2022
DOI: 10.1101/2022.12.14.520486
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Improving theCOIDNA barcoding library for Neotropical phlebotomine sand flies (Diptera, Psychodidae)

Abstract: A fragment of the mitochondrial cytochrome c oxidase subunit I (COI) gene was used to generate 156 new barcode sequences for sand flies from different countries of the Neotropical region, mainly Colombia, which had been identified morphologically as 43 species. The sequencing of the COI gene allowed the detection of cryptic diversity within species and correctly associated isomorphic females with males identified by morphology. The maximum intraspecific genetic distances ranged from 0 to 8.32% and 0 to 8.92% u… Show more

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(2 citation statements)
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“…COI ‐barcodes seem to be a useful tool in detecting cryptic diversity when analysing populations of this taxon from different geographical areas and biomes. This molecular marker has been widely used to uncover cryptic diversity within sand flies, especially because researchers from several regions can compare their findings with previously deposited sequences, which increases the detection of possible cryptic species in broad geographical scale analysis (Posada López et al, 2023; Rodrigues & Galati, 2023). The single‐locus species delimitation analyses based on COI sequences were effective for an unambiguous identification with their most closely related species— P. claustrei .…”
Section: Discussionmentioning
confidence: 99%
See 1 more Smart Citation
“…COI ‐barcodes seem to be a useful tool in detecting cryptic diversity when analysing populations of this taxon from different geographical areas and biomes. This molecular marker has been widely used to uncover cryptic diversity within sand flies, especially because researchers from several regions can compare their findings with previously deposited sequences, which increases the detection of possible cryptic species in broad geographical scale analysis (Posada López et al, 2023; Rodrigues & Galati, 2023). The single‐locus species delimitation analyses based on COI sequences were effective for an unambiguous identification with their most closely related species— P. claustrei .…”
Section: Discussionmentioning
confidence: 99%
“…It is also noteworthy that Rondônia populations were better sampled than the others in our study, and this may have influenced the discovery of greater diversity in this state. Unequal sampling efforts may have a major influence on the discovery of cryptic diversity, and DNA barcoding datasets containing population/species with a greater number of sequences tend to split into multiple MOTUs (Pinto et al, 2015; Posada López et al, 2023).…”
Section: Discussionmentioning
confidence: 99%