DNA-binding, DNA cleavage and cytotoxicity studies of two anthraquinone derivatives

Gholivand, Mohammad Bagher; Kashanian, Soheila; Peyman, Hossein

doi:10.1016/j.saa.2011.11.045

Cited by 25 publications

(15 citation statements)

References 60 publications

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“…The current conventional miRNA detection strategies are majorly expensive, time-consuming and need a complex instruments and perhaps a qualified and trained operator. 13,14 Anthraquinones are redox-active molecules which can be utilized for electrochemical labelling of biomolecules and some of their derivatives are proven as remarkable intercalators. Electrochemical biosensing methods are combining the high selectivity of the biochemical recognition with the high sensitivity of the electrochemical detection.…”

Section: Introductionmentioning

confidence: 99%

Application of Oracet Blue in a novel and sensitive electrochemical biosensor for the detection of microRNA

et al. 2015

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MicroRNAs in serum/plasma, have been introduced as a novel, reliable and specific biomarkers for detection, status monitoring and population screening of a disease. Along with the molecular biology techniques, electrochemical biosensors have become the major biosensing methods for miRNA quantification. Here we have tried to develop an electrochemical biosensing system which is simple, easy to prepare, highly sensitive and selective, cost-effective and no need for sample preparation and/or amplification. We have evaluated an anthraquinone compound Oracet Blue (OB), as an intercalative electroactive label for miRNA electrochemical biosensing. The proposed electrochemical biosensor was made of a thiolated single strand capture probe (SH-modified SS-Probe) on an Au electrode (AuE). The role of OB was electrochemical signal transducing upon hybridization of the SS-Probe to the target miRNA. Under optimized conditions, target miRNAs can be detected from 50 pM to 15 nM with a detection limit of 13.5 pM. The biosensor clearly discriminated the target miRNA from a single base mismatch and non-complementary target oligonucleotides, which could guarantee a high selectivity and specificity. Moreover, the results of real sample assay of the proposed biosensor in human serum showed a good recovery percentage as well as high reproducibility which is promising its future potential use in clinical applications.

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Section: Introductionmentioning

confidence: 99%

Application of Oracet Blue in a novel and sensitive electrochemical biosensor for the detection of microRNA

et al. 2015

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“…The binding constant ( K b ) and binding stoichiometry ( n ) for static quenching can be determined by the following equation, while DOX molecules bind independently to CUR NP surface:

\log [(F_{normal0} - F) / F] = \log K_{normalb} + n \log [Q]

where, F 0 and F are the fluorescence intensities in the absence and presence of the quencher, [ Q ] is the concentration of the quencher, K b is the binding constant, and n is the binding stoichiometry. The values of K b and n were obtained from the intercept and slope of double logarithmic plot of log [( F 0 − F )/ F ] versus log [ Q ] (Figure c) at room temperature.…”

Section: Resultsmentioning

confidence: 99%

Doxorubicin-Anchored Curcumin Nanoparticles for Multimode Cancer Treatment against Human Liver Carcinoma Cells

Venkatesan

Balaji

Sheena

et al. 2015

Part. Part. Syst. Charact.

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1028 wileyonlinelibrary.com www.particle-journal.com www.MaterialsViews.com Curcumin ( Curcuma longa L), a yellow-colored Indian spice, receives immense attention for the prevention and treatment of various cancers. Despite the superlative therapeutic effi cacy, its poor solubility and instability in the aqueous medium hinder the effectiveness of cancer treatment. The novel preparation of curcumin nanoparticles by mechanical grinding of curcumin crystals without any toxic organic solvents is described here for the fi rst time. The surface of curcumin nanoparticles is modifi ed with the negatively charged polyelectrolyte poly(sodium 4-strynesulfonate) through hydrogen bonding, which is the key to increasing the solubility and stability in the aqueous medium. The negative surface charge is exploited to conjugate doxorubicin drug molecule on the surface of curcumin nanoparticles as evidenced by fl uorescence quenching experiments. Doxorubicin-conjugated curcumin nanoparticles have a higher solubility with an enhanced cytotoxic effect toward the human hepatocellular carcinoma cell line by a reactiveoxygen-species-mediated p53-dependent apoptotic pathway. The combination of chemotherapy and photodynamic therapy signifi cantly enhances antitumor activity of doxorubicin-conjugated curcumin nanoparticles, and is expected to be a promising anticancer agent with special reference to human liver carcinoma cells.

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“…The supporting electrolyte was 10 mM of Tris buffer solution (pH 7.4) which was prepared with double distilled water. CV measurements were recorded by keeping the concentration of PIR constant (5.0×10 -5 M) while varying the DNA concentration from 0.0 to 5.0×10 -5 M [37,38].…”

Section: Dna Binding Measurements and Instrumentationmentioning

confidence: 99%

DNA-based electrochemical biosensor using chitosan–carbon nanotubes composite film for biodetection of Pirazon

Pyman

Roshanfekr

Ansari

2020

Eurasian Chem. Commun.

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The interaction of Pirazon (PIR) with calf thymus ds-DNA (double-stranded Deoxyribonucleic acid) in the solution and the immobilized DNA on chitosan-carbon nanotubes composite-modified gold electrode was investigated by electrochemical and UV-Vis spectroscopy techniques. In the solution interactions, spectroscopic results indicate non-intercalative binding of PIR. A competition study with methylene blue, as an intercalative probe, was applied to confirm the binding mode of PIR. PIR showed an oxidation peak at 1.1 V at the bare Au electrode. When ds-DNA was added into the PIR solution, its peak current decreased. Au electrode modified with single wall carbon nanotube (SWCNT) and chitosan (CS) decorated with the ds-DNA was tested to determine PIR content in solution. Electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) were used to characterize the electrochemical properties of the modified electrode. The modified electrode surface has good reproducibility and stability. The modified electrode exhibited linear detection range, 5×10-9 to 5×10-5 M, with a detection limit of 1×10-10 M.

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DNA-binding, DNA cleavage and cytotoxicity studies of two anthraquinone derivatives

Cited by 25 publications

References 60 publications

Application of Oracet Blue in a novel and sensitive electrochemical biosensor for the detection of microRNA

Application of Oracet Blue in a novel and sensitive electrochemical biosensor for the detection of microRNA

Doxorubicin-Anchored Curcumin Nanoparticles for Multimode Cancer Treatment against Human Liver Carcinoma Cells

DNA-based electrochemical biosensor using chitosan–carbon nanotubes composite film for biodetection of Pirazon

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