DNA damage and repair in type 2 diabetes mellitus

Błasiak, Janusz; Arabski, Michał; Krupa, Renata; Woźniak, Katarzyna; Zadrożny, Marek; Kasznicki, Jacek; Zurawska, Monika; Drzewoski, Józef

doi:10.1016/j.mrfmmm.2004.05.011

Cited by 215 publications

(142 citation statements)

References 42 publications

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“…The slides were examined at 200 Â magnification fluorescence microscope (Nikon, Tokyo, Japan) to a COHU 4910 video camera (Cohu Inc., San Diego, CA) equipped with a UV filter block consist an excitation filter (359 nm) and barrier filter (461 nm) and connected to a personal computer-based image analysis system, Lucia-Comet v.4.51 (Laboratory Imaging, Praha, Czech Republic). Fifty images were randomly selected from each sample and the comet tail DNA was measured (Blasiak et al 2004). Endogenous DNA damage measured as the mean comet tail DNA of peripheral blood lymphocytes of two mice groups (5 mice each).…”

Section: Comet Assaymentioning

confidence: 99%

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Myricitrin and bioactive extract ofAlbizia amaraleaves: DNA protection and modulation of fertility and antioxidant-related genes expression

Kassem

Ibrahim

Hussein

et al. 2016

Pharmaceutical Biology

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Context: Albizia species are reported to exhibit many biological activities including antiovulatory properties in female rats and antispermatogenic and antiandrogenic activities in male rats. Objective: The present study investigates the flavonoids of Albizia amara (Roxb.) B. Boivin (Fabaceae) leaves and evaluates their activity on gene expression of fertility and antioxidant glutathione-S-transferaserelated genes of treated female mice in addition to their effect on DNA damage. Materials and methods: Plant materials were extracted by using 70% methanol for 48 h, the extract was chromatographed on a polyamide 6S column, each isolated compound was purified by using Sephadex LH-20 column; its structure was elucidated by chemical and spectral methods. Both the leaves extract and myricitrin (200, 30 mg/kg bw/d, respectively) were assayed for their effect on DNA damage in female mice after four weeks treatment using Comet assay. Their modulatory activity on gene expression of fertility (aromatase CYP19 and luteinizing hormone LH) and antioxidant glutathione-S-transferase (GST)-related genes of treated female mice were investigated by real-time PCR (qPCR). Results: Quercetin-3-O-gentiobioside, myricitrin, quercetin-3-O-a-rhamnopyranoside, myricetin, quercetin and kaempferol were isolated and identified from the studied taxa. Myricitrin and the extract induced low rate of DNA damage (4.8% and 5%, respectively), compared with the untreated control (4.2%) and significantly down-regulated the expression of CYP19 and LH genes and up-regulated GST gene. Discussion and conclusion: Our results highlight the potential effect of the leaves extract of Albizia amara and myricitrin as fertility-regulating phytoconstituents with ability to protect DNA from damage and cells from oxidative stress. ARTICLE HISTORY

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Section: Comet Assaymentioning

confidence: 99%

“…Endogenous DNA damage measured as the mean comet tail DNA of peripheral blood lymphocytes of two mice groups (5 mice each). The number of cells scored for each animal was 100 (Table 1) (Blasiak et al 2004). …”

Section: Comet Assaymentioning

confidence: 99%

Myricitrin and bioactive extract ofAlbizia amaraleaves: DNA protection and modulation of fertility and antioxidant-related genes expression

Kassem

Ibrahim

Hussein

et al. 2016

Pharmaceutical Biology

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“…Preliminary results show elevated levels of DNA damage in these patients (4.08±3.77) compared to controls (1.1±0.9). The high frequency of MN observed in patients with T2DM may be due to an elevated level of OS that leads to a decrease in glutathione synthesis, because the accumulated products of OS, especially ROS, can cause DNA damage [28].This is consistent with a study done by Martínez-Pérez L. M et al [16]. The levels of buccal MN in diabetic individuals are approximately 2-fold higher than in nondiabetic participants, as revealed by Elisabeth mullner et al [18].…”

Section: Discussionmentioning

confidence: 99%

Micronuclei in exfoliated buccal cells: a biomarker for dna damage leading to progression of late diabetic complications in type 2 diabetes mellitus patients

Biswas¹,

Banerjee²,

Bhattacharya³

et al. 2017

Int j curr adv res

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Elevated levels of DNA damage, decreased efficacy of DNA repair and high susceptibility to mutagens have been associated with type 2 diabetes mellitus. Micronuclei (MN) are a sensitive indicator of genetic damage and a marker of DNA damage. MN is also a morphological marker of chromosomal instability. So the presence of MN in cells reflects structural chromosomal aberrations arising during mitosis. Our aim was to evaluate the frequencies of micronuclei, as a biomarker for DNA damage, in Giemsa stained buccal cells of T2DM patients and healthy controls. A case-control study of seventy six patients with T2DM and twenty five control group participants were recruited from different areas of West Bengal for determination of frequency of MN. All procedures were done with the informed consent of participants. In the present study, frequency of MN found in T2DM patients was 4.08±3.77, which was much higher than the control group participants (1.1±0.9). Frequency of MN increased due to the elevated level of DNA damage in these patients than the controls. Hence, in the present study, increased DNA damage may be due to increased oxidative stress leading to genomic instability, which in turn may lead to the progression of T2DM and its complications and contribute to an increased risk for cancer. This depicts that MN may be a useful constituent in a panel of biomarkers for the risk of late diabetic complications and can be a predisposing factor for development of cancer. INTRODUCTIONType 2 diabetes mellitus (T2DM), the most frequent subtype of diabetes, comprises a complex heterogeneous group of metabolic diseases characterized by high levels of blood glucose (hyperglycaemia) and impaired insulin action and/or insulin secretion.The number of people with T2DM is increasing in every country with 80% of population with DM living in low-and middle-income countries [1]. It is estimated that T2DM affects about 150 million people worldwide and it is expected to rise to about 439 million people by the year 2030 [2,3].India ranked first in the world for the prevalence of the disease, followed by China and USA [4].There are currently approximately 40.9 million patients suffering from diabetes mellitus in India and it is expected to rise to about 69.9 million by the year 2025 [5]. Anticipating an epidemic like increase in the number of diabetic patients, India is being referred to as the 'diabetic capital of the world [6]. About 31.0% populations of West Bengal are suffering from T2DM [7]. The incidence of T2DM varies substantially from one geographical region to the other as a result of environmental and lifestyle risk factors [2]. One of the factors associated with diabetic complications is the process of non-enzymatic glycation of proteins, lipids and nucleic acids, with subsequent formation of advanced glycation end products (AGEs) [8]. One of the main consequences of this adverse action is the formation of oxidative stress (OS). Formation of glycation end products leads to OS and subsequent DNA damage via the oxidation of DNA base...

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“…Another study conducted in Egyptian population also did not find any correlation between duration of diabetes and DNA damage. The discrepancy between different studies is due to the difference in glycemic control, duration of diabetes, or the type of cells used in the comet assay [44].…”

Section: Association Between Tas Dna Damage and Type 2 Diabetes Melmentioning

confidence: 99%

Assessment of Dna Strand Breaks and Total Antioxidant Status in Type 2 Diabetic Patients With and Without Complications- A Case-Control Study

Nithya

Isabel²,

Angeline³

et al. 2017

Asian J Pharm Clin Res

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Objective: To evaluate the total antioxidant status (TAS) and the extent of DNA strand breaks (damage) as a measure of oxidative stress biomarkers in patients with Type 2 diabetes (with and without complications) and controls.Methods: Blood samples were collected from 200 patients with Type 2 diabetes mellitus (n=100, with complications and n=100, without complications) and 100 healthy individuals. Oxidative DNA damage was evaluated using alkaline single cell gel electrophoresis (comet assay). TAS was assessed by ferric reducing ability of plasma assay.Results: TAS was found to be significantly lower in patients with Type 2 diabetes (with and without complications) compared to controls (p<0.001). Similarly, patients with complications of Type 2 diabetes mellitus had significantly lower TAS when compared to patients with diabetes (p=0.007). DNA damage analysis showed that the extent of damage was high in patients with diabetes mellitus (with and without complications) compared to controls (p<0.001). Fasting glucose and glycosylated hemoglobin level (HbA 1C ) were found to be significantly higher in patients with diabetes than controls (p<0.05). Correlation analysis showed that there is no association between age, duration, sugar level, HbA 1C , TAS, and DNA damage in patients with Type 2 diabetes mellitus. Conclusion:Alterations in TAS and the extent of DNA damage were observed in patients with complications of diabetes mellitus indicate that oxidative stress is more in patients with complications when compared to patients without complications and healthy individuals. Therefore, further DNA damage and onset of complications in Type 2 diabetes mellitus could be prevented by counteracting the oxidative stress by therapeutic interventions using appropriate antioxidants.

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DNA damage and repair in type 2 diabetes mellitus

Cited by 215 publications

References 42 publications

Myricitrin and bioactive extract ofAlbizia amaraleaves: DNA protection and modulation of fertility and antioxidant-related genes expression

Myricitrin and bioactive extract ofAlbizia amaraleaves: DNA protection and modulation of fertility and antioxidant-related genes expression

Micronuclei in exfoliated buccal cells: a biomarker for dna damage leading to progression of late diabetic complications in type 2 diabetes mellitus patients

Assessment of Dna Strand Breaks and Total Antioxidant Status in Type 2 Diabetic Patients With and Without Complications- A Case-Control Study

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