DNA damage, DNA susceptibility to oxidation and glutathione redox status in patients with Alzheimer's disease treated with and without memantine

Akkaya, Çağlayan; Yavuzer, Serap; Yavuzer, Hakan; Erkol, Gökhan; Bozluolçay, Melda; Dinçer, Yıldız

doi:10.1016/j.jns.2017.04.051

Cited by 16 publications

(8 citation statements)

References 30 publications

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“…When the results were evaluated, it was seen that the patients in the study group were statistically higher than the control group (Table 1). These findings are consistent with previous studies 45,46,55,56 .…”

Section: Discussionsupporting

confidence: 94%

“…In some studies, the level of GSH in individuals with Alzheimer's is lower than in healthy people. Antioxidant use increases in Alzheimer's patients due to the effects of free radicals, resulting in decreased levels of antioxidants [45][46][47] . However, in our study, an increase in GSH values was observed (Table Also, it is a popular test which is applied in epidemiological studies about elderly people living in the community or in an institution 54 .…”

Section: Discussionmentioning

confidence: 99%

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Investigation of the Levels of Blood MDA, GSH and Nitric Oxide Levels in Patients with Probable Alzheimer’s Disease

Güven¹,

Dalginli²,

Çulhaoğlu³

et al. 2020

Kafkas J Med Sci

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Aim:The main focus of this study is to determine blood malondialdehyde (MDA), Glutathione (GSH) and nitric oxide (NO) levels in patients with possible Alzheimer's disease (AD). Material andMethod: The study included 15 healthy persons and 15 Alzheimer's patients aged between 65 and 79 living in Kars. Before taking blood samples from patients who were diagnosed with Alzheimer at Kafkas University Faculty of Medicine Neurology Service, they were applied Standardized Mini Mental State Examination (SMMSE) and Clinical Dementia Rating. In the next step, erythrocyte GSH and serum MDA, NO levels were determined. Results: The difference between healthy persons and Alzheimer's disease group erythrocyte GSH (p<0.05) and serum MDA (p<0.01), NO (p<0.001) levels were statistically significant. MMSE scores were significantly lower than the control group (p<0.001). Conclusion: It was observed that AD caused lipid peroxidation and as a conclusion significantly increased the MDA, GSH and NO levels in the blood of Alzheimer patients. This study supports the theory that the brain is affected by increased oxidative stress in AD based on the data obtained.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionmentioning

confidence: 99%

Investigation of the Levels of Blood MDA, GSH and Nitric Oxide Levels in Patients with Probable Alzheimer’s Disease

Güven¹,

Dalginli²,

Çulhaoğlu³

et al. 2020

Kafkas J Med Sci

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“…It seems that reduction of GSH in AD may be associated with increased oxidative stress and may promote accumulation of DNA oxidation adducts, such as 8-oxo2dG [ 90 ]. The elevated level of 8-oxo2dG was observed in nuclear DNA from frontal, temporal, and parietal lobes and cerebellum of AD patients [ 91 ], as well as in peripheral tissues [ 34 , 37 , 40 , 41 ].…”

Section: Discussionmentioning

confidence: 99%

Biothiols and oxidative stress markers and polymorphisms ofTOMM40andAPOC1genes in Alzheimer’s disease patients

et al. 2018

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Alzheimer’s disease (AD) is a progressive disease, with frequently observed improper biothiols turnover, homocysteine (Hcy) and glutathione (GSH). GSH protects cells from oxidative stress and may be determined by 8-oxo-2’-deoxyguanosine (8-oxo2dG) level and its repair enzyme 8-oxoguanine DNA glycosylase (OGG1). The presence of unfavorable alleles, e.g., in APOE cluster, TOMM40 or APOC1 is known to facilitate the dementia onset under oxidative stress.The aim of the study was to analyze rs1052452, rs2075650 TOMM40 polymorphisms, rs4420638 APOC1, and their correlation with Hcy, GSH, 8-oxo2dG, OGG1 levels in plasma of AD patients and controls.We recruited 230 individuals: 88 AD, 80 controls without (UC), 62 controls with (RC) positive family history of AD. The TOMM40 genotype was determined by HRM and capillary electrophoresis, while APOC1 by HRM. The concentrations of OGG1, 8-oxo2dG were determined by ELISA, whereas Hcy, GSH by HPLC/EC.We showed that over 60% of AD patients had increased Hcy levels (p<0.01 vs. UC, p<0.001 vs. RC), while GSH (p<0.01 vs. UC), 8-oxo2dG (p<0.01 vs. UC, p<0.001 vs. RC) were reduced. Minor variants: rs10524523-L, rs4420638-G, rs2075650-G were significantly overrepresented in AD. For rs4420638-G, rs2075650-G variants, the association remained significant in APOE E4 non-carriers. The misbalance of analyzed biothiols, and 8-oxo2dG, OGG1 were more pronounced in carriers of major variants: rs10524523-S/VL, rs4420638-A, rs2075650-A.We showed, for the first time, that APOC1 and TOMM40 rs2075650 polymorphisms may be independent risk factors of developing AD, whose major variants are accompanied by disruption of biothiols metabolism and inefficient removal of DNA oxidation.

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“…Impaired glutathione redox regulation, increased oxidative stress, and mitochondrial dysfunction are common features of several neurological disorders including autism spectrum disorder or ASD, [1][2][3] psychiatric disorders including schizophrenia [4][5][6] bipolar disorder, 7,8 Alzheimer's disease, 9,10 and Parkinson's disease. [11][12][13] Since glutathione is the major intracellular anti-oxidant in the brain, disruption of glutathione homeostasis by a decrease in the active reduced form of glutathione (GSH) together with an increase in the inactive oxidized disulfide (GSSG) may promote cell damage and neurotoxicity with potential to enhance susceptibility to neurologic disorders.…”

Section: Introductionmentioning

confidence: 99%

Complex epigenetic patterns in cerebellum generated after developmental exposure to trichloroethylene and/or high fat diet in autoimmune-prone mice

Blossom

Melnyk²,

Simmen

2020

Environ. Sci.: Processes Impacts

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DNA damage, DNA susceptibility to oxidation and glutathione redox status in patients with Alzheimer's disease treated with and without memantine

Cited by 16 publications

References 30 publications

Investigation of the Levels of Blood MDA, GSH and Nitric Oxide Levels in Patients with Probable Alzheimer’s Disease

Investigation of the Levels of Blood MDA, GSH and Nitric Oxide Levels in Patients with Probable Alzheimer’s Disease

Biothiols and oxidative stress markers and polymorphisms ofTOMM40andAPOC1genes in Alzheimer’s disease patients

Complex epigenetic patterns in cerebellum generated after developmental exposure to trichloroethylene and/or high fat diet in autoimmune-prone mice

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