DNA barcoding identification of Perciform fishes of the Arabian Gulf commercially harvested in Qatar

Alam, Samsul; Alkuwari, Aisha Dasmal; Alhashimi, Noura; Al-Malki, Ameena

doi:10.21608/ejabf.2020.146443

Cited by 2 publications

(3 citation statements)

References 25 publications

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“…This is consistent with the patterns observed in other fish barcoding studies (Ward et al, 2005;Lakra et al, 2011;Zhang, 2011;Keskin & Atar, 2013;Bingpeng et al, 2018;Wang et al, 2018;Thu et al, 2019). The mean intraspecific divergence found in Tunisian waters (K2P = 0.52%) was higher than that observed in previous studies (Ward et al, 2005;Hubert et al, 2008;Steinke et al, 2009;Valdez-Moreno et al, 2009;Lakra et al, 2011;Zhang, 2011;Zhang & Hanner, 2012;Keskin & Atar, 2013;Bingpeng et al, 2018;Thu et al, 2019;Alam et al, 2020) and much lower than the 0.6% reported by Lara et al (2010). Indeed, it is still much lower than the 2% needed to annotate the existence of new cryptic species.…”

Section: Discussionsupporting

confidence: 90%

“…there was a 13.2-fold more sequence difference among congeneric species than conspecific individuals, which is relatively similar to the 13.9-fold observed in the Canadian Atlantic marine fishes (McCusker et al, 2013). However, it is clearly lower than the 22-fold by Saccone et al (1999) and Lakra et al (2011), 25-fold by Ward et al (2005), 26.2-fold by Kenchington et al (2017) and 31-fold by Bingpeng et al (2018), but it falls within the range of 10.4-and 66.7-fold reported by Asgharian et al (2011) and 46.35% by Alam et al (2020).…”

Section: Discussionsupporting

confidence: 79%

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DNA barcoding of commercially relevant marine fish species in Tunisian waters

et al. 2022

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DNA barcoding based on the cytochrome c oxidase subunit I (COI) gene in mitochondrial DNA has been adopted as a global biological identification system for animals due to its accuracy compared with other classical taxonomic methods. The objective of this study was to establish a reference library with generated barcodes. A total of 84 fish specimens belonging to 37 commercially important marine fish species, representing five orders, 14 families and 30 genera, were sampled along the Tunisian coast and barcoded for the first time, obtaining 637 bp sequences. The average Kimura 2-parameter (K2P) distances within species, genera and families were 0.52, 6.86 and 14.60%, respectively. The Maximum likelihood (ML) tree revealed distinct clusters in concurrence with the taxonomic status of the species. Our results confirmed the authentication of the barcode approach for the identification of the species examined and provide valuable information that would help ichthyologists to achieve better monitoring, conservation and management of fisheries in Tunisia.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionsupporting

confidence: 79%

DNA barcoding of commercially relevant marine fish species in Tunisian waters

et al. 2022

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“…DNA barcoding is thought to be a method for species discrimination, which helps in comprehending the diversity within an ecosystem and assessing genetic variability between them ( Rajkumar et al ., 2015 ; Abbas et al ., 2017 ; Ghouri et al ., 2020 ). Previous research has used the COI gene to confirm the taxonomic status of many Acanthopagrus species ( Hsu et al ., 2011 ; Wu et al ., 2018 ; Alam et al ., 2020 ; Liu et al ., 2021 ; Pan et al ., 2021 ; Islam et al ., 2022 ; Pombo-Ayora et al ., 2022 ). The host’s morphological identification was established by comparing COI sequences to comparable organisms.…”

Section: Discussionmentioning

confidence: 99%

Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia

Alojayri,

Al-Quraishy,

Al-Shaebi

et al. 2024

Helminthologia

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Summary Ectoparasites, particularly monogeneans, negatively affect fish health and growth. This study identified monogenean parasites in the twobar seabream, Acanthopagrus bifasciatus (Sparidae), inhabited the Arabian Gulf (Saudi Arabia). Following that, forty A. bifasciatus fish samples were visually examined for monogeneans. Parasite species were collected from the gills and then analyzed morphometrically, morphologically, and molecularly using the partial regions of the large subunit of ribosomal RNA (28S rRNA) and mitochondrial cytochrome C oxidase subunit I (COI) genes. Fish species were also identified using a DNA barcoding approach based on the COI gene. The monogenean species of Diclidophora merlangi (Diclidophoridae) were found in 45% of the fish species studied. The generic features of the Diclidophora genus distinguish this species. This species discriminated itself from congeners by having a muscular bulb with 17 grooved and recurved hooks, 218±10 (184–267) post-ovarian testes, and four pairs of pedunculated clamps of relative sizes. Partial 28S rRNA sequencing from monogeneans revealed that they grouped with members of the genus Diclidophora, forming a monophyletic group that supported the morphological descriptions. Molecular identification revealed that D. merlangi has a unique barcode made up of a COI sequence. The host identity was established as A. bifasciatus based on the COI gene sequences. Furthermore, a molecular phylogenetic study was performed to determine the phylogenetic affinity of parasite species and fish hosts. This study on Diclidophora species is considered the first record of this genus in the examined area.

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DNA barcoding identification of Perciform fishes of the Arabian Gulf commercially harvested in Qatar

Cited by 2 publications

References 25 publications

DNA barcoding of commercially relevant marine fish species in Tunisian waters

DNA barcoding of commercially relevant marine fish species in Tunisian waters

Morphological and genetic identification of the gill monogenean parasite (Diclidophora merlangi) that infects Twobar Seabream Fish (Acanthopagrus bifasciatus) in the Arabian Gulf, Saudi Arabia

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