DNA content of Ovis musimon spermatozoa

Ferrari, María Rosa; Spirito, S. E.; Giuliano, S.; Cisale, H.

doi:10.1111/j.1439-0272.2011.01160.x

Cited by 1 publication

(3 citation statements)

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“…The haploid DNA content (C‐DNA) per sperm nucleus expressed in picograms was calculated using G. domesticus erythrocytes as a standard (C‐value = 1.25 pg, Gregory, ) and according to Spirito, Campi, Boquet, Fernández, and Ferrari () and Ferrari et al. (). The scanning method allowed obtaining densitometric parameters related to the DNA distribution and condensation in each sperm nucleus: point absorbance (measure of the absorbance of each of the points), mean absorbance (mean obtained from the absorbance of all the points), maximal absorbance (absorbance of the point with the largest absorbance) and maximal absorbance/mean absorbance (relation between maximal and mean absorbance).…”

Section: Methodsmentioning

confidence: 99%

“…DNA content was measured in a pool of 210 morphologically normal sperm nuclei obtained from seven llama males. The amount of DNA–Feulgen complex, expressed in arbitrary units as mean absorbance per area, was obtained using the scanning method in a Cytoscan Zeiss Universal microscope (UMSP 30, Oberkochen, Germany) at a wavelength of 560 nm according to Ferrari, Spirito, Giuliano, and Cisale ().…”

Section: Methodsmentioning

confidence: 99%

“…The haploid DNA content (C-DNA) per sperm nucleus expressed in picograms was calculated using G. domesticus erythrocytes as a standard (C-value = 1.25 pg, Gregory, 2016) and according to Spirito, Campi, Boquet, Fernández, andFerrari (2011) andFerrari et al (2012).…”

Section: Determination Of the Haploid Dna Content (C-value) And Parmentioning

confidence: 99%

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Morphology, morphometry and chromatin distribution in llama sperm nuclei

Giuliano

Cisale

Ferrari

2018

Reprod Domestic Animals

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Contents The objectives of this study were as follows: (i) to describe and evaluate the frequencies of different morphologies of llama sperm nuclei, (ii) to determine morphometric values of nuclear parameters, (iii) to describe and estimate the frequencies of different classes of chromatin distribution and (iv) to measure haploid DNA content and analyse its nuclear distribution. The study was performed using ejaculates collected from seven males, and sperm nuclei were stained with the Feulgen reaction. Normal morphology ranged from 78.36% to 93.92%, and abnormalities included short, small, large, pyriform, narrow, micro and round nuclei. Important differences in nuclei considered normal were found between some males. The following average values were obtained for each sperm nuclear morphometric parameter analysed: area 11.64 μm2, perimeter 13.16 μm, length 5.12 μm, width 2.81 μm, ellipticity 1.85 and form 0.83. Differences between males were significant for all the parameters (p < .01). Light microscope observations and cytophotometric determinations allowed discriminating between three classes of chromatin distribution: homogeneous, diffuse and showing a clear band. Significant differences between males were found for the frequencies of the three classes (p < .01). Cluster analysis methods were used to estimate the resemblance between males according to the characteristics of their sperm nuclei. A great intermale variability was found for morphological, morphometric and chromatin distribution data. These parameters would have low dependence between them.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%