DNA damage and aberrant crypt foci as putative biomarkers to evaluate the chemopreventive effect of annatto (Bixa orellana L.) in rat colon carcinogenesis

Agner, Aniele R.; Bazo, Ana Paula; Ribeiro, Lúcia Regina; Salvadori, Daisy Maria Fávero

doi:10.1016/j.mrgentox.2005.01.009

Cited by 54 publications

(26 citation statements)

References 28 publications

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“…This result pointed to the need for a sequential test with medium or long-term studies that would evaluate tumor biomarkers or even installed neoplasia (Maurin et al, 2007). Given the above, we have chosen a medium-term test, the aberrant crypt focus assay (Bazo et al, 2002;Agner et al, 2005).…”

Section: Discussionmentioning

confidence: 99%

Effects of Moquiniastrum polymorphum ssp floccosum ethnolic extract on colorectal carcinogenesis induced by 1,2-dimethylhydrazine

Limeiras¹,

Oliveira²,

Pessatto³

et al. 2017

Genet. Mol. Res.

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ABSTRACT. The objective of this study was to evaluate the effect of Moquiniastrum polymorphum ssp floccosum ethanolic extract (MPEE) on 1,2 dimethylhydrazine (DMH)-induced colorectal carcinogenesis in mice. Forty-two male Swiss mice (Mus musculus) were subdivided into six groups (N = 7/group): negative control, DMH, MPEE, pre-treatment, simultaneous, and post-treatment. Results showed that MPEE has antigenotoxic potential on the tested protocols pre-and silmultaneous treatment, and the percent damage reductions (%DRs) were 81.88 and 93.12%, respectively. The micronucleus test demonstrated that MPEE has great antimutagenic activity, with %DRs higher than 77.09 in the associated groups. The aberrant crypt focus assay demonstrated anticarcinogenic potential of MPEE as the associated groups showed %DRs that ranged from 62.13 to 95.14%. The study shows that MPEE is nontoxic and has chemopreventive and anticarcinogenic activity, thus it may prove to be a promising medicinal plant in view of its demonstrated properties.

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Section: Discussionmentioning

confidence: 99%

Effects of Moquiniastrum polymorphum ssp floccosum ethnolic extract on colorectal carcinogenesis induced by 1,2-dimethylhydrazine

Limeiras¹,

Oliveira²,

Pessatto³

et al. 2017

Genet. Mol. Res.

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“…From this production 70% are used in cooking, 20% as natural dye or as food coloring, and 10% to exportation. Agner et al (2005) studied effect of annatto on preneoplasic lesions induced by DMH and on DNA damage on rat's colons. Under experimental conditions, annato did not develop any adverse effect and results suggested possible chemopreventive effects by its cellular proliferation modulation, but not in the carcinogenesis initial stage (Agner et al, 2005).…”

Section: Introductionmentioning

confidence: 99%

“…Agner et al (2005) studied effect of annatto on preneoplasic lesions induced by DMH and on DNA damage on rat's colons. Under experimental conditions, annato did not develop any adverse effect and results suggested possible chemopreventive effects by its cellular proliferation modulation, but not in the carcinogenesis initial stage (Agner et al, 2005). In studies with rabbits, bixin and norbixin, carothenoids found in annatto, yielded promising results for a possible future use to either prevent or treat cardiac illness (Lima et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Antimycobacterial activity evaluation and MIC determination of liophilizated hydroalcoholic extracts of Bixa orellana L., Bixaceae

Silva¹,

Almeida²,

Chavasco³

et al. 2010

Rev. bras. farmacogn.

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RESUMO: "Avaliação da atividade antimicobacteriana e a determinação da CIM de extratos hidroalcoólicos liofilizados de Bixa orellana L., Bixaceae." Bixa orellana é usada na medicina popular em várias doenças. Extratos do fruto, raiz e folhas apresentaram atividade antimicrobiana, enquanto extratos do caule mostraram resultados negativos. Este estudo teve por objetivo verificar a atividade antimicobacteriana de extratos hidroalcoólicos liofilizados de B. orellana frente Mycobacterium tuberculosis e determinar a Concentração Inibitória Mínima para cinco bactérias. A atividade antimicobacteriana foi determinada por difusão e a CIM por difusão e análise colorimétrica. As CIM foram 0,3, 0,5 e 0,2 mg/mL para os extratos de folhas, raiz e caule frente M. tuberculosis. Os extratos de caule demonstraram CIM de 1,12 mg/mL (B. cereus), 1,53 mg/mL (S. aureus e S. typhimurium), 4,50 mg/mL (P. aeruginosa) e 8,01 mg/mL (P. mirabillis); extratos de folhas mostraram 0,66 mg/mL (P. aeruginosa), 0,94 mg/mL (P. mirabillis), 1,88 mg/mL (S. aureus), 3,95 mg/mL (B. cereus) e 8,37 mg/mL (S. typhimurium); extratos de raiz demonstraram 0,25 mg/mL (P. aeruginosa), 0,31 mg/mL (S. aureus), 0,62 mg/mL (S. typhimurium) e 3,00 mg/ mL (B. cereus e P. mirabillis). Extratos de folhas e caule mostraram atividade antimicobacteriana. As CIM por colorimetria foram menores que na difusão em agar. Os extratos revelaram atividade bacteriostática sobre as cinco espécies bacterianas. Unitermos: Mycobacterium tuberculosis, Bixa orellana, Bixaceae.ABSTRACT: Bixa orellana is used in popular medicine against several diseases. Extracts of its fruit, root and leaf presented antimicrobial activity, while seed extract showed negative results. This study aimed at verifying the antimycobacterial activity of B. orellana lyophilized hydroalcoholic extracts over Mycobacterium tuberculosis, and determine the Minimum Inhibitory Concentration against five bacteria. Antimycobacterial activity was determined by diffusion technique, while MIC was assessed by diffusion and colorimetric analysis. MICs were 0.3, 0.5 and 0.2 mg/mL respectively, for leaf, root and stem extracts, against M. tuberculosis. Stem's extract showed 1.2 mg/mL for B. cereus, 1.53 mg/mL for S. aureus and S. typhimurium, 4.50 mg/mL for P. aeruginosa and 8.01 mg/mL for P. mirabillis. Leaf extracts showed 0.66 mg/mL for P. aeruginosa, 0.94 mg/ mL for P. mirabillis, 1.88 mg/mL for S. aureus, 3.95 mg/mL for B. cereus and 8.37 mg/mL for S. typhimurium. Root's extracts showed 0.25 mg/mL for P. aeruginosa, 0.31 mg/mL for S. aureus, 0.62 mg/mL for S. typhimurium and 3.00 mg/mL for B. cereus and P. mirabillis. Leaf and stem extracts showed antimycobacterial activity. MICs were lower in colorimetric analysis than in agar diffusion. Extracts revealed bacteriostatic activity against the five bacterial.

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“…Other chemopreventive agents with antioxidant properties have been determined to inhibit DMH-induced colon carcinogenesis and DNA damage in an animal model. 38,39) It has been reported that bacteria, or their extracts, exerted antioxidative effects in vitro, [40][41][42] and also evidenced a protective effect against hemolysis in vitamin E-deficient rats. 43) Ito et al reported that 2 weeks of a diet containing 0.4% Streptococcus thermophilus YIT 2001 (2ϫ10 8 cfu/d) induced a significant reduction in lipid peroxides (thiobarbituric acid reactive substances) in the colonic mucosa of iron-overloaded mice.…”

Section: Discussionmentioning

confidence: 99%

A Probiotic Strain of Bacillus polyfermenticus Reduces DMH Induced Precancerous Lesions in F344 Male Rat

Park

Jeon

Park

et al. 2007

Biological & Pharmaceutical Bulletin

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DNA damage and aberrant crypt foci as putative biomarkers to evaluate the chemopreventive effect of annatto (Bixa orellana L.) in rat colon carcinogenesis

Cited by 54 publications

References 28 publications

Effects of Moquiniastrum polymorphum ssp floccosum ethnolic extract on colorectal carcinogenesis induced by 1,2-dimethylhydrazine

Effects of Moquiniastrum polymorphum ssp floccosum ethnolic extract on colorectal carcinogenesis induced by 1,2-dimethylhydrazine

Antimycobacterial activity evaluation and MIC determination of liophilizated hydroalcoholic extracts of Bixa orellana L., Bixaceae

A Probiotic Strain of Bacillus polyfermenticus Reduces DMH Induced Precancerous Lesions in F344 Male Rat

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