DNA biochip arraying, detection and amplification strategies

Campàs, Mònica; Katakis, Ioanis

doi:10.1016/s0165-9936(04)00104-9

Cited by 108 publications

(72 citation statements)

References 44 publications

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“…The need for the production of low-cost microarrays with high spots density, precision, and repeatability has resulted in extensive research devoted to the design and development of different arraying techniques, the most extended ones being photolithography, pin deposition, and ink-jet printing. 1,2 Laser-induced forward transfer (LIFT) is a versatile noncontact technique that appears to be an interesting alternative to the conventional arraying techniques, since it avoids the use of expensive photolithographic processes and, thanks to the high focusing power of lasers, presents higher integration scales than pin deposition and ink-jet printing. Its method of operation consists in the focusing of a pulsed laser beam on a thin film of the material to be deposited through the film support transparent to the laser radiation.…”

mentioning

confidence: 99%

Preparation of functional DNA microarrays through laser-induced forward transfer

Serra

Colina

Fernández-Pradas

et al. 2004

Applied Physics Letters

166

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A functional DNAmicroarray was prepared through the laser-induced forward transfer (LIFT) technique. In a first experiment, droplets of a buffer solution were spotted onto a substrate at different laser pulse energies. This allowed one to determine that uniform spots with a diameter as small as 40μm could be obtained. In a second experiment, a microarray containing two different human cDNAs and a negative control was spotted through LIFT and submitted to a hybridization assay. The obtained results demonstrated the full functionality of the microarray, which allowed us to prove the viability of LIFT for the production of DNAmicroarrays.Postprint (published version

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mentioning

confidence: 99%

Preparation of functional DNA microarrays through laser-induced forward transfer

Serra

Colina

Fernández-Pradas

et al. 2004

Applied Physics Letters

166

View full text Add to dashboard Cite

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“…Winzor and Chan and Chen highlight the biosensor's contributions in affinity chromatography [93] and characterization of protein-fouled membranes [11]. Brandt and Hoheisel, Campàs and Katakis, Disney and Seeberger, Espina et al, Michaud, Pavlivkova et al, and Walsh and Chang each discuss the applicability of SPR as a detection method in the development of higherthroughput protein, carbohydrate, DNA and small molecule array platforms [6,8,24,27,59,68,90], Lion et al summarizes the importance of microfluidics in Biacore biosensors [49], and Buijs and Natsume, Damle et al, Mattei et al and Stuhler and Meyer describe coupling the biosensor with mass spectrometry to streamline isolation and identification of bound analytes from crude materials [7,18,57,84].…”

Section: Reviews Theory and Methodsmentioning

confidence: 99%

Survey of the year 2004 commercial optical biosensor literature

Rich

Myszka

2005

J of Molecular Recognition

114

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The year 2004 represents a milestone for the biosensor research community: in this year, over 1000 articles were published describing experiments performed using commercially available systems. The 1038 papers we found represent a $ 10% increase over the past year and demonstrate that the implementation of biosensors continues to expand at a healthy pace. We evaluated the data presented in each paper and compiled a 'top 10' list. These 10 articles, which we recommend every biosensor user reads, describe wellperformed kinetic, equilibrium and qualitative/screening studies, provide comparisons between binding parameters obtained from different biosensor users, as well as from biosensor-and solution-based interaction analyses, and summarize the cutting-edge applications of the technology. We also re-iterate some of the experimental pitfalls that lead to sub-optimal data and over-interpreted results. We are hopeful that the biosensor community, by applying the hints we outline, will obtain data on a par with that presented in the 10 spotlighted articles. This will ensure that the scientific community at large can be confident in the data we report from optical biosensors.

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“…Nucleic acid microarrays or protein microarrays have been widely successful in multiplexed detection offering high detection throughput [148,149]. Multiplexing is usually achieved by the use of a range of fluorophores, each capable of detecting a specific target either in a labeled or in a labelfree format [27].…”

Section: Multiplexingmentioning

confidence: 99%

Towards in vitro molecular diagnostics using nanostructures

Kurkina

Balasubramanian

2011

Cell. Mol. Life Sci.

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Nanostructures appear to be promising for a number of applications in molecular diagnostics, mainly due to the increased surface-to-volume ratio they can offer, the very low limit of detection achievable, and the possibility to fabricate point-of-care diagnostic devices. In this paper, we review examples of the use of nanostructures as diagnostic tools that bring in marked improvements over prevalent classical assays. The focus is laid on the various sensing paradigms that possess the potential or have demonstrated the capability to replace or augment current analytical strategies. We start with a brief introduction of the various types of nanostructures and their physical properties that determine the transduction principle. This is followed by a concise collection of various functionalization protocols used to immobilize biomolecules on the nanostructure surface. The sensing paradigms are discussed in two contexts: the nanostructure acting as a label for detection, or the nanostructure acting as a support upon which the molecular recognition events take place. In order to be successful in the field of molecular diagnostics, it is important that the nanoanalytical tools be evaluated in the appropriate biological environment. The final section of the review compiles such examples, where the nanostructure-based diagnostic tools have been tested on realistic samples such as serum, demonstrating their analytical power even in the presence of complex matrix effects. The ability of nanodiagnostic tools to detect ultralow concentrations of one or more analytes coupled with portability and the use of low sample volumes is expected to have a broad impact in the field of molecular diagnostics.

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DNA biochip arraying, detection and amplification strategies

Cited by 108 publications

References 44 publications

Preparation of functional DNA microarrays through laser-induced forward transfer

Preparation of functional DNA microarrays through laser-induced forward transfer

Survey of the year 2004 commercial optical biosensor literature

Towards in vitro molecular diagnostics using nanostructures

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