DNA barcoding of horn snail Telescopium telescopium (Linnaeus C, 1758) using mt-COI gene sequences

Palanisamy, Satheesh Kumar; Kumar, C. Prasanna; Paramasivam, Purushothaman; Sundaresan, Umamaheswari

doi:10.1016/j.rsma.2020.101109

Cited by 9 publications

(7 citation statements)

References 21 publications

(27 reference statements)

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“…Previous nationalised efforts to barcoding the marine diversity (Lakra et al, 2010;Bineesh et al, 2014;Bamaniya et al, 2015) along with localised efforts to barcode the diversity of Vellar estuary (Khan et al, 2010(Khan et al, , 2011PrasannaKumar et al, 2012;Thirumaraiselvi et al, 2015;Rajthilak et al, 2015;Rahman et al, 2013Rahman et al, , 2019Hemalatha et al, 2016;Sahu et al, 2016;Palanisamy et al, 2020;Manikantan et al, 2020;Thangaraj et al, 2020;Narra et al, 2020) resulted in strengthening the reference library which insured that no ambiguous sequences were present in this study (as all sequences were identified to species level). Identification success was likely due to the use of previously generated references sequences from Indian waters from morphologically verified species and published through reference databases (such as GenBank and BOLD).…”

Section: Discussionmentioning

confidence: 99%

“…In addition, to capture potential genetic variation, including undocumented cryptic diversity, it is important to sequence an adequate number of individuals from across a species range (Weigt et al 2012b). We have made considerable efforts in the past decade as part of the Indian Census of Marine Life (ICoML) to recover barcodes in reasonable numbers of marine phyla including fin & shell fishes, invertebrates (Khan et al, 2010(Khan et al, , 2011PrasannaKumar et al, 2012;Thirumaraiselvi et al, 2015;Rajthilak et al, 2015;Rahman et al, 2013Rahman et al, , 2019Hemalatha et al, 2016;Palanisamy et al, 2020;Manikantan et al, 2020;Thangaraj et al, 2020) and plants (Sahu et al, 2016;Narra et al, 2020) occurring in and around the Vellar estuary. Hence we predict a high rate of success in identification of dietary items of A. maculatus occurring in this environment.…”

Section: Introductionmentioning

confidence: 99%

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Variability in the diet diversity of catfish highlighted through DNA barcoding

PrasannaKumar

Iyyapparajanarasimapallavan

Rahman³

et al. 2020

Preprint

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Identification and quantification of fish diet diversity was the first step in understanding the food web dynamics and ecosystem energetics, where the contribution of DNA barcoding technique has been important. We used DNA barcoding to identify the stomach contents of a euryhaline, benthophagous catfish Ariius maculatus. From 40 catfish stomach items sampled in two different seasons, we barcoded 67 prey items of chordates and macro-invertebrates identified as belonging to 13 species in 4 major phyla (viz., Chordate, Arthropod, Annelida and Mollusca). It is important to note that the mollusc taxa (Meritrix meritrix and Perna viridis) and the species of fish (Stolephorus indicus) could not be found among the gut contents of A. maculatus sampled during the pre- and post-monsoon season, respectively. Among the chordate diets of A. maculatus, Eubleekeria splendens (23.5%) and Stolephorus indicus (23.5%) were the major prey taxa during pre-monsoon season. The hermit crabs forms the major constituents of both pre- and post-monsoon seasons, among invertebrate taxa. Polychaete, Capitella capitata (25.92%) was abundantly consumed invertebrates next to hermit crabs. We noticed that in pre-monsoon A. maculatus was more piscivorous than post-monsoon. As revealed through Kimura-2 parametric pair-wise distance analysis, the diet diversity was relatively higher in post-monsoon. The accumulation curve estimated 57 haplotypes within 14 barcoded species (including the host A. maculatus). Majority of haplotypes were found in Chordates (47.36%) followed by Arthropods (28.07%), Annelids (14.03%) and Mollusca (10.52%), respectively. This study also highlights that there is a growing concern about A. maculatus’s aggressive predation on commercially important stocks of fish and invertebrates. We will continue to expand the coverage of species barcoded in the reference database, which will become more significant as meta- and environmental DNA barcoding techniques become cheaper and prevalent.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Variability in the diet diversity of catfish highlighted through DNA barcoding

PrasannaKumar

Iyyapparajanarasimapallavan

Rahman³

et al. 2020

Preprint

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“…Identifikasi dengan pendekatan molekuler telah banyak dilakukan pada beberapa jenis gastropoda seperti Turbinidae [3], Turbo sp. [13], Telescopium telescopium [14].…”

Section: Pendahuluanunclassified

Analisis Pola Pertumbuhan dan Pendekatan DNA Barcoding untuk Identifikasi Turbo stenogyrus P. Fischer, 1873 (Mollusca: Gastropoda)

et al. 2020

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Pengelolaan dan pelestarian keanekaragaman hayati berawal dari pengetahuan tentang ketersediaan sumberdaya, identifikasi, dan sistematika spesies. Banyaknya spesies Turbinidae dengan berbagai bentuk morfologi dan transformasi bentuk menjadikan identifikasi dengan gen COI sangat penting dalam mempercepat pengungkapan identitas spesies. Penelitian ini dilakukan dengan tujuan menganalisis pola pertumbuhan dan mengidentifikasi gastopoda Turbinidae dengan pendekatan DNA barcode. Hasil identifikasi berdasarkan karakter morfologi menunjukkan bahwa genus Turbo yang diamati adalah dari spesies Turbo stenogyrus. Analisis pola pertumbuhan menunjukkan bahwa pola pertumbuhan T. stenogyrus adalah allometrik negatif. Amplifikasi DNA dengan gen COI menghasilkan panjang sekuen DNA 650 bp dengan hasil identifikasi sesuai dengan pengamatan morfologi yaitu Turbo stenogyrus. Berdasarkan analisis filogenetik dan jarak genetik, sekuen DNA yang dianalisis membentuk clade yang sama dengan spesies Turbo stenogyrus dengan jarak genetik terdekat 4,2%. Analisis genetik melalui gen COI sangat baik dan memberi kemudahan dalam identifikasi spesies gastropoda.

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“…This study used COI gene from mitochondrial DNA as the target gene. The COI genes showed a global bio identification system of animals, a universal primer for a very strong COI gene (Palanisamy et al 2020), and have a greater range of phylogenetic signals than other mitochondrial genes (Sutrisno 2015). This method, however, needs species-specific primers enabling the authentication of products from particular species.…”

Section: Introductionmentioning

confidence: 99%

Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers

Nurilmala¹,

Ajitama²,

Jacoeb³

et al. 2020

Biodiversitas

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Abstract. Nurilmala M, Atjitama Y, Jacob AM, Indarwati AR, Nugraha R. 2020. Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers. Biodiversitas 21: 5650-5656. Shrimp crackers are snacks made from starch with the addition of shrimp and other ingredients. Authentication of shrimp crackers becomes a quality control method in line with its traceability since the morphological characters are not recognized in those products. The failure of the authentication will lead to adulteration of shrimp products. The study aimed to design shrimp-specific polymerase chain reaction (PCR) primers by and used them to authenticate shrimp cracker products to assure their traceability. The primers were designed from cytochrome oxidase subunit I (COI) gene with a GC content of 50% and a melting temperature of 57 °C. The amplicon had a length of 613 bp. Fourteen shrimp crackers having DNA concentrations 0.3 to 8.1 ng/µL were evaluated. Eight out of fourteen DNA samples were successfully amplified using the specific primer and could be visualized on the agarose gel at 500-750 bp. Those DNA samples were identified belonging to shrimp species, namely Litopenaeus vannamei, Metapenaeus ensis, and Fenneropenaeus merguiensis with 95% to 100% identity. Meanwhile, the other six DNA samples underwent PCR using universal primers. One sample was amplified and identified as fish (Sphyraena flavicauda). These results indicate illegal substitution of shrimp using unidentified species occurred in shrimp crackers.

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DNA barcoding of horn snail Telescopium telescopium (Linnaeus C, 1758) using mt-COI gene sequences

Cited by 9 publications

References 21 publications

Variability in the diet diversity of catfish highlighted through DNA barcoding

Variability in the diet diversity of catfish highlighted through DNA barcoding

Analisis Pola Pertumbuhan dan Pendekatan DNA Barcoding untuk Identifikasi Turbo stenogyrus P. Fischer, 1873 (Mollusca: Gastropoda)

Advancing traceability using DNA sequence on seafood product: fraudulence in shrimp crackers

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