DNA damage in children with asthma bronchiale and its association with oxidative and antioxidative measurements

Zeyrek, Dost; Çakmak, Alpay; Ataş, Ali; Koçyiğit, Abdürrahim; Erel, Özcan

doi:10.1111/j.1399-3038.2008.00780.x

Cited by 34 publications

(25 citation statements)

References 27 publications

(32 reference statements)

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“…Zeyrek et al [22 ]reported higher TOS and TAS levels and greater DNA damage in a population of pediatric asthma patients than in controls. In our study, TAS levels were decreased compared with the control group, probably because of being used to balance oxidative stress.…”

Section: Discussionmentioning

confidence: 99%

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Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

Dilek

Özkaya²,

Koçyiğit³

et al. 2015

Int Arch Allergy Immunol

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Background: There is ample knowledge reported in the literature about the role of oxidative stress in asthma pathogenesis. It is also known that the interaction of reactive oxygen species with DNA may result in DNA strand breaks. The aim of this study was to investigate if montelukast monotherapy affects oxidative stress and DNA damage parameters in a population of pediatric asthma patients. Methods: Group I consisted of 31 newly diagnosed asthmatic patients not taking any medication, and group II consisted of 32 patients who had been treated with montelukast for at least 6 months. Forty healthy control subjects were also enrolled in the study. Plasma total oxidant status (TOS) and total antioxidant status (TAS) were measured to assess oxidative stress. DNA damage was assessed by means of alkaline comet assay. Results: The patients in both group I and group II had statistically significant higher plasma TOS (13.1 ± 4 and 11.1 ± 4.1 μmol H₂O₂ equivalent/liter, respectively) and low TAS levels (1.4 ± 0.5 and 1.5 ± 0.5 mmol Trolox equivalent/liter, respectively) compared with the control group (TOS: 6.3 ± 3.5 μmol H₂O₂ equivalent/liter and TAS: 2.7 ± 0.6 mmol Trolox equivalent/liter; p < 0.05). DNA damage was 18.2 ± 1.0 arbitrary units (a.u.) in group I, 16.7 ± 8.2 a.u. in group II and 13.7 ± 3.4 a.u. in the control group. There were statistically significant differences only between group I and the control group (p < 0.05). Conclusions: According to the findings, montelukast therapy makes only minimal but not statistically significant improvement in all TOS, TAS and DNA damage parameters.

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Section: Discussionmentioning

confidence: 99%

“…Likewise, although there are some reports that show that allergic diseases can cause increased DNA damage, there are only a few reports assessing if treatment alters such damage [22,23,24]. Apart from 1 in vitro study, there is no information on whether montelukast monotherapy can overcome oxidative stress in asthma [25].…”

Section: Introductionmentioning

confidence: 99%

Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

Dilek

Özkaya²,

Koçyiğit³

et al. 2015

Int Arch Allergy Immunol

Self Cite

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“…A study on DNA damage levels using the Comet assay between smokers and non-smokers was previously reported [27]. In addition, previous studies have also evaluated the degree of DNA damage in healthy vs. patients [28,29]. It has also been shown that antioxidant nutritional supplementation with watercress [30] and grape juice [31] or antioxidant pills such as vitamin E [32] were shown to mitigate DNA damage in smokers.…”

Section: Discussionmentioning

confidence: 99%

The effect of carrot juice, β-carotene supplementation on lymphocyte DNA damage, erythrocyte antioxidant enzymes and plasma lipid profiles in Korean smoker

2011

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High consumption of fruits and vegetables has been suggested to provide some protection to smokers who are exposed to an increased risk of numerous cancers and other degenerative diseases. Carrot is the most important source of dietary β-carotene. Therefore, the objective of this study was to investigate whether carrot juice supplementation to smokers can protect against lymphocyte DNA damage and to compare the effect of supplementation of capsules containing purified β-carotene or a placebo (simple lactose). The study was conducted in a randomized and placebo-controlled design. After a depletion period of 14 days, 48 smokers were supplemented with either carrot juice (n = 18), purified β-carotene (n = 16) or placebo (n = 14). Each group was supplemented for 8 weeks with approximately 20.49 mg of β-carotene/day and 1.2 mg of vitamin C/day, as carrot juice (300 ml/day) or purified β-carotene (20.49 mg of β-carotene, 1 capsule/day). Lymphocyte DNA damage was determined using the COMET assay under alkaline conditions and damage was quantified by measuring tail moment (TM), tail length (TL), and% DNA in the tail. Lymphocyte DNA damage was significantly decreased in the carrot juice group in all three measurements. The group that received purified β-carotene also showed a significant decrease in lymphocyte DNA damage in all three measurements. However, no significant changes in DNA damage was observed for the placebo group except TM (P = 0.016). Erythrocyte antioxidant enzyme was not significantly changed after supplementation. Similarly plasma lipid profiles were not different after carrot juice, β-carotene and placebo supplementation. These results suggest that while the placebo group failed to show any protective effect, carrot juice containing beta-carotene or purified β-carotene itself had great antioxidative potential in preventing damage to lymphocyte DNA in smokers.

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“…In asthmatic patients and experimental animal models of asthma, one of the most referenced form of DNA base damage is 8-oxoG and its open-ringed form FapyG (2,6-diamino-4-hydroxy-5-formamidopyrimidine) [33,34,35,36,37,38,39]. Comprehensive studies have also shown that asthmatic patients have elevated levels of 8-oxoG in their sputum, serum, urine and BALF compared to controls [36,40,41].…”

Section: Guanine Oxidation Is a Marker Of Asthmamentioning

confidence: 99%

8-Oxoguanine DNA glycosylase-1-driven DNA base excision repair

Aguilera-Aguirre

Sur

et al. 2015

Current Opinion in Allergy &Amp; Clinical Immunology

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Purpose of review To provide both an overview and evidence of the potential etiology of oxidative DNA base damage and repair-signaling in chronic inflammation and histological changes associated with asthma. Recent findings Asthma is initiated/maintained by immunological, genetic/epigenetic and environmental factors. It is a world-wide health problem, as current therapies suppress symptoms rather than prevent/reverse the disease, largely due to gaps in understanding its molecular mechanisms. Inflammation, oxidative stress and DNA damage are inseparable phenomena, but their molecular roles in asthma pathogenesis are unclear. It was found that among oxidatively modified DNA bases, 8-oxoguanine (8-oxoG) is one of the most abundant, and its levels in DNA and body fluids are considered a biomarker of ongoing asthmatic processes. Free 8-oxoG forms a complex with 8-oxoguanine DNA glycosylase-1 (OGG1) and activates RAS-family GTPases that induce gene expression to mobilize innate and adaptive immune systems, along with genes regulating airway hyperplasia, hyper-responsiveness and lung remodeling in atopic and non-atopic asthma. Summary DNA’s integrity must be maintained to prevent mutation, so its continuous repair and downstream signaling “fuels” chronic inflammatory processes in asthma, and forms the basic mechanism whose elucidation will allow the development of new drug targets for the prevention/reversal of lung diseases.

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DNA damage in children with asthma bronchiale and its association with oxidative and antioxidative measurements

Cited by 34 publications

References 27 publications

Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

Effect of Montelukast Monotherapy on Oxidative Stress Parameters and DNA Damage in Children with Asthma

The effect of carrot juice, β-carotene supplementation on lymphocyte DNA damage, erythrocyte antioxidant enzymes and plasma lipid profiles in Korean smoker

8-Oxoguanine DNA glycosylase-1-driven DNA base excision repair

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