DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species

Gutierrez, Maria A.; Lopez, Richard Onalbi Hoyos; Ramos, Ana Teresa; Vélez, Iván Darío; Gomez, R.V.S.; Arrivillaga-Henríquez, Jazzmin; Uribe, Sandra

doi:10.1016/j.actatropica.2021.105983

Cited by 12 publications

(5 citation statements)

References 83 publications

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“…2). This pattern has been seen in studies with a wide geographic distribution of the analyzed species [16, 19,39,[54][55] or when clear geographic barriers are assessed, such as Amazonian riverbanks [14,56] and caves in Thailand [18]. In the present study, a remarkable case comprising specimens of Pi.…”

Section: Discussionsupporting

confidence: 76%

Improving the COI DNA barcoding library for Neotropical phlebotomine sand flies (Diptera, Psychodidae)

Posada-López

Vélez²,

Uribe³

2023

Preprint

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Background: Sand fly species are traditionally identified using morphological traits, thought this method is hampered by the presence of cryptic species. DNA barcoding is a widely used tool in the case of insects of medical importance, where it is necessary to know quickly which species are present in a transmission area. Here, we assess the usefulness of COI DNA barcoding as a practical tool for species identification, correct assignment of isomorphic females, and to evaluate the detection of cryptic diversity that occurs in the same species. Results: A fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was used to generate 156 new barcode sequences for sand flies from different countries of the Neotropical region, mainly Colombia, which had been identified morphologically as 43 species. The sequencing of the COI gene allowed the detection of cryptic diversity within species and correctly associated isomorphic females with males identified by morphology. The maximum intraspecific genetic distances ranged from 0 to 8.32% and 0 to 8.92% using uncorrected p distances and the K2P model, respectively. The minimum interspecific distance (nearest neighbor) for each species ranged from 1.5 to 14.14% and 1.51 to 15.7% using p and K2P distances, respectively. Three species had more than 3% maximum intraspecific distance: Psychodopygus panamensis, Micropygomyia cayennensis cayennensis, and Pintomyia evansi. They also were splitted into at least two MOTUs each, using different species delimitation algorithms. Regarding interspecific genetic distances, the species of the genera Nyssomyia and Trichophoromyia generated values lower than 3% (except Ny. ylephiletor and Ny. trapidoi). However, the maximum intraspecific distances did not exceed these values, indicating the presence of a barcode gap despite their proximity. Also, nine sand fly species were DNA barcoded for the first time: Evandromyia georgii, Lutzomyia sherlocki, Ny. ylephiletor, Ny. yuilli pajoti, Psathyromyia punctigeniculata, Sciopemyia preclara, Trichopygomyia triramula, Trichophoromyia howardi, and Th. velezbernali. Conclusions: The COI DNA barcode analysis allowed the correct delimitation of several Neotropical sand fly species from South and Central America and raised questions about the presence of cryptic species for some taxa, which should be further assessed.

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Section: Discussionsupporting

confidence: 76%

Improving the COI DNA barcoding library for Neotropical phlebotomine sand flies (Diptera, Psychodidae)

Posada-López

Vélez²,

Uribe³

2023

Preprint

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“…In the first four cases, the detection of these genetic lineages may be associated with microevolutionary processes due to isolation by distance and geographic barriers (e.g., Andean region) since these four species presented clusters related to the geographic locations where they were sampled (Figure 2). This pattern has been seen in studies with a wide geographic distribution of the analyzed species [16,19,39,[54][55] or when clear geographic barriers are assessed, such as Amazonian riverbanks [14,56] and caves in Thailand [18]. In the present study, a remarkable case comprising specimens of Pi.…”

Section: Discussionsupporting

confidence: 72%

Improving theCOIDNA barcoding library for Neotropical phlebotomine sand flies (Diptera, Psychodidae)

Posada-López

Rodrigues

Vélez

et al. 2022

Preprint

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A fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was used to generate 156 new barcode sequences for sand flies from different countries of the Neotropical region, mainly Colombia, which had been identified morphologically as 43 species. The sequencing of the COI gene allowed the detection of cryptic diversity within species and correctly associated isomorphic females with males identified by morphology. The maximum intraspecific genetic distances ranged from 0 to 8.32% and 0 to 8.92% using uncorrected p distances and the K2P model, respectively. The minimum interspecific distance (nearest neighbor) for each species ranged from 1.5 to 14.14% and 1.51 to 15.7% using p and K2P distances, respectively. Three species had more than 3% maximum intraspecific distance: Psychodopygus panamensis, Micropygomyia cayennensis cayennensis, and Pintomyia evansi. They also were splitted into at least two MOTUs each, using different species delimitation algorithms. Regarding interspecific genetic distances, the species of the genera Nyssomyia and Trichophoromyia generated values lower than 3% (except Ny. ylephiletor and Ny. trapidoi). However, the maximum intraspecific distances did not exceed these values, indicating the presence of a barcode gap despite their proximity. Also, nine sand fly species were DNA barcoded for the first time: Evandromyia georgii, Lutzomyia sherlocki, Ny. ylephiletor, Ny. yuilli pajoti, Psathyromyia punctigeniculata, Sciopemyia preclara, Trichopygomyia triramula, Trichophoromyia howardi, and Th. velezbernali. Thus, the COI DNA barcode analysis allowed the correct delimitation of several Neotropical sand fly species from South and Central America and raised questions about the presence of cryptic species for some taxa, which should be further assessed.

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“…Specifically, Lu. longipalpis is considered a complex of species, consisting in at least 8 lineages, that are present in the Americas, from Mexico to Argentina, in tropical dry forests, arid and semi-arid areas and in very humid environments such as the riverside of the Amazon River, being considered the main vector of L. infantum in the region (Flórez et al, 2006;Soussa-Paula et al, 2020;Gutierrez et al, 2021). The control measurements for this vector, and in general for the phlebotomine sand flies that exist in Colombia, are based on community education at the risk areas, modification of the sanitary conditions of the houses, use of protective clothing and repellent (Ministerio de salud, 2006), taking into account that there are still no vaccines against leishmaniasis that are effective in humans (Lage et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

Win by Quantity: a Striking Rickettsia-Bias Symbiont Community Revealed by Seasonal Tracking in the Whitefly Bemisia tabaci

et al. 2020

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Molecular Detection of Endosymbionts in Phlebotomines and Temperature Preference Estimation and Its Relation with Microbiota with Emphasis onLutzomyia longipalpisDue to climate change, there is an increase in tropical diseases such as leishmaniasis, transmitted by some species of the genus Lutzomyia, as Lutzomyia longipalpis, vector of Leishmania infantum in America. In addition, the microbiota of insects is known to play a role in their immunity, directly impacting their vector competence to transmit pathogens.This justifies the need to explore the composition of the microbiota, the presence of endosymbionts and their potential relationship with temperature variations in Lu.longipalpis. For this reason, the molecular detection of Arsenophonus was conducted in populations of wild phlebotomines of Lu. longipalpis, Pintomyia evansi and Psychodopygus panamensis from Colombia. Subsequently, with a device with temperature gradient "MB-Thermocline", it was evaluated the temperature preference of Lu. longipalpis, Pi. evansi while populations of Aedes aegypti were used as a control in the assay. The PCR results showed the presence of Arsenophonus and interspecific differences (p-value < 0.05) were observed between phlebotomines, specifically between 25 °C and 31 °C where there was a greater abundance of Pi. evansi found in such compartments, however both species showed a marked preference towards the temperature of 21-23 °C, while Ae. aegypti prefered temperatures between 27-29 °C.Representative groups of Lu. longipalpis that presented temperature preference (phenotypes) in each compartment of the device, were used to perform an analysis of the microbiota using New Generation Sequencing techniques. The analysis of the microbiota of these groups shows that the communities have a significantly different taxonomic structure between temperature ranges (p-value < 0.013), the most abundant genera were

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DNA barcoding of Lutzomyia longipalpis species complex (Diptera: Psychodidae), suggests the existence of 8 candidate species

Cited by 12 publications

References 83 publications

Improving the COI DNA barcoding library for Neotropical phlebotomine sand flies (Diptera, Psychodidae)

Improving the COI DNA barcoding library for Neotropical phlebotomine sand flies (Diptera, Psychodidae)

Improving theCOIDNA barcoding library for Neotropical phlebotomine sand flies (Diptera, Psychodidae)

Win by Quantity: a Striking Rickettsia-Bias Symbiont Community Revealed by Seasonal Tracking in the Whitefly Bemisia tabaci

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