DNA base sequence variability in<i>katF</i>(putative sigma factor) gene of<i>Escherichia coli</i>

Ivanova, Anna; Renshaw, Mary; Guntaka, Ramareddy V.; Cohen, Gerald

doi:10.1093/nar/20.20.5479

Cited by 62 publications

(44 citation statements)

References 3 publications

(1 reference statement)

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“…1B). This open reading frame encoded a protein of 330 amino acids with a predicted molecular weight of 38 kDa which showed levels of identity of 99 and 95% with the KatF (RpoS and as) proteins of E. coli and Shigellaflexneri, respectively (15,24,39,42). Thus, this protein was henceforth referred to as S. typhimurium as.…”

Section: Resultsmentioning

confidence: 99%

The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence, and regulation of spvR and spvABCD virulence plasmid genes

et al. 1994

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Section: Resultsmentioning

confidence: 99%

The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence, and regulation of spvR and spvABCD virulence plasmid genes

et al. 1994

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“…The variability correlated with phenotypic differences in the expression of RpoS-controlled genes (Ivanova et al, 1992). Such strain differences may explain the considerable variation in the CFA synthase level observed in several strains of E. coli K-12 expressing various plasmid and phage cfa clones (Grogan & Cronan, 1984).…”

Section: Discussionmentioning

confidence: 99%

The formation of cyclopropane fatty acids in Salmonella enterica serovar Typhimurium

Kim

et al. 2005

Microbiology

110

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The formation of cyclopropane fatty acid (CFA) and its role in the acid shock response in Salmonella enterica serovar Typhimurium (S. typhimurium) was investigated. Data obtained by GC/MS demonstrated that the CFA level in S. typhimurium increased upon its entry to the stationary phase, as in other bacteria. The cfa gene encoding CFA synthase was cloned, and mutants of the cfa gene were constructed by allelic exchange. A cfa mutant could not produce CFA and was sensitive to low pH. Introduction of a functional cfa gene into a cfa mutant cell made the mutant convert all unsaturated fatty acids to CFAs and partially restored resistance to low pH. Interestingly, the alternative sigma factor RpoS, which was induced during the stationary phase, affected the production of C19 CFA but not C17 CFA. Western blotting analysis showed that the increase in expression of CFA synthase at early stationary phase was due to the alternative sigma factor RpoS.

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“…However, amplified Lac ϩ cells take 3-5 days to form a colony (Table 2; Hastings et al 2000). Thus, changes in the population RESULTS size 5 days after plating (day 7 in Figure 1, at which point rpoS ϩ shows growth and the rpoS mutant does not) An rpoS mutation decreases stationary-phase mutation and amplification: An rpoS null allele (Loewen and do not affect amplified colony yield until at least day 8, whereas the difference in amplified colony yield in rpoS Triggs 1984; Ivanova et al 1992) decreases Lac ϩ colony yield substantially ( Figure 1A, "total" values). Multiis clear even by day 5.…”

Section: Methodsmentioning

confidence: 99%

“…rpoS regulatory network induced in response to various envicodon 33 in SMR4562 is TTG, encoding leucine, instead ronmental stresses, including the onset of stationary of the CAG, encoding glutamine, in MG1655. This cophase (Loewen and Hengge-Aronis 1994), is required don varies in laboratory strains (glutamine or an amber for most stationary-phase point mutation and amplificacodon are common; Ivanova et al 1992;Visick and tion in the Lac system ( Figure 1; Table 2; results re- Clarke 1997;Atlung et al 2002). However, this variant ported in the text).…”

Section: Methodsmentioning

confidence: 99%

General Stress Response Regulator RpoS in Adaptive Mutation and Amplification inEscherichia coli

Lombardo¹,

Aponyi²,

Rosenberg³

2004

Genetics

131

183

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Microbial cells under growth-limiting stress can generate mutations by mechanisms distinct from those in rapidly growing cells. These mechanisms might be specific stress responses that increase mutation rates, potentially altering rates of evolution, or might reflect non-stress-specific processes in rare growing cells.In an Escherichia coli model system, both frameshift reversion mutations and gene amplifications occur as apparent starvation-induced mutations. Whereas frameshift reversion ("point mutation") requires recombination proteins, the SOS response, and error-prone DNA polymerase IV (DinB), amplification requires neither SOS nor pol IV. We report that both point mutation and amplification require the stationaryphase and general stress response transcription factor RpoS ( S ). Growth-dependent mutation does not. Alternative interpretations are excluded. The results imply, first, that point mutation and amplification are stress responses that occur in differentiated stationary-phase (not rare growing) cells and, second, that transient genetic instability, producing both point mutation and genome rearrangement, may be a previously unrecognized component of the RpoS-dependent general stress response.

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DNA base sequence variability inkatF(putative sigma factor) gene ofEscherichia coli

Cited by 62 publications

References 3 publications

The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence, and regulation of spvR and spvABCD virulence plasmid genes

The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence, and regulation of spvR and spvABCD virulence plasmid genes

The formation of cyclopropane fatty acids in Salmonella enterica serovar Typhimurium

General Stress Response Regulator RpoS in Adaptive Mutation and Amplification inEscherichia coli

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