DNA Cleavage and Condensation Activities of Mono- and Binuclear Hybrid Complexes and Regulation by Graphene Oxide

Abstract

Hybrid complexes with N,N 1 -bis(2-benzimidazolylmethyl)amine and cyclen moieties are novel enzyme mimics and controlled DNA release materials, which could interact with DNA through three models under different conditions. In this paper, the interactions between plasmid DNA and seven different complexes were investigated, and the methods to change the interaction patterns by graphene oxide (GO) or concentrations were also investigated. The cleavage of pUC19 DNA promoted by target complexes were via hydrolytic or oxidative mechanisms at low concentrations ranging from 3.13ˆ10´7 to 6.25ˆ10´5 mol/L. Dinuclear complexes 2a and 2b can promote the cleavage of plasmid pUC19 DNA to a linear form at pH values below 7.0. Furthermore, binuclear hybrid complexes could condense DNA as nanoparticles above 3.13ˆ10´5 mol/L and partly release DNA by graphene oxide with π-π stacking. Meanwhile, the results also reflected that graphene oxide could prevent DNA from breaking down. Cell viability assays showed dinuclear complexes were safe to normal human hepatic cells at relative high concentrations. The present work might help to develop novel strategies for the design and synthesis of DNA controllable releasing agents, which may be applied to gene delivery and also to exploit the new application for GO.

Photo‐excitable zinc sulfide nanoparticles: A theranostic nanotool for cancer management

“…In agarose gel electrophoresis, DNA plasmid migrates faster in its intact supercoiled circular form (Form I) than the slower relaxed nicked form (Form II) scissored in one strand. Meanwhile, if both strands are cleaved, a linear form (Form III) will be generated that migrates intermediately between Forms I and II (Li et al, 2016; Shahida Parveen et al, 2014). Our nicked and linear plasmids treated with UV‐activated ZnS NPs revealed the capability of photoactivated quantum dots to act as extrinsic nucleases in cutting DNA strands through emitting energetic electrons capable of generating H 2 O 2 .…”