Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood &amp; A.L. Lamb

David, Devina; Rusdi, Nor Azizun; Mokhtar, Ruzaidi Azli Mohd; Faik, Ainol Azifa Mohd; Gansau, Jualang Azlan

doi:10.3390/horticulturae8020155

Cited by 6 publications

(3 citation statements)

References 52 publications

(58 reference statements)

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“…protocorm-like bodies (PLBs)) has been routinely used for mass commercial production and regeneration of endangered orchids [ 197 ]. Propagation protocols have been established for many species with commercial values and conservation purposes [ 198 , 199 ]. In spite of the advantages, somaclonal variations induced by prolonged clonal propagation and cryptic genetic effects under microenvironment become the major limitations for maintaining certain desired traits [ 200 ].…”

Section: Biotechnology-aided Orchid Productionmentioning

confidence: 99%

Advances and prospects of orchid research and industrialization

Zhang

Zhao

et al. 2022

Horticulture Research

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Orchidaceae is one of the largest, most diverse families in angiosperms with significant ecological and economical values. Orchids have long fascinated scientists by their complex life histories, exquisite floral morphology and pollination syndromes that exhibit exclusive specializations than any other plants on earth. These intrinsic factors together with human influences also make it a keystone group in biodiversity conservation. The advent of sequencing technologies and transgenic techniques represents a quantum leap in orchid research, enabling molecular approaches to be employed to resolve the historically interesting puzzles in orchid basic and applied biology. To date, 15 different orchid genomes covering four subfamilies (Apostasioideae, Vanilloideae, Epidendroideae and Orchidoideae) have been released. These genome projects have given rise to massive data that greatly empowers the studies pertaining to key innovations and evolutionary mechanisms for the breadth of orchid species. The extensive exploration of transcriptomics, comparative genomics, and recent advances in gene engineering have linked important traits of orchids with a multiplicity of gene families and their regulating networks, providing great potential for genetic enhancement and improvement. In this review, we summarize the progress and achievement in fundamental research and industrialized application of orchids with a particular focus on molecular tools, and make future prospects of orchid molecular breeding and post-genomic research, providing a comprehensive assemblage of start-of-art knowledge in orchid research and industrialization.

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Section: Biotechnology-aided Orchid Productionmentioning

confidence: 99%

Advances and prospects of orchid research and industrialization

Zhang

Zhao

et al. 2022

Horticulture Research

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“…Even though a vast majority of orchids are frequently multiplied using tissue culture, Phalaenopsis orchids are one of the few economically significant genera that is reticent and causes difficulties for rapid clonal propagation (Singh et al, 2007;David et al, 2022). In addition to meristem culture, flower stalks, internodal segments of flower stalks, flower stalks with axillary bud, root tip (Park et al, 2002), and leaf segments (Park et al, 1996), numerous tissue culture procedures have been established for Phalaenopsis orchids.…”

Section: Introductionmentioning

confidence: 99%

Efficient regeneration of in vitro derived plants and genetic fidelity assessment of Phalaenopsis orchid

Sarmah,

Mohapatra,

Seleiman

et al. 2024

Front. Sustain. Food Syst.

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This study uses inflorescence stalk node as explants to establish an efficient and quick Phalaenopsis orchids cloning procedure for the most significant monopodial orchid in floriculture, without callus formation. The current study aimed to develop a rapid and easy regeneration process utilizing flower stalk nodes as explants, while also evaluating the clonal fidelity of the in vitro micropropagated plants through the analysis of RAPD markers. The tissue-cultured plantlets were grown on a solidified half-strength Murashig and Skoog (MS) base medium enriched with 15% coconut water (CW), 150 mg L−1 activated charcoal, and a mixture of 6-benzylaminopurine, BAP (cytokinins) and α-napthalene acetic acid, NAA and indole 3-butyric acid, IBA (auxins). After 14 weeks of growth, the early production of shoot bud was reported in ½ MS medium enriched with 2.5 mg L−1 BAP alone. Maximum shoot bud multiplication was observed in ½ MS fortified with BAP (2.5 mg L−1) + NAA (1.0 mg L−1), while the lowest was observed in 1.5 mg L−1 BAP + 0.5 mg L−1 IBA after 4 months of culturing. In this investigation, roots emerged simultaneously with shoot elongation from the axil, indicating the absence of a distinct rooting stage. The largest number of roots (3.25) was produced by BAP (2.5 mg L−1) + IBA (1.0 mg L−1) compared to NAA. Control, on the other hand, displayed no signs of root growth. Tissue cultured plantlets with well developed root systems while planted in a potting mixture of brick and charcoal (1: 1) resulted in a 70% survival rate during hardening. The clonal faithfulness of in vitro regenerated crop plantlets to the mother plant was demonstrated by the DNA extraction method with ten micropropagated plants’ young leaves as well as the mother plant using random amplification of a polymorphic DNA marker.

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“…More recently, a study from Malaysia reported the application of this marker in confirming the genetic stability of the in vitro regenerated plants to the mother plant, Macodes limii J.J. Wood & A.L. Lamb., a jewel orchid endemic to Sabah, Malaysia (David et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Investigation of the genetic diversity of jewel orchid in Vietnam using RAPD and ISSR markers

Tran

PHAM²,

TRINH³

et al. 2022

Biodiversitas

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Abstract. Tran TKP, Pham MH, Trinh TH, Widiarsih S, Ho VT. 2022. Investigation of the genetic diversity of jewel orchid in Vietnam using RAPD and ISSR markers. Biodiversitas 23: 4816-4825. Jewel orchid is a general name for several genera belonging to the Orchidaceae family, namely Anoectochilus, Doddinia, Goodyera, Ludisia, and Macodes. These plants are generally used as medicinal herbal for common diseases in Vietnam. Due to over-exploitation, the natural resources of jewel orchids are gradually depleted. Therefore, genetic resource research for proper development of this medicinal plant is necessary. This study aimed to investigate the genetic diversity of jewel orchids in Vietnam using RAPD and ISSR markers to provide basic data conservation of genetic resources and the development of this medicinal plant. A total of 20 jewel orchid samples were genetically characterized by using 10 RAPD and 10 ISSR primers. The amplified bands were binary coded and used to construct phylogenetic trees with NTSYSpc 2.1 software. Each RAPD primer produced 5-11 bands with an average of 8.0, and similarity coefficients ranged from 0.52 to 0.81. Each ISSR primer produced 5-10 bands with an average of 7.6, and the similarity coefficients ranged from 0.54 to 0.87. Combined RAPD and ISSR markers produced similarity coefficients ranging from 0.53 to 0.83. Based on the PIC values, the primers used by both markers are highly informative. However, the topology of cluster analysis of 20 jewel orchid accessions does not correspond to the taxa studied. The findings could be potential to employ in future classification, conservation, and development of this plant.

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Establishment of In Vitro Regeneration Protocol for Sabah’s Jewel Orchid, Macodes limii J.J. Wood & A.L. Lamb

Cited by 6 publications

References 52 publications

Advances and prospects of orchid research and industrialization

Advances and prospects of orchid research and industrialization

Efficient regeneration of in vitro derived plants and genetic fidelity assessment of Phalaenopsis orchid

Investigation of the genetic diversity of jewel orchid in Vietnam using RAPD and ISSR markers

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