DNA damage and cellular death in oral mucosa cells of children who have undergone panoramic dental radiography

Abstract

Taken together, these results indicate that panoramic dental radiography might not induce chromosomal damage, but may be cytotoxic. Overall, the results reinforce the importance of evaluating the health side effects of radiography and contribute to the micronucleus database, which will improve our understanding and practice of this methodology in children.

Cytogenetic biomonitoring in children submitting to a complete set of radiographs for orthodontic planning

“…However, despite higher radiation doses in our investigation, research with similar methodology with dental radiographs showed similar results compared to our study (ie, no mutagenic characteristic was evidenced by the MN test). 6,11,13,14,[17][18][19][20][21] Differences in radiation dose, frequency of exposition, type of cells evaluated, and site of collected cells may influence the results of the MN test. Some authors investigated patients undergoing radiotherapy five to six times per week over the course of 5-7-weeks 16,29 and others observed the effects of frequent occupational exposition to low doses of X-ray in radiation workers, 2,30 whereas some pointed out the results of only a single dental radiograph exposition.…”

“…Some authors investigated patients undergoing radiotherapy five to six times per week over the course of 5-7-weeks 16,29 and others observed the effects of frequent occupational exposition to low doses of X-ray in radiation workers, 2,30 whereas some pointed out the results of only a single dental radiograph exposition. 6,13,[17][18][19][20][21] The literature shows that MN-MNC 31 and cellular death 17 increase with radiation dose. With regard to the different cells employed in the MN assay, radiotherapy is shown to be a potent clastogenic agent in circulating lymphocytes and BEC of head-and-neck cancer patients, 16 but lymphocytes are more sensitive in detecting chromosome aberrations caused by anticancer drugs than are BEC.…”

“…With regard to the different cells employed in the MN assay, radiotherapy is shown to be a potent clastogenic agent in circulating lymphocytes and BEC of head-and-neck cancer patients, 16 but lymphocytes are more sensitive in detecting chromosome aberrations caused by anticancer drugs than are BEC. 25,28 Additionally, in the cytogenetic studies of dental radiograph effects, different sites were elected from which to collect buccal cells: buccal cheek mucosa, 11,13,14,21 the lateral border of the tongue, 17,21 and keratinized mucosa of the upper dental arch. 12 One of these studies 21 showed that the lateral border of the tongue is more sensitive to cytotoxic insult than is the cheek buccal mucosa.…”

“…9 Micronuclei arise from acentric fragments or whole chromosomes that are not included in the main nuclei of the daughter cells and can be induced by agents that cause chromosome breakage or affect the spindle apparatus 10 and can be used as a biomarker in buccal cells to evaluate the mutagenic effect of many agents, including ionizing radiation. 6,[11][12][13][14][15][16][17][18][19][20][21] Previous studies conducted by our research group have demonstrated that the panoramic X-ray is able to induce damage in the oral mucosa cells of children. 13 In this regard, it would be interesting to know if, and to what extent, children submitted to orthodontic radiographic protocol are a more sensitive group as a result of the use of X-ray in oral mucosa cells, particularly because there are no previous reports.…”

“…6,[11][12][13][14][15][16][17][18][19][20][21] Previous studies conducted by our research group have demonstrated that the panoramic X-ray is able to induce damage in the oral mucosa cells of children. 13 In this regard, it would be interesting to know if, and to what extent, children submitted to orthodontic radiographic protocol are a more sensitive group as a result of the use of X-ray in oral mucosa cells, particularly because there are no previous reports. Moreover, no previous biomonitoring study has evaluated the effects of full-mouth X-rays (FMX).…”