DNA-Based Identification and Chemical Characteristics of Hypnea musciformis from Coastal Sites in Ghana

Abstract

This work reveals new, important insights about the influence of broad spatial variations on the phylogenetic relationship and chemical characteristics of Ghanaian Hypnea musciformis-a carrageenan-containing red seaweed. DNA barcoding techniques alleviate the difficulty for accurate morphological identification. COI barcode sequences of the Ghanaian H. musciformis showed <0.7% intraspecies divergence, indicating no distinct phylogenetic variation, suggesting that they actually belong to the same species. Thus, the spatial distribution of the sampling sites along the coast of Ghana did not influence the phylogenetic characteristics of H. musciformis in the region. The data also showed that the Ghanaian Hypnea sp. examined in this work should be regarded as the same species as the H. musciformis collected in Brazilian Sao Paulo (KP725276) with only 0.8%-1.3% intraspecies divergence. However, the comparison of COI sequences of Ghanaian H. musciformis with the available COI sequence of H. musciformis from other countries showed intraspecies divergences of 0%-6.9% indicating that the COI sequences for H. musciformis in the GenBank may include different subspecies. Although samples did not differ phylogenetically, the chemical characteristics of the H. musciformis differed significantly between different sampling locations in Ghana. The levels of the monosaccharides, notably galactose (20%-30% dw) and glucose (10%-18% dw), as well as the seawater inorganic salt concentration (21-32 mg/L) and ash content (19%-33% dw), varied between H. musciformis collected at different coastal locations in Ghana. The current work demonstrated that DNA-based identification allowed a detailed understanding of H. musciformis phylogenetic characteristics and revealed that chemical compositional differences of H. musciformis occur along the Ghanaian coast which are not coupled with genetic variations among those samples.

Comparison of traditional field retting and Phlebia radiata Cel 26 retting of hemp fibres for fibre-reinforced composites

“…The internally transcribed spacer (ITS) is the DNA situated between the small-subunit rRNA and the large-subunit rRNA genes in the chromosome or the correspondingly transcribed region in the polycistronic rRNA precursor transcript. Extracted DNA (1 μL) was added to a PCR master mix (49 μL) containing 0.5 μM of primers, Phusion HF buffer (F-518), 200 μM dNTPs and 0.5 U Phusion Hot Start II DNA polymerase (#F-549L; Thermo Fisher Scientific, Waltham, USA) (Ale et al 2016 ).…”

DNA barcoding of horn snail Telescopium telescopium (Linnaeus C, 1758) using mt-COI gene sequences

“…The variation in nucleotide composition was studied using GC content analysis. GC content is also varies taxonomically within mitochondrial genome, although all mitochondrial genomes sequenced to date are poor, and widely applied in systematics [25,26]. The mean GC content variation of Indian snails T. telescopium 41.79%, but showed minor variation (41.49 to 42.27 %) are given in Table 1.…”

“…In the previous study, Layton et al, (2014) reported the mean GC content of Canadian molluscs 36.9% (range 24.5-46.5 %), and 37.62 % in north western pacific molluscs varied substantially among mollusan orders [27]. Generally, it is higher in mammals, birds and teleost fish (range 32-46%), following that molluscs (29-40%) and found lowest in nematodes and insects ranging from 15-35% [25].…”

Molecular Approach to the Identification and Phylogenetic Biogeography of Snail <em>Telescopium telescopium</em> Using mt-COI Gene Sequences

“…The variation in nucleotide composition was studied using GC content analysis. GC content is also varies taxonomically within mitochondrial genome, although all mitochondrial genomes sequenced to date are poor, and widely applied in systematics [25,26] were collected in Bay of Bengal waters formed many subclades with the higher value of genetic divergence (1.0-10.5%), these results demonstrated that more intra-subpopulation of horn snail was appeared and diversified in the new mangrove environment shortly and evolved. Similar results of this study were reported by [15], the results of this present study confirmed that the potamidae horn snail's origin and radiation were within the Indo-West Pacific region.…”