DNA biosensors based on gold nanoparticles-modified graphene oxide for the detection of breast cancer biomarkers for early diagnosis

Saeed, Ayman Ali; Sánchez, Josep Lluís Acero; O’Sullivan, Ciara K.; Abbas, Mohammed Nooredeen

doi:10.1016/j.bioelechem.2017.07.002

Cited by 135 publications

(59 citation statements)

References 62 publications

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“…In recent years, an increasing amount of research has focused on the use of AuNP-GO hybrids in biosensors. These hybrids have been shown to be useful in the development of electrochemical [ 54 – 59 ] and surface-enhanced Raman scattering (SERS) [ 56 , 59 ] platform technology to improve the application in biological assays. However, there are currently no relevant reports on the use of naked eye or colorimetric rapid immunoassay biosensor technology.…”

Section: Introductionmentioning

confidence: 99%

“…For example, electrochemical DNA biosensors based on AuNP-GO hybrids have been used to detect breast cancer biomarkers to allow for an early diagnosis. With this biosensor, the detection limit (LOD) of 0.16 nM was obtained with a sensitivity of 378 nA/nM for the ERBB2 biomarker [ 54 ]. In addition, an AuNP dotted reduction graphene oxide (rGO-AuNP) nanocomposite-based electrochemical aptasensor has been used to selectively detect a concentration of 3,3′4,4′-polychlorinated biphenyls (PCB77) between 1 pg L − 1 and 10 μg L − 1 , with a LOD of 0.1 pg L − 1 [ 55 ].…”

Section: Introductionmentioning

confidence: 99%

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Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

et al. 2018

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In this study, we propose a modified gold nanoparticle-graphene oxide sheet (AuNP-GO) nanocomposite to detect two different interactions between proteins and hybrid nanocomposites for use in biomedical applications. GO sheets have high bioaffinity, which facilitates the attachment of biomolecules to carboxyl groups and has led to its use in the development of sensing mechanisms. When GO sheets are decorated with AuNPs, they introduce localized surface plasmon resonance (LSPR) in the resonance energy transfer of spectral changes. Our results suggest a promising future for AuNP-GO-based label-free immunoassays to detect disease biomarkers and rapidly diagnose infectious diseases. The results showed the detection of antiBSA in 10 ng/ml of hCG non-specific interfering protein with dynamic responses ranging from 1.45 nM to 145 fM, and a LOD of 145 fM. Considering the wide range of potential applications of GO sheets as a host material for a variety of nanoparticles, the approach developed here may be beneficial for the future integration of nanoparticles with GO nanosheets for blood sensing. The excellent anti-interference characteristics allow for the use of the biosensor in clinical analysis and point-of-care testing (POCT) diagnostics of rapid immunoassay products, and it may also be a potential tool for the measurement of biomarkers in human serum.Electronic supplementary materialThe online version of this article (10.1186/s11671-018-2565-7) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

et al. 2018

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“…These results indicate that the proposed DNA sensor based on MWCNTs and TSPP probably exhibits a high sensitivity for all previously ascribed reasons such as the high specific surface of CNTs, the short distance between porphyrin and CNTs that increases electron transfer [ 19 , 23 ], and especially different attractions or repulsions occurring among CNTs, ssDNA/dsDNA and anionic porphyrin, which produce synergetic effects to amplify the impedance difference to be big enough for target DNA detection without any label. As can be seen from Table 3 , compared with some biosensors for the detection of DNA specific to genes from the same family of tyrosine kinase receptor [ 43 , 44 , 45 , 46 , 47 ], such as HER2 (also known as ERBB2 or c-erbB-2) and HER1 (also known as EGFR [ 48 ]), our work presents an excellent sensing platform that can be constructed in a quite simple way with good sensitivity and a wide dynamic range ( Figure 3 ).…”

Section: Resultsmentioning

confidence: 99%

Abnormal Anionic Porphyrin Sensing Effect for HER2 Gene Related DNA Detection via Impedance Difference between MWCNTs and Single-Stranded DNA or Double-Stranded DNA

et al. 2018

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Human epidermal growth factor receptor 2 (HER2) is a key tumor marker for several common and deadly cancers. It is of great importance to develop efficient detection methods for its over-expression. In this work, an electrochemical impedance spectroscopy (EIS) method adjustable by anionic porphyrin for HER2 gene detection has been proposed, based on the impedance difference between multi-walled carbon nanotubes (MWCNTs) and DNA. The interesting finding herein is that with the addition of anionic porphyrin, i.e., meso-tetra(4-sulfophenyl)-porphyrin (TSPP), the impedance value obtained at a glass carbon electrode (GCE) modified with MWCNTs and a single stranded DNA (ssDNA), the probe DNA that might be assembled tightly onto MWCNTs through π-π stacking interaction, gets a slight decrease; however, the impedance value from a GCE modified with MWCNTs and a double stranded DNA (dsDNA), the hybrid of the probe DNA with a target DNA, which might be assembled loosely onto MWCNTs for the screening effect of phosphate backbones in dsDNA, gets an obvious decrease. The reason may be that on the one hand, being rich in negative sulfonate groups, TSPP will try to push DNA far away from CNTs surface due to its strong electrostatic repulsion towards DNA; on the other hand, rich in planar phenyl or pyrrole rings, TSPP will compete with DNA for the surface of CNTs since it can also be assembled onto CNTs through conjugative interactions. In this way, the “loosely assembled” dsDNA will be repelled by this anionic porphyrin and released off CNTs surface much more than the “tightly assembled” ssDNA, leading to a bigger difference in the impedance value between dsDNA and ssDNA. Thus, through the amplification effect of TSPP on the impedance difference, the perfectly matched target DNA could be easily determined by EIS without any label. Under the optimized experimental conditions, this electrochemical sensor shows an excellent linear response to target DNA in a concentration range of 2.0 × 10−11–2.0 × 10−6 M with a limit of detection (LOD) of 6.34 × 10−11 M (S/N = 3). This abnormally sensitive electrochemical sensing performance resulting from anionic porphyrin for DNA sequences specific to HER2 gene will offer considerable promise for tumor diagnosis and treatment.

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“…GR nanocomposites with inorganic nanostructures [ 76 , 77 , 78 , 79 ], conducting polymers [ 80 , 81 , 82 , 83 ] and organic materials [ 84 ] can enhance the analytical response of sensors and biosensors, mainly improving their sensitivity, limit of detection, and reproducibility of electrochemical biosensors for the detection of biomolecules. Functionalized GR/reduced GO (rGO) with a variety of materials, iridium oxide [ 85 ], Au nanostructures [ 86 , 87 , 88 , 89 ], AgNP [ 90 , 91 ], FeNP [ 92 ], PtNPs [ 93 , 94 , 95 , 96 ], Pd–Pt NPs [ 97 , 98 ], TiO 2 NPs [ 99 , 100 ], CuO 2 NPs [ 101 ], ZrO 2 NPs [ 102 ], or CNTs [ 103 ], have been used as electrochemical biosensors for enhancing the electron transfer property between electrode substrates and enzymes.…”

Section: Graphene (Gr) For Electrochemical Biosensorsmentioning

confidence: 99%

Carbon Nanomaterials as Versatile Platforms for Biosensing Applications

et al. 2020

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A biosensor is defined as a measuring system that includes a biological receptor unit with distinctive specificities toward target analytes. Such analytes include a wide range of biological origins such as DNAs of bacteria or viruses, or proteins generated from an immune system of infected or contaminated living organisms. They further include simple molecules such as glucose, ions, and vitamins. One of the major challenges in biosensor development is achieving efficient signal capture of biological recognition-transduction events. Carbon nanomaterials (CNs) are promising candidates to improve the sensitivity of biosensors while attaining low detection limits owing to their capability of immobilizing large quantities of bioreceptor units at a reduced volume, and they can also act as a transduction element. In addition, CNs can be adapted to functionalization and conjugation with organic compounds or metallic nanoparticles; the creation of surface functional groups offers new properties (e.g., physical, chemical, mechanical, electrical, and optical properties) to the nanomaterials. Because of these intriguing features, CNs have been extensively employed in biosensor applications. In particular, carbon nanotubes (CNTs), nanodiamonds, graphene, and fullerenes serve as scaffolds for the immobilization of biomolecules at their surface and are also used as transducers for the conversion of signals associated with the recognition of biological analytes. Herein, we provide a comprehensive review on the synthesis of CNs and their potential application to biosensors. In addition, we discuss the efforts to improve the mechanical and electrical properties of biosensors by combining different CNs.

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DNA biosensors based on gold nanoparticles-modified graphene oxide for the detection of breast cancer biomarkers for early diagnosis

Cited by 135 publications

References 62 publications

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

Enhanced Plasmonic Biosensors of Hybrid Gold Nanoparticle-Graphene Oxide-Based Label-Free Immunoassay

Abnormal Anionic Porphyrin Sensing Effect for HER2 Gene Related DNA Detection via Impedance Difference between MWCNTs and Single-Stranded DNA or Double-Stranded DNA

Carbon Nanomaterials as Versatile Platforms for Biosensing Applications

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