DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro

Matzenbacher, Cristina Araujo; Garcia, Ana Letícia Hilário; Santos, Marcelo Soares Teles; Nicolau, Caroline Cardoso; Premoli, Suziane; Corrêa, Dione Silva; Souza, Cláudia Telles de; Niekraszewicz, Liana Appel Boufleur; Dias, Johnny Ferraz; Delgado, T.; Kalkreuth, W.; Grivicich, Ivana; Silva, Juliana da

doi:10.1016/j.jhazmat.2016.11.062

Cited by 80 publications

(19 citation statements)

References 49 publications

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“…The report says that nanosize fly ash and PAHs were found to induce DNA damage which was evaluated through comet assay. Nonetheless, our study is in line with other research which states that the coal and its by-product were observed to induce oxidative stress-dependent DNA damage [30].…”

Section: Dna Damagesupporting

confidence: 93%

Identification, Quantification and <i>In-Vitro</i> Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill

Verma¹,

Meena²,

Maurya³

et al. 2019

JEP

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Sugarcane burning during harvest and non-harvest season emits various pollutants like volatile organic compounds (VOCs), alkanes, and PAHs (Polyaromatic hydrocarbons) in the surrounding environment. Among these pollutants, PAHs are of uttermost concern due to their high level of toxicity. Burning of sugarcane bagase in sugar mill results in the production of fly ash. Fly ash is produced as a result of sugarcane bagasse burning in sugar mills. In present study, fly ash that comes out from the sugar mill chimney was collected from Western Uttar Pradesh, India and used for further analysis. High temperature and incomplete combustion inside chimney lead to the formation of PAHs. Extraction of PAHs present in fly ash samples was done by ultrasonication method and was identified with GC-FID (gas chromatography-flame ionization detector). Results exhibit the presence of eight PAHs in fly ash samples where the Benzo(a)pyrene and Naphthalene were found to be in high concentration. Furthermore, we have evaluated toxic effects of fly ash and Polyaromatic hydrocarbons (Standard of BaP & Nap) through different methods i.e. MTT, ROS and comet assay. Significant reduction (p < 0.001) in cell viability was noted in cells treated with fly ash as compared to control. Fly ash samples were also found to induce significant oxidative stress in HeLa cells, which ultimately causes DNA damage. Therefore, it may be concluded that the fly ash samples are toxic to the environment due to the presence of PAHs. Hence, the present study plays an important role in determining the harmful effects of PAHs and their source of occurrence.

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Section: Dna Damagesupporting

confidence: 93%

Identification, Quantification and <i>In-Vitro</i> Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill

Verma¹,

Meena²,

Maurya³

et al. 2019

JEP

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“…2 Matzenbacher et al tried to assess the mutagenic and genotoxic effects of coal dust, fly and bottom ash using lung fibroblast cell line (V79) with the MN test and the comet assay together. 10 Another investigation in human primary parotid gland cells, nicotine derived genotoxic effects were evaluated in vitro by MN test, comet assay and chromosome aberration test at the same time. 11 In one of our studies, we investigated the genotoxic effects of 186Re-1,1-hydrox yethylidenediphosphonate (186Re-HEDP), by using a micronucleus (MN)-fluorescence in-situ hybridization (FISH) assay, on the cultured peripheral blood lymphocytes.…”

Section: Micronucleus Based Genotoxicity Testsmentioning

confidence: 99%

Micronucleus Based Genotoxicity Tests

Baydin¹

2019

Turkiye Klinikleri J Med Sci

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“…1). The presence of PAH was found in its coal, bottom and fly ash samples 29 , metals were detected in soil samples 30 and Zn, Ni, Cu, Cd, V and Pb were detected in the tissues of the subterranean rodent C. torquatus 23 . In addition, studies with organisms have shown DNA damage in plants ( Baccharis trimera ) 9,31 , snail ( Helix aspersa ) 10 and coal workers’ blood 30,32,33 .…”

Section: Introductionmentioning

confidence: 98%

Anthropogenic Effects on Natural Mammalian Populations: Correlation Between Telomere Length and Coal Exposure

Matzenbacher

Silva

Garcia

et al. 2019

Sci Rep

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The Candiota coal mine in Rio Grande do Sul (RS) is one of the largest in Brazil. Coal is a fossil fuel that causes environmental impacts from its extraction to combustion due to the release of different agents, such as polycyclic aromatic hydrocarbons (PAH) and heavy metals. C tenomys torquatus are herbivorous and subterranean rodents that dig tunnels with their paws and teeth and can be exposed to coal through contaminated food. Exposure to pollutants can cause DNA damage and affect different tissues, inducing alterations in the population structure and genetic diversity. Our study aimed to evaluate the effect of exposure to coal and its derivatives on the C. torquatus population and to examine the relationship of coal exposure with variations in absolute telomere length (aTL), global DNA methylation and genotoxicity. Our study showed an inverse correlation between telomere length and coal exposure in addition to an increase in DNA damage. The results indicate that coal and its byproducts can contribute to the alteration of the C. torquatus population structure, as evidenced by a reduction in the number of adults.

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DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro

Cited by 80 publications

References 49 publications

Identification, Quantification and <i>In-Vitro</i> Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill

Identification, Quantification and <i>In-Vitro</i> Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill

Micronucleus Based Genotoxicity Tests

Anthropogenic Effects on Natural Mammalian Populations: Correlation Between Telomere Length and Coal Exposure

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DNA damage induced by coal dust, fly and bottom ash from coal combustion evaluated using the micronucleus test and comet assay in vitro

Cited by 80 publications

References 49 publications

Identification, Quantification and &lt;i&gt;In-Vitro&lt;/i&gt; Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill

Identification, Quantification and &lt;i&gt;In-Vitro&lt;/i&gt; Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill

Micronucleus Based Genotoxicity Tests

Anthropogenic Effects on Natural Mammalian Populations: Correlation Between Telomere Length and Coal Exposure

Contact Info

Product

Resources

About

Identification, Quantification and <i>In-Vitro</i> Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill

Identification, Quantification and <i>In-Vitro</i> Genotoxicity of Major Polyaromatic Hydrocarbons Produced by Sugarcane Fly Ash Emitted from Sugarmill