The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence, and regulation of spvR and spvABCD virulence plasmid genes

Kowarz, L.; Coynault, C; Robbe‐Saule, Véronique; Norel, Françoise

doi:10.1128/jb.176.22.6852-6860.1994

Cited by 144 publications

(175 citation statements)

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“…The 1.1-kb HindIIISalI fragment carrying the cat cartridge from pAMPCm (37) was cloned into the HindIII-XhoI restriction sites of pVKKatF to obtain pVKKatFCm. pUCK3Km contains the Salmonella serotype Typhimurium rpoS region in which the 0.8-kb HpaI-PstI fragment in rpoS has been replaced by the 1.3-kb HincII fragment encoding the kanamycin resistance gene (24). Strains were grown at 37°C in Luria-Bertani medium (LB) (39).…”

Section: Methodsmentioning

confidence: 99%

“…pVK100 is a mobilizable, low-copy-number cloning vector (23). pVKKatF contains the 2-kb SalI fragment carrying the Salmonella serotype Typhimurium rpoS gene from pSTK5 (24) cloned into the SalI restriction site of pVK100. The 1.1-kb HindIIISalI fragment carrying the cat cartridge from pAMPCm (37) was cloned into the HindIII-XhoI restriction sites of pVKKatF to obtain pVKKatFCm.…”

Section: Methodsmentioning

confidence: 99%

“…S also regulates chromosomal genes required for the colonization of Peyer's patches (6,30) and for persistence in infected mice (24). Salmonella serotype Typhi does not contain a virulence plasmid, and the role of rpoS in the virulence of this serotype is not known yet.…”

mentioning

confidence: 99%

“…RpoS plays a key role in the virulence of Salmonella in mice (5,6,8,24,30,31). Salmonellae are enteric pathogens that cause a wide range of host-and serotype-specific illnesses, including gastroenteritis and enteric fever.…”

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confidence: 99%

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Characterization of the RpoS Status of Clinical Isolates of Salmonella enterica

Robbe‐Saule

Algorta

Rouilhac

et al. 2003

Appl Environ Microbiol

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The stationary-phase-inducible sigma factor, S (RpoS), is the master regulator of the general stress response in Salmonella and is required for virulence in mice. rpoS mutants can frequently be isolated from highly passaged laboratory strains of Salmonella. We examined the rpoS status of 116 human clinical isolates of Salmonella, including 41 Salmonella enterica serotype Typhi strains isolated from blood, 38 S. enterica serotype Typhimurium strains isolated from blood, and 37 Salmonella serotype Typhimurium strains isolated from feces. We examined the abilities of these strains to produce the S protein, to express RpoS-dependent catalase activity, and to resist to oxidative stress in the stationary phase of growth. We also carried out complementation experiments with a cloned wild-type rpoS gene. Our results showed that 15 of the 41 Salmonella serotype Typhi isolates were defective in RpoS. We sequenced the rpoS allele of 12 strains. This led to identification of small insertions, deletions, and point mutations resulting in premature stop codons or affecting regions 1 and 2 of S , showing that the rpoS mutations are not clonal. Thus, mutant rpoS alleles can be found in freshly isolated clinical strains of Salmonella serotype Typhi, and they may affect virulence properties. Interestingly however, no rpoS mutants were found among the 75 Salmonella serotype Typhimurium isolates. Strains that differed in catalase activity and resistance to hydrogen peroxide were found, but the differences were not linked to the rpoS status. This suggests that Salmonella serotype Typhimurium rpoS mutants are counterselected because rpoS plays a role in the pathogenesis of Salmonella serotype Typhimurium in humans or in the transmission cycle of the disease.

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Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Characterization of the RpoS Status of Clinical Isolates of Salmonella enterica

Robbe‐Saule

Algorta

Rouilhac

et al. 2003

Appl Environ Microbiol

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“…Also known as 38 or s , RpoS regulates expression of Ϸ10% of Escherichia coli genes (3), including katE and xthA, which encode hydroperoxidase II catalase and exonuclease III, respectively (4,5). In Salmonella enterica serovar Typhimurium, RpoS plays a major role in virulence by controlling expression of the spv plasmid genes (6,7), which govern Salmonella's growth rate in deep organs and are required for systemic infection in mice (8)(9)(10).…”

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confidence: 99%

The PhoP/PhoQ two-component system stabilizes the alternative sigma factor RpoS in Salmonella enterica

Latifi²,

Bougdour³

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

110

122

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The sigma factor RpoS regulates the expression of many stress response genes and is required for virulence in several bacterial species. We now report that RpoS accumulates when Salmonella enterica serovar Typhimurium is growing logarithmically in media with low Mg 2؉ concentrations. This process requires the twocomponent regulatory system PhoP͞PhoQ, which is specifically activated in low Mg 2؉ . We show that PhoP controls RpoS protein turnover by serving as a transcriptional activator of the iraP (yaiB) gene, which encodes a product that enhances RpoS stability by interacting with RssB, the protein that normally delivers RpoS to the ClpXP protease for degradation. Mutation of the phoP gene rendered Salmonella as sensitive to hydrogen peroxide as an rpoS mutant after growth in low Mg 2؉ . In Escherichia coli, low Mg 2؉ leads to only modest RpoS stabilization, and iraP is not regulated by PhoP͞PhoQ. These findings add the sigma factor RpoS to the regulatory proteins and two-component systems that are elevated in a PhoP͞PhoQ-dependent fashion when Salmonella face low Mg 2؉ environments. Our data also exemplify the critical differences in regulatory circuits that exist between the closely related enteric bacteria Salmonella and E. coli.IraP ͉ magnesium ͉ Escherichia coli

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Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs

Wang

Wei

2011

J. Basic Microbiol.

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Vibrio alginolyticus, one of the most important opportunistic pathogens, can be detected in human being and marine animals. Like other bacteria, V. alginolyticus is able to adapt to a variety of stressful environmental changes. The alternate sigma factor RpoS, which is a regulator during stationary phase, plays an important role in surviving under these stressful situations in many bacteria. Sequence analysis reveals a 990 bp open reading frame which is predicted to encode a 330-amino-acid protein with 68% to 96% overall identity to other reported sequences. To study the function of rpoS, the rpoS gene of V. alginolyticus VIB283 was cloned and an rpoS mutant was constructed by homologous recombination. Comparison of the study result of the wild type and the mutant showed that the mutant was more sensitive to stress conditions such as high osmolarity, oxidative stress, heat shock, and long-term starvation and that the LD(50) of the mutant strain to the zebra fish was about 2.8 times as that of the control strain. In addition, the SDS-PAGE analysis indicated that the outer membrane proteins (OMPs) existed great differences.

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The Salmonella typhimurium katF (rpoS) gene: cloning, nucleotide sequence, and regulation of spvR and spvABCD virulence plasmid genes

Abstract: The spv region of SalmoneUla virulence plasmids is essential for the development of a systemic infection in mice.

Cited by 144 publications

References 43 publications

Characterization of the RpoS Status of Clinical Isolates of Salmonella enterica

Characterization of the RpoS Status of Clinical Isolates of Salmonella enterica

The PhoP/PhoQ two-component system stabilizes the alternative sigma factor RpoS in Salmonella enterica

Susceptibility of a Vibrio alginolyticus rpoS mutant to environmental stresses and its expression of OMPs

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