DNA damage induction in human cells exposed to vanadium oxides in vitro

Vanadium is an environmental pollutant attached to the smallest air suspended particles that enters into the respiratory tract reaching the systemic circulation. The oxidative state of this element and sex are factors related to its toxicity. In this study, we explored sex-associated genotoxic and cytotoxic differences in a mouse experimental model. Mice inhaled V2O5 (0.02 M) 2 h/twice a week; blood samples were obtained at 24 h and every week until the end of the 4-week exposure. Samples were processed for fluorochrome-mediated viability and a micronucleus assay in slides pre-covered with acridine orange (AO). The results showed that males were more susceptible to genotoxicity during the exposure in contrast to the females. In peripheral blood leukocytes, no cytotoxic differences were observed in both, females or males, but the decrease in circulating reticulocytes provides evidence of the metal's cytotoxic effect on the bone marrow (BM). A significant decrease in reticulocytes was observed during the experiment independent of the animal's sex. The present findings might be explained by the interaction of the metal with the enzymes that control erythropoiesis or a direct effect on erythropoietin production might explain our findings; however, an absence of the genotoxic effects in females could be a consequence of the protective effect against oxidative stress by their higher estrogen levels. This study contributes to a better understanding of the mechanisms by which vanadium induces adverse effects in biological systems.

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“…The dual fluorochrome assay is an indicator of cell metabolism and death caused by cell membrane injury (Rodríguez‐Mercado et al . ).…”

Section: Discussionmentioning

confidence: 97%

“…Information related to genotoxicity and the carcinogenic potential of vanadium pentoxide is still limited and not conclusive (Rodríguez‐Mercado et al . , , ; Boobis et al . ).…”

Section: Introductionmentioning

confidence: 99%

Sex differences in blood genotoxic and cytotoxic effects as a consequence of vanadium inhalation: micronucleus assay evaluation

Rojas-Lemus

Altamirano-Lozano²,

Fortoul

2013

J of Applied Toxicology

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“…Related to Wolff's law , under these circumstances the bone tissue cannot regenerate and the mechanical bond between bone and the implant could be eventually lost . Moreover, vanadium and aluminium have been proven to have a negative immunological influence on an organism . Considering these facts, current materials research focuses on β‐titanium alloys that have lower elastic moduli (approximately 40–80 GPa) than the α and α–β alloys .…”

Section: Introductionmentioning

confidence: 99%

Electrochemical behaviour of the nanostructured surface of Ti‐35Nb‐2Zr alloy for biomedical applications

Fojt

Hybášek

Joska

2016

Materials & Corrosion

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Titanium β‐alloys are perspective material for orthopaedic implants. Their bioactivity could be increased by surface nanostructuring. The nanotubular surface on the Ti‐35Nb‐2Zr alloy was prepared by anodic oxidation. The surfaces were characterised by electron microscopy and photoelectron spectroscopy. The electrochemical behaviour was determined by impedance spectroscopy and Mott–Schottky measurement. The surface analysis indicates enrichment of the oxidic layer by niobium. Nanotubular oxide layer behaviour in phosphate‐buffered solution was compared with native passive oxides surface and with thick oxide layer. The results of impedance measurement on the nanotubes were strongly affected by oxygen in the test solution. The corrosion resistance of the nanostructure was higher than that of the native passive layer. In the terms of the corrosion behaviour, the nanostructuring seems to be a promising surface treatment for medical applications.

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“…It was observed that vanadium(III) trioxide and vanadium(V) pentoxide produced DNA single-strand breaks at all of the concentrations (1, 2, 4, or 8 μg/ml) and treatment times (2,4, or 6 h) tested. The results show that the genotoxic effect of vanadium can be produced by any of its three oxidation states [17]. Cell death and DNA-damage induced by silver nanoparticles (AgNP-P) were prevented by Tiron and dimethyl thiourea that scavenge and H 2 O 2 , respec-…”

Section: Recent In Vitro Assays For Oxidative Dna Damages With Industmentioning

confidence: 97%

Oxidative DNA damages by chemical exposures at work

Rim¹

2012

ABB

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ABSTRACTdiseases. These include cancer, Parkinson's disease, Alzheimer's disease, atherosclerosis, heart failure, myocardial infarction, Schizophrenia, bipolar disorder, fragile X syndrome, sickle cell disease and chronic fatigue syndrome. Oxidative stress is likely to be involved in agerelated development of cancer. The reactive species produced in oxidative stress can cause direct damage to the DNA and are therefore mutagenic, and it may also suppress apoptosis and promote proliferation, invasiveness and metastasis [1].Oxidative DNA damage is an inevitable consequence of cellular metabolism, with a propensity for increased levels following toxic insult. Of the molecules subject to oxidative modification, DNA has received the greatest attention, with biomarkers of exposure and effect closest to validation. There are many chemicals in workplaces that could cause oxidative DNA damages such as carcinogens. This review concentrated on studies published between the years 2000 and 2012 that used to detect 8-oxodG in humans (workers), laboratory animals and in cell lines. Given the recent toxicological results from oxidative stress, it is important to review these studies to improve the current understanding of the oxidative DNA damages by chemical exposures at work. It also suggests that biomarkers may be responsible for understanding the role of oxidative DNA damage in disease, highlighting the need for further studies.

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DNA damage induction in human cells exposed to vanadium oxides in vitro

Cited by 80 publications

References 37 publications

Sex differences in blood genotoxic and cytotoxic effects as a consequence of vanadium inhalation: micronucleus assay evaluation

Sex differences in blood genotoxic and cytotoxic effects as a consequence of vanadium inhalation: micronucleus assay evaluation

Electrochemical behaviour of the nanostructured surface of Ti‐35Nb‐2Zr alloy for biomedical applications

Oxidative DNA damages by chemical exposures at work

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