DNA Barcoding of Two Medicinal Plants Using Molecular Markers

El-Atroush, H.

doi:10.21608/eajbsc.2020.85995

Cited by 2 publications

(2 citation statements)

References 19 publications

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“…A DNA barcode was incorporated to address the challenge of chemical plasticity, as the genetic makeup of a particular species should be more stable under various environmental conditions [ 52 ]. In plants, the establishment and refinement of DNA barcodes have been more challenging due to the distinct genetic diversity among different species [ 53 ].…”

Section: Resultsmentioning

confidence: 99%

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

et al. 2022

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Cinnamomum verum (Lauraceae), also known as “true cinnamon” or “Ceylon cinnamon” has been widely used in traditional folk medicine and cuisine for a long time. The systematics of C. verum presents some difficulties due to genetic variation and morphological similarity between other Cinnamomum species. The present work aimed to find chemical and molecular markers of C. verum samples from the Amazon region of Brazil. The leaf EOs and the genetic material (DNA) were extracted from samples cultivated and commercial samples. The chemical composition of the essential oils from samples of C. verum cultivated (Cve1-Cve5) and commercial (Cve6-c-Cv9-c) was grouped by multivariate statistical analysis of Principal Component Analysis (PCA). The major compounds were rich in benzenoids and phenylpropanoids, such as eugenol (0.7–91.0%), benzyl benzoate (0.28–76.51%), (E)-cinnamyl acetate (0.36–32.1%), and (E)-cinnamaldehyde (1.0–19.73%). DNA barcodes were developed for phylogenetic analysis using the chloroplastic regions of the matK and rbcL genes, and psbA-trnH intergenic spacer. The psbA-trnH sequences provided greater diversity of nucleotides, and matK confirmed the identity of C. verum. The combination of DNA barcode and volatile profile was found to be an important tool for the discrimination of C. verum varieties and to examine the authenticity of industrial sources.

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Section: Resultsmentioning

confidence: 99%

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

et al. 2022

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“…DNA barcoding has widely been viewed as a neotaxonomic tool with various other applications, e.g., identification of herbal medicinal plants/products essential for human health (Yu et al 2021). This method not only aids in the classification of organisms but also provides genetic information for ancestral inheritance and the identification of new species (El-Atroush et al 2015). In 2009, during the third International Barcode of Life Conference, the combination of ribulose-1,5-bisphosphate carboxylase (rbcL) + maturase K (matK) loci was recommended as the core DNA sequence for plants, whereas Internal Transcribed Spacer (ITS) and trnH-psbA as complementary sequences (Kress et al 2005;Group 2009).…”

Section: Introductionmentioning

confidence: 99%

Exploring the Medicinal Flora of District Musakhel, Pakistan: A DNA Barcoding and Ethnobotanical Investigation

Kanwal,

Irfan,

Ahmed

et al. 2024

Ethnobot. Res. App.

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Background: District Musakhel in Pakistan is known for its unique geographical and climatic conditions, fostering diverse flora and fauna, including unexplored medicinal plants. This study aims to systematically categorize these medicinal plant species using traditional taxonomic methods and advanced DNA barcoding techniques. Methods:The methodology included the ethnobotanical study and plant sampling, DNA extraction, PCR amplification of rbcL and ITS2 regions, sequencing, and subsequent taxonomic identification using BLAST and Phylogenetic analysis via the Neighbor-Joining method. Results:The rbcL barcode exhibited efficacy in discerning taxonomic relationships at the familial and generic levels, while the ITS2 barcode excelled in elucidating species-specific variations, albeit encountering challenges in clustering species within families. Nevertheless, the most precise taxonomic resolution was achieved by combining both barcodes. Furthermore, Phylogenetic tree analysis confirmed the effectiveness of the rbcL over ITS2 and the combined barcoding approach. The rbcL tree displayed robust performance, with 76.9% of nodes surpassing the 50 bootstrap value threshold and successfully resolving 85% of the studied species. The ITS2 tree, while weaker, still resolved 65% of the species. The combined analysis, integrating rbcL and ITS2 data, achieved a 58% success rate in species resolution. This research explored the ethnobotanical features, highlighting that Plantago major had the highest utilization among the species surveyed. Cirsium shansiense showed the greatest use value (0.37), while Ficus palmata and Solanum xanthocarpum had the highest Relative Frequency of Citation (RFC) values. Conclusions:In a nutshell, this study serves as a foundational contribution to thoroughly document, precisely identify, and conserve the priceless indigenous medicinal plant species in District Musakhel.

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DNA Barcoding of Two Medicinal Plants Using Molecular Markers

Cited by 2 publications

References 19 publications

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

Essential Oil Chemotypes and Genetic Variability of Cinnamomum verum Leaf Samples Commercialized and Cultivated in the Amazon

Exploring the Medicinal Flora of District Musakhel, Pakistan: A DNA Barcoding and Ethnobotanical Investigation

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