DNA cleavage mediated by copper superoxide dismutase via two pathways

Han, Yingchun; Shen, Tao; Jian, Wei; Xia, Q.; Liu, Changlin

doi:10.1016/j.jinorgbio.2006.09.014

Cited by 19 publications

(11 citation statements)

References 43 publications

(61 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Similarly, hydrogen peroxide with the addition of SOD also improved the cleavage yield of PLDz (Supplementary Figure 10 ). This result is consistent with the earlier result about DNA cleavage mediated by H 2 O 2 /SOD by Han et al 33 . …”

Section: Resultssupporting

confidence: 94%

New cofactors and inhibitors for a DNA-cleaving DNAzyme: superoxide anion and hydrogen peroxide mediated an oxidative cleavage process

Sun

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

Herein, we investigated the effects of new cofactors and inhibitors on an oxidative cleavage of DNA catalysis, known as a pistol-like DNAzyme (PLDz), to discuss its catalytic mechanism. PLDz performed its catalytic activity in the presence of ascorbic acid (AA), in which Cu2+ promoted, whereas Fe2+ significantly inhibited the catalytic function. Since Fe2+/AA-generated hydroxyl radicals are efficient on DNA damage, implying that oxidative cleavage of PLDz had no relation with hydroxyl radical. Subsequently, we used Fe2+/H2O2 and Cu2+/H2O2 to identify the role of hydroxyl radicals in PLDz catalysis. Data showed that PLDz lost its activity with Fe2+/H2O2, but exhibited significant cleavage with Cu2+/H2O2. Because Fe2+/H2O2 and Cu2+/H2O2 are popular reagents to generate hydroxyl radicals and the latter also produces superoxide anions, we excluded the possibility that hydroxyl radical participated in oxidative cleavage and confirmed that superoxide anion was involved in PLDz catalysis. Moreover, pyrogallol, riboflavin and hypoxanthine/xanthine oxidase with superoxide anion and hydrogen peroxide generation also induced self-cleavage of PLDz, where catalase inhibited but superoxide dismutase promoted the catalysis, suggesting that hydrogen peroxide played an essential role in PLDz catalysis. Therefore, we proposed a catalytic mechanism of PLDz in which superoxide anion and hydrogen peroxide mediated an oxidative cleavage process.

show abstract

Section: Resultssupporting

confidence: 94%

New cofactors and inhibitors for a DNA-cleaving DNAzyme: superoxide anion and hydrogen peroxide mediated an oxidative cleavage process

Sun

Wang

et al. 2017

Sci Rep

View full text Add to dashboard Cite

show abstract

“…However, there is one important hindrance, as both SOD and SOD-like Cu-complexes can cleave cellular DNA by reacting with hydrogen peroxide and generating hydroxyl radicals by Fenton-type reaction (Sang and Yang 2005; Han et al 2007; Seng et al 2009; Ghosh et al 2010; Ibrahim et al 2011). This aspect requires thorough dose–response studies, before this group of potential anti-inflammatory drugs can emerge.…”

Section: Biological Activity Of Complexes Of Stable Copper Isotopesmentioning

confidence: 99%

Development of copper based drugs, radiopharmaceuticals and medical materials

et al. 2012

View full text Add to dashboard Cite

Copper is one of the most interesting elements for various biomedical applications. Copper compounds show vast array of biological actions, including anti-inflammatory, anti-proliferative, biocidal and other. It also offers a selection of radioisotopes, suitable for nuclear imaging and radiotherapy. Quick progress in nanotechnology opened new possibilities for design of copper based drugs and medical materials. To date, copper has not found many uses in medicine, but number of ongoing research, as well as preclinical and clinical studies, will most likely lead to many novel applications of copper in the near future.

show abstract

“…SOD1 was chelated with ETDA to give apo-SOD1, which was treated with ZnCl 2 or CuSO 4 (Wako Pure Chemical Industries) to produce Zn-SOD1 or Cu-SOD1, as reported previously. 23 We measured each protein concentration using a protein assay kit (BCA Protein Assay Kit, Pierce, Rockland, IL, USA) and 12.5 mg of each protein was applied to the gel. Proteins containing SOD1, which was detected using an anti-SOD1 antibody (sc-11407, FL-154, Santa Cruz Biotechnology, Texas, USA), from rat liver (LEC rat and F344, Charles River Laboratories Japan, Inc., Kanagawa, Japan) 24 were prepared using gel filtration and a highperformance liquid chromatography (HPLC) column (7.5 Â 600 mm, with a 7.5 Â 75 mm guard column; Tsk gel G3000SW, Tosoh, Tokyo, Japan) by eluting with 10 mM Tris-HCl, 100 mM Na 2 SO 4 (pH 7.0; 25 1C), at a flow rate of 1.0 mL min À1 , with in-line detection.…”

Section: Methodsmentioning

confidence: 99%

Scanning protein analysis of electrofocusing gels using X-ray fluorescence

Matsuyama¹,

Matsunaga²,

Sakamoto³

et al. 2013

Metallomics

View full text Add to dashboard Cite

Recently, "metallomics," in addition to genomics and proteomics, has become a focus as a novel approach to identify sensitive fluctuations in homeostasis that accompany metabolic processes, such as stress responses, differentiation, and proliferation. Cellular elements and associated protein behavior provide important clues for understanding cellular and disease mechanism(s). It is important to develop a system for measuring the native status of the protein. In this study, we developed an original freeze-dried electrofocusing native gel over polyimide film (native-gel film) for scanning protein analysis using synchrotron radiation excited X-ray fluorescence (SPAX). To our knowledge, this is the first report detailing the successful mapping of metal-associated proteins of electrofocusing gels using X-ray fluorescence. SPAX can provide detection sensitivity equivalent to that of LA-ICP-MS. In addition to this increased sensitivity, SPAX has the potential to be combined with other X-ray spectroscopies. Our system is useful for further applications in proteomics investigating cellular element-associated protein behaviors and disease mechanisms.

show abstract

DNA cleavage mediated by copper superoxide dismutase via two pathways

Cited by 19 publications

References 43 publications

New cofactors and inhibitors for a DNA-cleaving DNAzyme: superoxide anion and hydrogen peroxide mediated an oxidative cleavage process

New cofactors and inhibitors for a DNA-cleaving DNAzyme: superoxide anion and hydrogen peroxide mediated an oxidative cleavage process

Development of copper based drugs, radiopharmaceuticals and medical materials

Scanning protein analysis of electrofocusing gels using X-ray fluorescence

Contact Info

Product

Resources

About