phase separation effects in amphiphilic block copolymer monolayer at the air-water interface was observed [313]. Using Langmuir monolayer approach, DNA complexes with cationic amphiphiles [133,134,314,315] and nucleolipids [316] were formed at the gas-liquid interface. Using Langmuir monolayer technique and amphiphilic oligonucleotides, DNA hybridization at the air-aqueous phase interface was studied [317].…”

Fourier transform surface-enhanced Raman scattering of single-layer nucleolipid Langmuir–Blodgett films on silver island film substrates

“…Recently, a variety of mimic systems, such as micelles [2][3][4], vesicles [5], and monolayers at the air/water interface [6][7][8][9][10][11][12], have been used to study the complementary binding of the nucleobases. The complementary binding at the air/water interface was first described through a variation of the conventional surface pressure-area (π -A) isotherm by Kitano and Ringsdorf et al [6].…”

“…Subsequent efforts in this research field were directed toward using many analytic approaches for the investigation of the complementary binding. Ultraviolet-visible (UV-vis) [7,8], Fourier transform infrared (FT-IR) [8][9][10], and X-ray photoelectron spectroscopy (XPS) [11] and quartz crystal microbalance (QCM) [12] have been successfully used, whereas Raman spectroscopy has failed [13].…”