DNA barcoding and genetic fidelity assessment of micropropagated Aenhenrya rotundifolia (Blatt.) C.S. Kumar and F.N. Rasm.: a critically endangered jewel orchid

Sherif, N. Ahamed; Kumar, T. Senthil; Rao, M. S.

doi:10.1007/s12298-020-00917-9

Cited by 17 publications

(3 citation statements)

References 50 publications

(48 reference statements)

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“…They promote rooting by changing the plant biochemical system (Henrique et al, 2006). According to Han et al (2009), auxin causes shoot buds to sprout, which in turn promotes the growth substances present in roots allowing them to expand and elongate (Sherif et al, 2020). Through repeated cell divisions, auxin administration causes the complex processes of lateral root development (Liu et al, 2002).…”

Section: Discussionmentioning

confidence: 99%

Efficient regeneration of in vitro derived plants and genetic fidelity assessment of Phalaenopsis orchid

Sarmah,

Mohapatra,

Seleiman

et al. 2024

Front. Sustain. Food Syst.

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This study uses inflorescence stalk node as explants to establish an efficient and quick Phalaenopsis orchids cloning procedure for the most significant monopodial orchid in floriculture, without callus formation. The current study aimed to develop a rapid and easy regeneration process utilizing flower stalk nodes as explants, while also evaluating the clonal fidelity of the in vitro micropropagated plants through the analysis of RAPD markers. The tissue-cultured plantlets were grown on a solidified half-strength Murashig and Skoog (MS) base medium enriched with 15% coconut water (CW), 150 mg L−1 activated charcoal, and a mixture of 6-benzylaminopurine, BAP (cytokinins) and α-napthalene acetic acid, NAA and indole 3-butyric acid, IBA (auxins). After 14 weeks of growth, the early production of shoot bud was reported in ½ MS medium enriched with 2.5 mg L−1 BAP alone. Maximum shoot bud multiplication was observed in ½ MS fortified with BAP (2.5 mg L−1) + NAA (1.0 mg L−1), while the lowest was observed in 1.5 mg L−1 BAP + 0.5 mg L−1 IBA after 4 months of culturing. In this investigation, roots emerged simultaneously with shoot elongation from the axil, indicating the absence of a distinct rooting stage. The largest number of roots (3.25) was produced by BAP (2.5 mg L−1) + IBA (1.0 mg L−1) compared to NAA. Control, on the other hand, displayed no signs of root growth. Tissue cultured plantlets with well developed root systems while planted in a potting mixture of brick and charcoal (1: 1) resulted in a 70% survival rate during hardening. The clonal faithfulness of in vitro regenerated crop plantlets to the mother plant was demonstrated by the DNA extraction method with ten micropropagated plants’ young leaves as well as the mother plant using random amplification of a polymorphic DNA marker.

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Section: Discussionmentioning

confidence: 99%

Efficient regeneration of in vitro derived plants and genetic fidelity assessment of Phalaenopsis orchid

Sarmah,

Mohapatra,

Seleiman

et al. 2024

Front. Sustain. Food Syst.

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“…The type of explant, concentration and types of growth regulators, number and duration of subcultures might influence genetic and epigenetic functions in plant tissue and might cause somaclonal-variation-based changes in propagated plants [21]. Molecular markers such as random amplified polymorphic DNA (RAPD), inter simple sequence repeat (ISSR), start codon targeted (SCoT) and amplified fragment length polymorphism (AFLP) have been used to assess the genetic stability in micropropagated plants of several orchid species [22][23][24][25]. Among these, inter simple sequence repeat (ISSR) markers have successfully revealed a high monomorphism percentage of the in vitro propagated plants to be similar to the mother plants in jewel orchids including A. formosanus [26,27] and A. elatus [28,29].…”

Section: Introductionmentioning

confidence: 99%

Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood & A.L. Lamb

et al. 2022

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Habitat disturbance and excessive collection of wild orchids from their natural habitat have threatened many orchids species at risk of extinction. In this study, the in vitro regeneration protocol for Macodes limii, a jewel orchid endemic to Sabah was established. The effects of explant source and plant growth regulators (PGRs) including naphthaleneacetic acid, picloram, 2,4-dichlorophenoxyacetic acid, 6-benzylaminopurine, kinetin, and thidiazuron on the in vitro regeneration capacity of M. limii plantlets were examined. Both factors showed a significant interaction in promoting axillary shoot formation. Nodal explants from the third and fourth positions cultured with 1.0 mg/L TDZ, induced 95% of shoot regeneration, with an average of three shoots/explant (1.6–1.8 cm of shoot length) after 90 days of culture. The well-developed plantlets went through an acclimatization phase for 60 days with a 60% of survival rate. An inter simple sequence repeat (ISSR) marker analysis confirmed the genetic stability of the in vitro regenerated plants to the mother plant. The successfully acclimatized plantlets were finally transferred to Poring Orchid Conservation Centre for reintroduction. The established protocol provides the means for large-scale production of this endemic jewel orchid, as well as a basis for further research aimed at the conservation and genetic improvement of this plant.

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“…al. 2022b; Gilman and Watson 2014; Unnikrishnan 2023).Research in other crops has indicated that a genetic similarity exceeding 90% between the micropropagated plantlets and donor plant is generally regarded as acceptable, assuming no phenotypic differences between the propagules and the parent plants(Bhattacharyya et al 2017;Sherif et al 2020;Tikendra et al 2019). Therefore, this protocol offers a reliable platform for mass propagation by growers and for conducting genetic transformation in L. speciosa.…”

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confidence: 99%

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

Wu,

Zhang,

Dixon

et al. 2023

Preprint

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Crapemyrtle (Lagerstroemia sp.) is the top-selling flowering tree in the U.S. However, threats from arthropod pests, including the recently emerged crapemyrtle bark scale (CMBS; Acanthococcus lagerstroemiae), severely jeopardize the aesthetic and production attributes of crapemyrtle. A tropical species, L. speciosa (L.) Pers. (“Queen's Crapemyrtle”) exhibits partial resistance to CMBS and other pests, but conventional breeding to incorporate the characteristics of L. speciosa into existing hybrids remains challenging. Recognizing the potential of tissue culture in facilitating molecular breeding, but also the possibility of undesirable somaclonal variations from in-vitro organogenesis, we utilized leaf explants of L. speciosa to develop a callus-induced regeneration protocol and assessed genetic fidelity of regenerated plantlets using inter-simple sequence repeat (ISSR) markers. Using woody plant medium (WPM) supplemented with 0.2 mg/L 2,4-D and 1.0 mg/L 6-BA achieved 97.9% callus induction. Shifting the growth regulators to 10.0 mg/L 6-BA and 0.5 mg/L NAA resulted in 32.4% of callus explants differentiating into adventitious buds. Finally, nodal segment proliferation (94.6%) and new shoot growth was maximized by using WPM supplemented with 1.0 mg/L 6-BA and 0.02 mg/L NAA. Explants rooted 100% using half-strength WPM supplemented with 0.2 mg/L IBA, and acclimatization survival was 98.3%. The ISSR primer analysis revealed 98.7% monomorphic markers, confirming the genetic integrity of the regenerated plantlets. We describe a reliable callus-induced regeneration system for L. speciosa, which will facilitate future molecular breeding and biotechnology to enhance cold hardiness, pest resistance, and other desired traits in this important genus.

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DNA barcoding and genetic fidelity assessment of micropropagated Aenhenrya rotundifolia (Blatt.) C.S. Kumar and F.N. Rasm.: a critically endangered jewel orchid

Cited by 17 publications

References 50 publications

Efficient regeneration of in vitro derived plants and genetic fidelity assessment of Phalaenopsis orchid

Efficient regeneration of in vitro derived plants and genetic fidelity assessment of Phalaenopsis orchid

Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood & A.L. Lamb

Reliable callus-induced plantlet regeneration from leaf explants of Lagerstroemia speciosa and genetic fidelity assessment through ISSR markers

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