DNA-Based Hybridization Chain Reaction for Amplified Bioelectronic Signal and Ultrasensitive Detection of Proteins

Zhang, Bing; Liu, Bingqian; Tang, Dianping; Nießner, Reinhard; Chen, Guonan; Knopp, Dietmar

doi:10.1021/ac3009065

Cited by 384 publications

(160 citation statements)

References 29 publications

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“…Magnetic nanoparticles have been utilized for magnetic capture of aflatoxins in the sample milk giving aid to detoxification. Magnetic nanoparticles are usually functionalized with bio-recognition molecules, such as antibody, aptamers, bioprotein, and carbohydrates for a specific interaction, [14,[27][28][29][30], which allow to aflatoxin detoxification and targeting. Here, we report the utilization of amine-functionalized magnetic nanoparticles for rapid, inexpensive, and effective capture of AFM 1 .…”

Section: Discussionmentioning

confidence: 99%

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

et al. 2017

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Aflatoxins are a kind of mycotoxins that are mostly produced by a group of molds such as Aspergillus flavus and Aspergillus parasiticus. The studies on different parts of Iran showed that AFM 1 infection of milk is higher than European Union's standard. In this study, the specific aptamer with the carboxylic group at the end of 5′ and Fe 3 O 4 nanoparticles with amine groups was synthesized. Morphological and structural qualities of Fe 3 O 4 were determined by the Fourier-transform infrared spectrograph (FTIR), dynamic light scattering, scanning electron microscope, and X-ray diffraction devices. The specificity of the aptamer to AFM 1 was investigated in adjacent of AFM 1 and aflatoxin B 1 . Then, aptamers were attached to nanoparticles to improve synthetic qualities and to ease of its detachment. The attachment was approved by FTIR method. The complex (Fe 3 O 4 -APT) was then added to infected milk and after the proper time was detached from the milk using a magnet. The remained amount of AFM 1 was attained in milk using high-performance liquid chromatography. Our result showed that this method for aflatoxin detoxification is much more effective than conventional methods based on recognition of AFM 1 and their concentration in infected milk. This method is more applicable, faster, and cheaper highlighting its application in milk factories. Graphical abstractKeywords Aflatoxin · Aptamer · Fe 3 O 4 nanoparticle · Detoxification · Mycotoxin

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Section: Discussionmentioning

confidence: 99%

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

et al. 2017

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“…A remarkable limit of detection for IgG of 0.1 fg/mL À1 was obtained (Fig. 5, Zhang et al 2012a). (Zhou et al 2013) 4 Nucleic Acids for Both Binding and Amplified Detection…”

Section: Nanoparticles For Ultrasensitive Assay Developmentmentioning

confidence: 92%

DNA for Non-nucleic Acid Sensing

2015

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“…[15] On the other hand, since introduced by Dirks and Pierce, [16] hybridization chain reaction (HCR) has been widely applied in analysis as an effective signal amplification. [17] And in comparison to other signal amplification strategies, HCR is an enzyme-free process with no need for expensive enzymes.…”

Section: Introductionmentioning

confidence: 99%

Microfluidic Paper‐Based Analytical Device for Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of Hybridization Chain Reaction

Liu

Zhang

et al. 2017

ChemElectroChem

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In this paper, a microfluidic paper-based analytical device is presented for sensitively detecting peptides based on the specific recognition of aptamer and the amplification strategy of hybridization chain reaction (HCR). The aptamer/initiation strand (AP/IS) was designed for the specific target recognition, and the presence of the target peptide could release the initiating sequence from the AP/IS. The released IS could trigger the HCR between two hairpin probes, which could complete the detection for the targets indirectly when the electrochemiluminescence (ECL) probe (Ru(phen) 3 2 + ) is introduced into the double-strand DNA grooves,which could produce a significantly amplified ECL signal output. Mucin-1 was employed as a model peptide, and the detection limit of the proposed assay is calculated as 8.33 pMwith a range from 25 pM to 50 nM. The novel sensing platform can be widened for the detection of other kinds of peptides as well as potentials used for sensitively detecting other biomolecules, and even cells.

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DNA-Based Hybridization Chain Reaction for Amplified Bioelectronic Signal and Ultrasensitive Detection of Proteins

Cited by 384 publications

References 29 publications

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

Aflatoxin M1 detoxification from infected milk using Fe3O4 nanoparticles attached to specific aptamer

DNA for Non-nucleic Acid Sensing

Microfluidic Paper‐Based Analytical Device for Sensitive Detection of Peptides Based on Specific Recognition of Aptamer and Amplification Strategy of Hybridization Chain Reaction

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