DNA-Based Identification of<i>Helleborus niger</i>by High-Resolution Melting Analysis

Schmiderer, Corinna; Mader, Eduard; Novak, Johannes

doi:10.1055/s-0030-1249908

Cited by 8 publications

(4 citation statements)

References 7 publications

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“…The first study reporting the use of Bar-HRM to study herbal medicine substitution has recently been reported in a study on species substitution among three medicinal species of Acanthaceae [84]. Bar-HRM has also been used in a number of comparable methodologies [85], such as for authentication of an EU Protected Designation of Origin product made from Lathyrus clymenum [86], olive oil and adulterants [87], subspecies cultivar identification in eggplant [88], identification of closely related species relevant to TDG [89,90], species distinction in Mediterranean pines [91], detection of allergenic hazelnut contamination [92], and processed bean crops [93][94][95].…”

Section: Bar-high Resolution Meltingmentioning

confidence: 99%

DNA Barcoding and Pharmacovigilance of Herbal Medicines

2015

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Pharmacovigilance of herbal medicines relies on the product label information regarding the ingredients and the adherence to good manufacturing practices along the commercialisation chain. Several studies have shown that substitution of plant species occurs in herbal medicines, and this in turn poses a challenge to herbal pharmacovigilance as adverse reactions might be due to adulterated or added ingredients. Authentication of constituents in herbal medicines using analytical chemistry methods can help detect contaminants and toxins, but are often limited or incapable of detecting the source of the contamination. Recent developments in molecular plant identification using DNA sequence data enable accurate identification of plant species from herbal medicines using defined DNA markers. Identification of multiple constituent species from compound herbal medicines using amplicon metabarcoding enables verification of labelled ingredients and detection of substituted, adulterated and added species. DNA barcoding is proving to be a powerful method to assess species composition in herbal medicines and has the potential to be used as a standard method in herbal pharmacovigilance research of adverse reactions to specific products.

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Section: Bar-high Resolution Meltingmentioning

confidence: 99%

DNA Barcoding and Pharmacovigilance of Herbal Medicines

2015

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“…Assays using universal primers targeting the matK gene detected a level of 0.1 % contamination in an admixture of H. niger and an unknown species, but by using primers specific to the common adulterant Veratrum nigrum, the assay detected a level of 0.0005 % contamination of the H. niger/V. nigrum admixture [76].…”

Section: High-resolution Meltingmentioning

confidence: 96%

“…Perhaps surprisingly, HRM has only been applied sparingly to detect adulterants in botanicals. High-resolution melting was used to identify Helleborus niger L. by targeting chloroplast trnL-trnF and matK regions [76]. Although data are limited, assays using universal primers appear to be less sensitive in detecting adulterants than those that target specific adulterant taxa.…”

Section: High-resolution Meltingmentioning

confidence: 99%

DNA-Based Authentication of Botanicals and Plant-Derived Dietary Supplements: Where Have We Been and Where Are We Going?

et al. 2015

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Herbal medicines and botanicals have long been used as sole or additional medical aids worldwide. Currently, billions of dollars are spent on botanicals and related products, but minimal regulation exists regarding their purity, integrity, and efficacy. Cases of adulteration and contamination have led to severe illness and even death in some cases. Identifying the plant material in botanicals and phytomedicines using organoleptic means or through microscopic observation of plant parts is not trivial, and plants are often misidentified. Recently, DNA-based methods have been applied to these products because DNA is not changed by growth conditions unlike the chemical constituents of many active pharmaceutical agents. In recent years, DNA barcoding methods, which are used to identify species diversity in the Tree of Life, have been also applied to botanicals and plant-derived dietary supplements. In this review, we recount the history of DNA-based methods for identification of botanicals and discuss some of the difficulties in defining a specific bar code or codes to use. In addition, we describe how next generation sequencing technologies have enabled new techniques that can be applied to identifying these products with greater authority and resolution. Lastly, we present case histories where dietary supplements, decoctions, and other products have been shown to contain materials other than the main ingredient stipulated on the label. We conclude that there is a fundamental need for greater quality control in this industry, which if not self-imposed, that may result from legislation.

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“…The first study reporting the use of Bar-HRM to evaluate herbal medicine substitution came from a recent investigation of species substitution among three medicinal species of Acanthaceae [ 29 ]. Bar-HRM has also been used in a number of comparable applications [ 34 ], such as for authentication of an EU Protected Designation of Origin product made from Lathyrus clymenum [ 35 ], for the identification of olive oil and adulterants [ 36 ], for subspecies cultivar identification in eggplants [ 37 ], for the identification of closely related species of Sideritis and Helleborus [ 38 , 39 ], for species distinction in Mediterranean pines [ 40 ], for the detection of allergenic hazelnut contamination [ 41 ], and for the identification of processed bean crops [ 42 – 44 ].…”

Section: Introductionmentioning

confidence: 99%

Refining DNA Barcoding Coupled High Resolution Melting for Discrimination of 12 Closely Related Croton Species

et al. 2015

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DNA barcoding coupled high resolution melting (Bar-HRM) is an emerging method for species discrimination based on DNA dissociation kinetics. The aim of this work was to evaluate the suitability of different primer sets, derived from selected DNA regions, for Bar-HRM analysis of species in Croton (Euphorbiaceae), one of the largest genera of plants with over 1,200 species. Seven primer pairs were evaluated (matK, rbcL1, rbcL2, rbcL3, rpoC, trnL and ITS1) from four plastid regions, matK, rbcL, rpoC, and trnL, and the nuclear ribosomal marker ITS1. The primer pair derived from the ITS1 region was the single most effective region for the identification of the tested species, whereas the rbcL1 primer pair gave the lowest resolution. It was observed that the ITS1 barcode was the most useful DNA barcoding region overall for species discrimination out of all of the regions and primers assessed. Our Bar-HRM results here also provide further support for the hypothesis that both sequence and base composition affect DNA duplex stability.

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DNA-Based Identification ofHelleborus nigerby High-Resolution Melting Analysis

Cited by 8 publications

References 7 publications

DNA Barcoding and Pharmacovigilance of Herbal Medicines

DNA Barcoding and Pharmacovigilance of Herbal Medicines

DNA-Based Authentication of Botanicals and Plant-Derived Dietary Supplements: Where Have We Been and Where Are We Going?

Refining DNA Barcoding Coupled High Resolution Melting for Discrimination of 12 Closely Related Croton Species

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