DNA barcoding for identification of anuran species in the central region of South America

Koroiva, None Ricardo; Rodrigues, Luís Reginaldo Ribeiro; Santana, Diego José

doi:10.7717/peerj.10189

Cited by 9 publications

(7 citation statements)

References 59 publications

(66 reference statements)

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“…The molecular species delimitation results were identical to the morphological results for most of the species we examined. The presence of divergent results is commonly used to indicate possible cryptic species (e.g., [63]); in turn our identical results in terms of morphological, ABGD, and the occasional divergences suggest that COI has the ability to delimit in the evaluated species.…”

Section: Discussionsupporting

confidence: 68%

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

2022

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Odonates (Insecta: Odonata) are important insects in the food chains of freshwater environments around the world, being used as a model species for areas of behavior and analysis of environmental quality. In Brazil, especially in the Northeastern region, both knowledge about the distribution and molecular information of odonate species found in the two main biomes of the region is still limited. Aiming to improve these issues, here, we carried out an Odonata survey in two locations and built a DNA barcode database for species from the state of Paraíba. In total, 15 first records were reported for this Brazilian state and 142 specimens from 27 genera and 45 species had their ‘Folmer’ cytochrome c oxidase subunit I (COI) fragment evaluated. The database we generated includes data for 70% of the Odonata species found in Paraíba state. For 16 species, this is the first DNA barcode available in public sequence repositories. Our results demonstrate that using the COI in the regional scale can help identify and delimit those evaluated. Eight species (17%) showed a low percentage of differentiation (<2%) compared to other species currently deposited in the GenBank or BOLD System; nevertheless, we present morphological traits that reaffirm our identifications. Barcode data provide new insights into Neotropical diversity and deliver basic information for taxonomic analyses.

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Section: Discussionsupporting

confidence: 68%

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

2022

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“…Total DNA from the samples was extracted using the Blood & Tissue DNA Mini Kit (Ludwig Biotec, Alvorada, Brazil) from a small piece of muscle or liver tissue preserved in ethanol according to standard DNA barcoding methods for anurans (Koroiva et al, 2020). A total of 658 bp were amplified from the 5′ region of the mitochondrial cytochrome c oxidase subunit 1 (COI) gene using the T3-AnF1 (5-ATT AAC CCT CAC TAA AGA CHA AYC AYA AAG AYA TYG G-3′) and T7-AnR1 ('5-AAT ACG ACT CAC TAT AGC CRA ARA ATC ARA ADA RRT GTT G-3′) primers (Lyra et al, 2017 Lyra et al, 2017).…”

Section: Laboratory Proceduresmentioning

confidence: 99%

Late Pliocene population divergence and persistence despite Pleistocene climatic fluctuations in the Rio Doce snouted Treefrog (Ololygon carnevallii)

Myers

Folly

Ragalzi

et al. 2021

J. Zool. Syst. Evol. Res.

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The causes of population genetic divergence within the Atlantic Forest of South America are diverse. For example, studies have pointed to the importance of regions of stable suitable habitat throughout the Pleistocene, large rivers acting as biogeographic barriers, as well as changes in elevation. Here, we generate a phylogeographic dataset for the Rio Doce Snouted Treefrog (Ololygon carnevallii), a species that is endemic to a narrow portion of the Atlantic Forest. Gene-tree analyses demonstrate that this species is composed of three distinct lineages that diverged from one another during the Pliocene. Ecological niche models projected to climate during the Pleistocene and mid-Holocene suggest that regions of suitable habitat would have shifted through time since the last glacial maximum, and regions of stable habitat were identified. Using generalized dissimilarity modeling, we find no association between genetic divergence and ecological niche models, riverine barriers, elevation, slope, or current climate suggesting that none of these variables have been responsible for lineage formation in the Rio Doce Snouted Treefrog. We suggest that additional phylogeographic studies of narrowly endemic species within the Atlantic Forest are needed to better understand the drivers of diversification and accumulation of biodiversity.

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“…To compute phylogenetic diversity, we first built a community phylogenetic tree, using sequences from (Koroiva et al 2020), which are samples of individuals from our study localities. Additionally, five sequences from molecular samples were used from literature data (Table S1).…”

Section: Interaction Functional and Phylogenetic Diversitymentioning

confidence: 99%

Decoupled responses of biodiversity facets driven from anuran vulnerability to climate and land use changes

Ceron¹,

Sales²,

Santana³

et al. 2022

Preprint

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Anthropogenic climate and land use changes are the main drivers of biodiversity loss, promoting a major reorganization of the biota in all ecosystems. Biodiversity loss implies not only in the loss of species, but also entails losses in other dimensions of biodiversity, such as functional diversity, phylogenetic diversity and the diversity of ecological interactions.Yet, each of those facets of biodiversity may respond differently to extinctions. Here, we examine how extinction, driven by climate and land-use changes may affect different facets of diversity (functional, phylogenetic, and interaction diversity) by combining empirical data on interaction networks between anurans and their prey, species distribution modeling and extinction simulations.We used species distribution modeling to forecast the redistribution of anurans and create a species vulnerability rank based on expected range changes, then we simulate the extinction of anurans based on this rank. Next, we computed the variation in the functional, phylogenetic, and interaction diversities resulting from projected extinctions in four different ecoregions in the Neotropics. We found that the anuran vulnerability to climate and land-use change varies according to the level of trophic specialization. We also found a mismatch in the response of functional, phylogenetic, and interaction diversity to species’ extinction, whereby the effects on interaction diversity are stronger than those on phylogenetic and functional diversity. Although it is often assumed that interaction patterns are reflected by functional diversity, assessing the interaction patterns is necessary to understand how species loss may translate into the loss of ecosystem functions.

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DNA barcoding for identification of anuran species in the central region of South America

Cited by 9 publications

References 59 publications

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

DNA Barcoding and New Records of Odonates (Insecta: Odonata) from Paraíba State, Brazil

Late Pliocene population divergence and persistence despite Pleistocene climatic fluctuations in the Rio Doce snouted Treefrog (Ololygon carnevallii)

Decoupled responses of biodiversity facets driven from anuran vulnerability to climate and land use changes

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