DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates

Prévost, Gilles; Jaulhac, B.; Piémont, Y.

doi:10.1128/jcm.30.4.967-973.1992

Cited by 245 publications

(126 citation statements)

References 19 publications

(19 reference statements)

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“…In conclusion, we showed, like others [1,4,23], that PFGE is a very effective typing method. However, it is time-consuming and requires specific equipment.…”

Section: Discussionsupporting

confidence: 77%

Comparison of ribotyping, pulsed-field gel electrophoresis and random amplified polymorphic DNA for typingClostridium difficilestrains

Chachaty¹,

Saulnier²,

Martín³

et al. 1994

FEMS Microbiology Letters

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Clostridium difficile is a Gram-positive sporulating anaerobic bacillus which causes pseudomembranous colitis. Nosocomial acquisition of this bacteria has proved frequent, and epidemiological markers are needed to recognize and control common-source outbreaks. We therefore compared the results of pulsed-field gel electrophoresis (PFGE) after restriction with SmaI or NruI, random-amplified polymorphic DNA (RAPD) using 3 10-mer oligonucleotides, and ribotyping to differentiate between 30 unrelated strains of C. difficile belonging to 8 serotypes. The strains were separated into 26 different types by PFGE, 25 by RAPD, but into only 18 types by ribotyping. Median percentages of similarity between strains ranged from 27 in the PFGE assay to 90 in the ribotyping assay, but there was good agreement between the 3 methods for the clustering of strains. PFGE was more time-consuming than RAPD but its patterns were easier to analyze.

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“…In conclusion, we showed, like others [1,4,23], that PFGE is a very effective typing method. However, it is time-consuming and requires specific equipment.…”

Section: Discussionsupporting

confidence: 77%

Comparison of ribotyping, pulsed-field gel electrophoresis and random amplified polymorphic DNA for typingClostridium difficilestrains

Chachaty¹,

Saulnier²,

Martín³

et al. 1994

FEMS Microbiology Letters

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“…When we considered other markers such as resistance to antimicrobials or biochemical characters, we were able to further discriminate amongst isolates belonging to a clone as defined by MALDI-TOF MS. Thus, combinations of various epidemiological markers further confirm the possibility to access a more acute discrimination amongst bacterial isolates (Pr evost et al 1992;Tenover et al 1997). This work suggests that V. parahaemolyticus isolates can be discriminated without any identification of specific proteins.…”

Section: Vibrio Parahaemolyticus Is a Significant Pathogen In Marine supporting

confidence: 61%

Rapid identification ofVibrio parahaemolyticusisolated from shellfish, sea water and sediments of the Khnifiss lagoon, Morocco, by MALDI-TOF mass spectrometry

Malainine

Moussaoui

Prévost

et al. 2013

Lett Appl Microbiol

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We establish the presence of Vibrio parahaemolyticus and deepen the comparison of isolates using MALDI-TOF MS for the typing of isolates originating from the Khnifiss lagoon (Morocco). Amongst 48 samples from sea water, sediment and shellfish isolated from different sites of Khnifiss lagoon, Morocco, we obtained 22 isolates of V. parahaemolyticus identified by Vitek 2™ System (bioMérieux) and MALDI Biotyper™ (Bruker Daltonics). All isolates were highly resistant to ampicillin and ticarcillin, moderately resistant to cefalotin, but sensitive to 16 other antimicrobials tested. MALDI-TOF MS was used to discriminate between closely related environmental strains of V. parahaemolyticus. A clustering and distribution based on MALDI-TOF spectra were generated using the BioTyper 1.1™ software. Despite low diversity in regard to the biochemical characteristics and antimicrobial resistance, the isolates evoke a larger biodiversity when analysed through mass spectra of abundant proteins. Different evaluations of a cut-off value showed that, when placed at a 10% threshold of the whole diversity, isolates differed by at least three mass peaks.

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“…As four of the canine isolates were from animals presenting at the same veterinary practice, the possibility of cross-infection was considered ( Table 2). However, pulsed-field gel electrophoresis banding patterns [12] indicated that these isolates were not closely related [13].…”

mentioning

confidence: 88%

Characterisation of methicillin-resistant Staphylococcus aureus isolates from dogs and their owners

Boost

O'Donoghue

Siu

2007

Clinical Microbiology and Infection

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Ten methicillin-resistant Staphylococcus aureus (MRSA) isolates from healthy owners and their pets were characterised by susceptibility testing, staphylococcal chromosome cassette (SCC)mec and agr typing, and detection of the Panton-Valentine leukocidin (PVL) genes. Two human and three dog isolates harbouring SCCmec type III appeared to be of hospital origin. The five remaining isolates carried SCCmec type IV, with three being multidrug-resistant. One type IV isolate was PVL-positive and a prototypic agr type 3, typified by strain MW2. This is the first report of this type in association with nasal carriage. Drug resistance may be increasing among community isolates of MRSA.

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DNA fingerprinting by pulsed-field gel electrophoresis is more effective than ribotyping in distinguishing among methicillin-resistant Staphylococcus aureus isolates

Cited by 245 publications

References 19 publications

Comparison of ribotyping, pulsed-field gel electrophoresis and random amplified polymorphic DNA for typingClostridium difficilestrains

Comparison of ribotyping, pulsed-field gel electrophoresis and random amplified polymorphic DNA for typingClostridium difficilestrains

Rapid identification ofVibrio parahaemolyticusisolated from shellfish, sea water and sediments of the Khnifiss lagoon, Morocco, by MALDI-TOF mass spectrometry

Characterisation of methicillin-resistant Staphylococcus aureus isolates from dogs and their owners

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