Comparison of ribotyping, pulsed-field gel electrophoresis and random amplified polymorphic DNA for typing<i>Clostridium difficile</i>strains

Chachaty, Élisabeth; Saulnier, Patrick; Martín, António; Mario, Nathalie; Andremont, Antoine

doi:10.1111/j.1574-6968.1994.tb07144.x

Cited by 54 publications

(41 citation statements)

References 30 publications

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“…1998) where RT and RAPD analysis have generally been found to correlate with each other. However, our results are in agreement with those of Chachaty et al . (1994) who found that, although clusters of Clostridium difficile identified by RT and RAPD analysis were similar, the relative distances between clusters differed for these two methods.…”

Section: Discussionsupporting

confidence: 94%

Use of ribotyping and random amplified polymorphic DNA to differentiate isolates of Burkholderia andropogonis

Bagsic-Opulencia¹,

Hayward²,

Fegan³

2001

J Appl Microbiol

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Aims: The aim of this study was to determine the genetic diversity among isolates of Burkholderia andropogonis from various host plant species and geographic locations. Methods and Results: Both random amplified polymorphic DNA (RAPD) and ribotyping analyses were used to assess the diversity of B. andropogonis isolates and compare these results with pathogenicity assays carried out on a number of common hosts of the organism. Conclusions: Both RAPD and ribotyping analyses revealed a high level of genetic diversity between isolates of B. andropogonis. Both methods demonstrated a similar clustering of isolates. However, there was no strict correlation between the genetic diversity revealed and the original host, geographic location or pathogenicity of the isolates. Significance and Impact of the Study: This is the first report on the genetic diversity of isolates of B. andropogonis. The great degree of diversity revealed in this study contrasts with the lack of phenotypic diversity within this species.

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Section: Discussionsupporting

confidence: 94%

Use of ribotyping and random amplified polymorphic DNA to differentiate isolates of Burkholderia andropogonis

Bagsic-Opulencia¹,

Hayward²,

Fegan³

2001

J Appl Microbiol

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“…expected, RS-PCR fingerprinting was slightly more reproducible than AP-PCR, given the high degree of susceptibility of the latter procedure to variations in testing conditions (9). Nevertheless, the reproducibility of AP-PCR was adequate, and this relatively straightforward technique had a high degree of discrimination.…”

Section: Vol 43 2005 Molecular Epidemiology Of Endemic C Difficilementioning

confidence: 99%

Molecular Epidemiology of EndemicClostridium difficileInfection and the Significance of Subtypes of the United Kingdom Epidemic Strain (PCR Ribotype 1)

et al. 2005

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“…Despite this, accessibility, in addition to time and cost restraints, means that MLVA is still not widely utilized. Random amplification of polymorphic DNA (RAPD) PCR is a quick, cost-effective method which has previously been used to characterize C. difficile isolates (Barbut et al, 1994;Chachaty et al, 1994;van Dijck et al, 1996). The method is often criticized, however, due to lack of reproducibility (Brazier, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genetic characterization of clinical isolates of Clostridium difficile using an optimized RAPD protocol and PCR ribotyping reveals strain diversity between two tertiary referral Trusts in the West Midlands, UK

Green

Worthington

Hilton

et al. 2011

Journal of Medical Microbiology

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Epidemiological investigations of Clostridium difficile often focus on differences between separate geographical areas. In this investigation, two populations of C. difficile recovered from separate tertiary referral Trusts within the West Midlands, UK, were characterized using both PCR ribotyping and an optimized RAPD (random amplification of polymorphic DNA) protocol. The PCR ribotyping and RAPD methodologies identified differences between the two C. difficile populations, in both the prevalence and the diversity of types identified. The use of PCR ribotyping in conjunction with RAPD further categorized different types within defined PCR ribotypes, identifying different types within the same PCR ribotype and therefore providing a greater discriminatory power than either of the methods when used alone. The differences observed in this study between the two Trusts in the distribution of both RAPD 'type' and PCR ribotype demonstrate the diversity that is present amongst isolates of C. difficile within a relatively small geographical area and warrants a need for further investigation into the local epidemiology of C. difficile.

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Comparison of ribotyping, pulsed-field gel electrophoresis and random amplified polymorphic DNA for typingClostridium difficilestrains

Cited by 54 publications

References 30 publications

Use of ribotyping and random amplified polymorphic DNA to differentiate isolates of Burkholderia andropogonis

Use of ribotyping and random amplified polymorphic DNA to differentiate isolates of Burkholderia andropogonis

Molecular Epidemiology of EndemicClostridium difficileInfection and the Significance of Subtypes of the United Kingdom Epidemic Strain (PCR Ribotype 1)

Genetic characterization of clinical isolates of Clostridium difficile using an optimized RAPD protocol and PCR ribotyping reveals strain diversity between two tertiary referral Trusts in the West Midlands, UK

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